FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_338_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_338_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3547032
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA121420.3423143631069582No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA53840.1517888758827098No Hit
TTCTCAGTATGGAAACAGGTGTTGCCATATTGTAAAGAGCCATGGCTTCG41480.11694284122612934No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG38370.108174947392637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA25500.092.621684
AAACACG27150.086.456215
AACACGA21800.079.26566
AGCACGC5700.077.0891341
AACACGT9600.070.49966
CGCACTT5400.069.335875
ACACGAG25450.068.46237
ACGAGCC12800.065.255159
TAGTAAC38750.064.3049852
CACGAGC28300.061.058878
CATAACC32200.060.1487276
GAAACAC39700.059.6738974
GAAGCGG11850.058.9646381
TAGAAAC57150.058.8492432
ACGCACT8450.058.79924
GCACGCA7500.058.5745352
CGGTGCG1154.1836756E-1156.361812
ACACGTG13300.050.8862157
AACCTCC38100.050.8386579
GTAGAAA72600.050.0071531
Produced by FastQC (version 0.11.2)