FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_337_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_337_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733152
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTCTCTGCTTGTGATTCTTTGGCTTTGTATCTAGGAGTGGAAGCGC192602.6270132250883855No Hit
TTGAGTTAGGGAGTTACTGATCTCTCTGGTAACTGAACAGGTTTAGAAGT126431.724471869407708No Hit
TTGAGGTCTGGAAATTTAGTTTCTAACAAGTTTGTTTGTTGAAAAGGGCA117291.5998046789751648No Hit
TTGAGTTGAAGAAATTGGTGGCAAATTCTGCCTCCTATAATATTACACTT99401.3557897952948366No Hit
TTAAGATCCTATCTGAAGTTGAAGTGTATGGGTCACACGGCCGGCTTCAA96361.3143249967264632No Hit
TCAAGTACAGAATATAGATACAGCTTAAATTTTGTCTTACAAAGTCAGCA95341.3004124656278644No Hit
TCAAGGCCCATGGTCCGTGCTCGCTTCCAGCTCCAGCTTCCCGCTTGGGA91361.2461263148705861No Hit
TTGAGTTTCTGGGATTACAGCCATAAGCCTGGCCTGGTAAATTTCAAGCA62330.8501647680153638No Hit
TCAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCT59750.8149742481777312No Hit
TTGAGTGAAGAAGGCACAGGTCTTAAAATAGGTTTTCTTGCAGGGAGAAC49700.6778948976474183No Hit
TTGAGTTCGGAAGAAATGAATATTCCAAATTTCCAATTTTTACAATATCT48670.6638459691851075No Hit
TTGAGTTCAAATGTGGCAGTGTTCCAGAAGTAGTACTGTGCTGCATATCA48140.6566169089083846No Hit
TTAAGAAGACAGAAGGGCAAAGCCACAGGTGGTAAAGTCATGATTGTTGA47040.6416131989000917No Hit
TTGAGCCTTTGTGTCCAAAGCAAGAACTACTTCTGGATTTTTAGTCAAAT46530.6346569333507922No Hit
TCAAGTGTGGCCCTAGCACCAAGGAAGCTTCATTAGTGATTTACCTACTA45520.620880799615905No Hit
TTGAGTCCTGTTTGGAGGACAGCTGCTCCCACATCTGGGGGCCTCTGGAT44310.6043767186067828No Hit
TCGAGCATACAAAATAGGGAAAAGCACAAATCTGATAAGAGAAATACCCT43210.5893730085984897No Hit
TTGAGTCCAGTCCTGGTTCTATCTCTTAATACCTGTACCGTTTTGTACAA42770.5833715245951726No Hit
TTGAGCCAGAAAAGCAAAGCCCTCTAGAACCCAGCTGCTCCAAGTGTGGT33410.4557035921609707No Hit
TTGAGTTCGGAAGATTCCAAATTTCCAATTTTTACAATATCTAGTTAAAT27650.3771387106630003No Hit
TTGAGTGGCTGGCTGGAGACTGGAACATATACACTGGCATACAATTATAT27230.37141002138710666No Hit
TCGAGCTGTTGAGGCGTGTCAGGAAATCTAGCAACATCTGTATCCTCCCC24230.3304908122735804No Hit
TTGAGTTCGGAAGGAGAGGGCCCGTGATGAAACACTTTCTCCTGCTTTCA23870.32558050717995723No Hit
TTGAGTTCGGAAGGACAAAGAAATGAATATTCCAAATTTCCAATTTTTAC23110.3152143075378639No Hit
TTGAGTTTGGAAGAGTTGGAGAACTTCTGAATTTAAGCAGGCAAAAATGA22530.30730326044258216No Hit
TTGAGTTTTGATGGGAAATCATTGGGCATCCACCTTTCAGTCCTGATTTG22090.301301776439265No Hit
