FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_337_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_337_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3159994
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC56450.1786395796954045No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC54090.171171211084578No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT52760.16696234233356141No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA52450.16598132781264774No Hit
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG49170.15560156126878721No Hit
GTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAG38120.12063314044267173No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG37200.11772174250963768No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC36070.1141457863527589No Hit
CTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAAGCT35020.11082299523353525No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA109850.0120.1998141
TAGTAAC110650.0119.315662
GTAACAA113250.0117.254
AGTAACA125050.0106.1334843
TAACAAG128050.0103.632195
AAGAGCG21850.095.5687949
CGCACTT20300.092.220245
ACAAGAG148200.089.00467
AACAAGA157500.084.071756
CAAGAGC162150.080.770168
CTCGCTC24200.077.964463
ACGCACT24700.077.548764
GCACGCA24550.075.399422
TCGCTCT25100.075.165354
AGCACGC25050.072.753551
GTCTCGC27450.072.1658551
CGCTCTG28000.066.602515
AAACACG50750.065.4054265
TCTGTCG28650.065.340718
AAGCGGT4400.063.8407972