TTAAGCCAAAGCCCACGGCAATTCTGGCTGCTTTCTCCTGAGGACCACAT21590.29448190825367726No Hit
TTGAGTCCGGAGGCAGAGGTTGCAGGTTGCAGTCAGTCAAGATCGCACCA19470.26556566714678537No Hit
TTGAGACCAACTCACAGAGGCTGGGACTCTGATTCAAGCTTAGGTTGCTG19040.2597005805071799No Hit
TCAAGGCATTTTGCTTTTTGACTTTCTGGAGGGTGAAAGAATGATAACAT18890.25765462005150364No Hit
TTGAGTCTTTGTGTCCAAAGCAAGAACTACTTCTGGATTTTTAGTCAAAT18700.2550630701409803No Hit
TTGAGTACGGTGTGATCAAGGACCAAATCAATTACATAGTCAGTTGAGAC17770.24237811531578718No Hit
TCAAGCCAGTCTCTAATATCGTTAATGCATTTGATAAGATAAAATCATTA17760.2422417179520754No Hit
TTAAGATTATCTCAGGCCCTGAACTTTTCAATTCACAAGTGAAATTCTAT17660.24087774431495787No Hit
TCAAGGGGGTACAAGGGGAAGCATCTTTAAGATGAAGGAAAGTCAGGAAC17350.23664942603989347No Hit
TTGAGTTGCTGTCTCTTTCCCTCCCTTCCCCTGGCAGACAGAACCCTTCA17020.23214831303740563No Hit
TTGAGTTCGGAAGAAGGAGAGGGCCCGTGATGAAACACTTTCTCCTGCTT16730.22819278948976474No Hit
TTGAGTGCTAACCCAAGGTTAAAAGCTCATGTGATGCTGTCCAAATCATT16370.2232824843961416No Hit
TTGAGCATCCCAAAATGTTGCCATGATCTTTGCTCTTGACTGGTCCGCAT16320.22260049757758282No Hit
TTGAGTATCCCAAAATGTTGCCATGATCTTTGCTCTTGACTGGTCCGCAT16000.21823578193880666No Hit
TTGAGTTGGAGAACTTCTGAATTTAAGCAGGCAAAAATGAACTTATTTAT15140.20650560865959583No Hit
TTAAGTGGTTTGAAGAACAGTAGGGAAGAGAACTATGGCTTGCCTTCCCT14900.20323207193051374No Hit
TCAAGGCTCTTTGTTGCGGCCGGGCGCCGTGGCTCACGCCTATAATCCCA14730.2009133167474139No Hit
TTAAGTTCCATCAAGTTCAAGACACTTTTATAAGTGGTACTACCAGCCAC14540.19832176683689057No Hit
TTGAGTAAGAAAGCATTCCGGTTCCAACTTGGTCAACGAGTCACTTTTAG14480.19750338265462006No Hit
TTGAGTGGTTCTCTATGTGTACATTGTCTTTGGAATGATTGAGAGGTGGA14070.19191109074243812No Hit
TCAAGTGAGGAAAATGGAACTTGAAGAACTTTATATTCTTTGTCTGTATG13800.18822836192222075No Hit
TTGAGTTAAGAAGGTGAGAGCTCAGTCTTCCCTCCTCACACCTCAGTTTT13180.17977172537209202No Hit
TCAAGTTTGCCAGGATATGATAAAAGGATATATTTATATGTGTGTGTATA12700.1732246519139278No Hit
TTGAGTTTATCTGAATTTTTCTCGTGTCTTCCTGCTCCTCCTTCCTTGTA12480.1702239099122692No Hit
TTGAGTTTGTAAGCCCCAAATATTGTAATAATTGTGCTTGAAACCATAAG12080.16476801536379904No Hit
TTGAGTCTTTGAGAACAGTTTTTTACTCCTTTATACCATTTTTTGTTTAG11500.1568569682685173No Hit
TTGAGTCCAATAGCTGCCCCAGCTTACCACATTAATTAGTCTTCAGCCGA11390.155356597267688No Hit
TTGAGCGGCTCCAACATCTCCCACCAGTGCCGGCATTCCTGGGCAGGTGA11240.1533106368120117No Hit
TTGAGTTCCATCAAGTTCAAGACACTTTTATAAGTGGTACTACCAGCCAC11230.15317423944829994No Hit
GCCGCCTTACACTGACTTGAGTTAGGGAGTTACTGATCTCTCTGGTAACT11220.15303784208458818No Hit
TTGAGTTCGGAAGGGAAATGCAAATATAAAACACAGTGAGATACCACCTC11180.15249225262974117No Hit
TTGAGTTTGGAAGCAGCTTAACTTTGGGTTGTATCAGGCAGTCAGGTTCC10830.14771834489982977No Hit
TTGAGTTCGGAAGGAGAGATTATCTTCTATGTATGTGCTAGCAGTAGCTC10650.1452631923530182No Hit
TTGAGTGGCTCCAACATCTCCCACCAGTGCCGGCATTCCTGGGCAGGTGA10650.1452631923530182No Hit
TTGAGTTCGGAAGACTGGAACATATACACTGGCATACAATTATATCAGTC10620.14485400026188294No Hit
TTGAGTGCGACTGAATAGTAGATCCTGGGGAAAAGTATAGTAGAACCAGA9920.13530618480206014No Hit
TTGAGTGGGGGTTGCACAGACCTGCTCATCTGCTCAGAAGAGGAGCTCAG9900.13503339007463663No Hit
TTGAGTTCGGAAGCCCCAAATATTGTAATAATTGTGCTTGAAACCATAAG9350.12753153507049014No Hit
TCAAGGGTGTAGACTTTATTTGGTAAGCAAAGGGCTGCACAGATGGATTT9140.1246671904325433No Hit
TTGAGTTGGGAAAGGGGATGGGAGTATCATCATTCCATGAGTAGATTTTT9110.12425799834140806No Hit
TTGAGAACCACACAAAAGATGCTGTGTCTTTCATATGTTCCTGGGTTGCT8840.1205752695211907No Hit
TTAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAATTCCAGATCCATCCT8770.11962048797520841No Hit
TTGAGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCTTGC8540.11648334860983806No Hit
TTGAGTTCGGAAGAGATTAATATCCACAATATATAAAGAACTTCTACAAG8390.11443738815416177No Hit
TTAAGTTAAAAAAATAAAGTTCACATTTTTTATGTTCATTATGTAATTCA8160.11130024878879141No Hit
TTGAGGCTTCATTTCCCAAGTCAGTGATGCGTTCTGCAGCTAAGGTGGGC7930.10816310942342107No Hit
TTGAGTTGGGACGTTTGGGGGCTTTATATCTTTAAATATTCTTCCCAGTT7520.10257081751123913No Hit
TTGAGTTCGGAAGAGAAGCCCTCAGTGAGTTTTACAATACTCCCATTTAA7490.10216162542010389No Hit
TCAAGAGGCAGGGCAGTTGTATTCCGAACCCCAGTGGATACTTACTGAGC7490.10216162542010389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGC253.86608E-8144.005338
GCCGCCT29350.0136.974991
CGCCTTA29450.0135.711143
CCGCCTT29650.0135.561312
TTGAGTT375600.0134.138411
GAGTTCG252200.0133.231663
GTTCGGA246600.0132.939225
AGTTCGG247950.0132.921424
TTCGGAA244300.0132.54036
TGAGTTC282150.0131.248352
GTTCGAA3700.0130.38325
GCCTTAC30750.0129.730714
CGGAAGG64250.0129.436698
CTTACAC30900.0128.626116
TCGAGCA4450.0127.9034651
CGGAAGT35750.0127.691428
CCTTACA31250.0127.18555
GAGTCCG4000.0126.021843
GTTTCCG1600.0126.0046545
GTGCGAC1600.0126.0046545
Produced by FastQC (version 0.11.2)