FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_328_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_328_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565776
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA367806.500805972681769No Hit
TTGAGTAGAAGTAACGATCTCTTTGATCAATGGGGGTATGAGGAATCACC216133.82006306382738No Hit
TTGAGGCTTGTATTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAA178583.1563728401346114No Hit
TTAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC153942.7208647945476656No Hit
TTGAGAAAATACCATGCACAAGATTATTAAATCTTTCATGTCCTCCTTCT102441.8106105596561184No Hit
TTGAGTGAAGTTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCA98221.7360227369135488No Hit
TTGAGTCCAGTCCTGGTTCTATCTCTTAATACCTGTACCGTTTTGTACAA78141.3811119595034078No Hit
TTAAGAGCACACAAGAAGCTATAAACAAGATAACAAAAAATCTCAACTCT74811.322254743926925No Hit
TCAAGAACTGATAAGGCTCCCCATTGCTCCTTAGCTTTGGTGGTGAGAAA59361.049178473459461No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTGTAGAATTCGCCCAGTTTCAC53720.9494923786092022No Hit
TTGAGTACTTTTCTAGACATTCTTTGGCTAATGTCTTCGGTTTTTTGGAC46950.8298337151098668No Hit
TTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGGA45930.8118053788071604No Hit
TCAAGACACTAGGATGTTCCAAGGAGTGAGGGGTGAAATAAATTTTCTTA45870.810744888436413No Hit
TTAAGATTCGAGAGGGCAGAATAATAAAAGAAATATTTCCAACAGGAAGA36530.6456618873900626No Hit
TTGAGTTTTGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCAT35230.622684596023868No Hit
TTGAGTGAAGTTCGATTGCCTTCATGAAAAATACGGTGGATTAAACAAAA34930.6173821441701309No Hit
TCGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG33180.5864511750233308No Hit
TTGAGTGAAGTTCTTTGGTTGTCCCCATCTCCCACATCTTTGGTGTATCA33020.5836232007013377No Hit
TTGAGTGAACTTCAAGTACCAGTAAAAGAACTGAAAATCAAAATGTCCAA30800.5443850569836827No Hit
TTGAGTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGGGTCATAC30210.5339569016713328No Hit
TTAAGTGGTTTGAAGAACAGTAGGGAAGAGAACTATGGCTTGCCTTCCCT28970.5120401006758858No Hit
TTGAGTGAAGTTCATTGCCTTCATGAAAAATACGGTGGATTAAACAAAAG25190.44522920731879756No Hit
TTGAGTGTAGTCAGATGCATTCATTAGTTCATTTATCCCATGTAGCTCAC23490.41518198014762026No Hit
TTGAGTCTCCATAGAGGTTCTTCATTTGGGTTTTGTTATCTGAATGGAAC23440.4142982381719974No Hit
TCAAGATGATGAATGACTCAATGGCTAAGAAAACCAGTGGAAATGCTTTC23340.4125307542207517No Hit
TTGAGCTAAGGACCTTTGGACCATCCATGCTATGGTTCTTGGCATCCCCT22850.40387008285964765No Hit
TCAAGAGCATGGAATAGAGACTCCTAAGTATCTGGCTGATTTGTTTGATT22470.3971536438449139No Hit
TCAAGTGCCAATAGATGCTCATCTTCACTGTTTATTATTCCTTCGTTGGA18640.3294590085122027No Hit
TTAAGGAATCTGCATTCACTAACCCCTGAAGCTGGGCCATCAGTTATCAT17840.3153191369022369No Hit
TTGAGTGAAGTTCTTGCCTTCATGAAAAATACGGTGGATTAAACAAAAGC16500.2916348519555442No Hit
TTGAGTAAAGTTCACCTCATCTGCTAATGGAGTAACCACACATTATGTTT15920.2813834450383191No Hit
TTGAGTGCCAAGTCGTTTTCCCACCATCATGTACCATTTCTATCCCAATA15140.26759707021860246No Hit
TTGAGTGAAGTTCAGATTGCCTTCATGAAAAATACGGTGGATTAAACAAA14790.2614108763892424No Hit
TCGAGCTGGATGAGAAAGTGGATGATCTCAGAGCTGACACTATAAGTTCA14210.25115946947201717No Hit
TTGAGTGAAGTTCAAGCCTAGATCAGCATTAAAATGCAAGGGTTTCCATG14190.2508059726817681No Hit
TTGAGTGAAGTTCTGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATC13110.23171714600831425No Hit
TTGAGTGAAGTTCGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCA13060.23083340403269137No Hit
GCCGCCTTACACTGACTTGAACTAAAGAGAATATCAGGAAGAGGATTCAA12230.2161632872373519No Hit
TTGAGTTCCCTTAAAAATAAATTAGAGAACAAAATTAAGTCAGAAAAAGC12200.2156330420519782No Hit
TGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGAC11170.19742795735414723No Hit
TTGAGTGACTGCAAGACCAGCTCTTCCCACTCCACCTTCCCATTGGGATC10940.19336274426628208No Hit
TTGAGTGAAGTTCAAAAGCCATAGGAAATTGCCCAATATGGGTGAAAACA10690.18894403438816776No Hit
TTGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG10530.1861160600661746No Hit
TTGAGTACTGTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAA9650.17056220129521224No Hit
TTGAGTGTGCATATCTGGTGTGTGTGTGTCTGTATGTCTGTGTATGAGTG9130.1613712847487345No Hit
TTGAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAA9080.1604875427731116No Hit
TTGAGTGAAGTTCTGCATCAACAGGCACAGAATTCAAGCCTAGATCAGCA9010.15925030400723963No Hit
TTGAGTAAAGTTCCACCTCCTTTAATTGCAACACCAGTCGCACCTGATCT8910.1574828200559939No Hit
TTGAGTAAAGTTGCAGAAATTATTCAGAAAGTAGCTGAAAAGGAGAGCTT8840.1562455812901219No Hit
TCAAGAACTGATAAGGCTCCCCATTGCTCCTTAGCTTTGGTTGTCCCCAT8290.14652441955827042No Hit
TTGAGTGAAGTTCTGCACCAGTTCTTTGGTTGTCCCCATCTCCCACATCT8230.14546392918752296No Hit
TTGAGTGAAGTTCTTTGTCTCTTAATTCCTTGTGAAATGTCATTGGATAA8090.14298945165577898No Hit
TTGAGTGAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGGGTCATAC8040.1421057096801561No Hit
TTGAGTGGACATACCCTCGTTTATCTTGCCCGGGATCAACCTTTCAGAAA7980.14104521930940866No Hit
TTGAGTAGAAGTAACGATCTCTTTGATCAATGGGGGTATGAGGAATCCCC7970.1408684709142841No Hit
TGAGTAGAAGTAACGATCTCTTTGATCAATGGGGGTATGAGGAATCACCC7800.13786374819716637No Hit
TTGAGTGAAGTTCACAGGAGAACATGCAAAAGCCATAGGAAATTGCCCAA7680.1357427674556715No Hit
TTGAGTGAAGTTCTAGTACCAGTAAAAGAACTGAAAATCAAAATGTCCAA7670.13556601906054694No Hit
TTGAGTGTGTCTGAGTGTATGTGTGAGTGTGCATATCTGGTGTGTGTGTG7030.1242541217725743No Hit
TTGAGTGAAGTTCTTCATGAAAAATACGGTGGATTAAACAAAAGCAAGCC6810.12036565707983372No Hit
TTGAGTGAAGTTCGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCAT6670.1178911795480897No Hit
TTGAGTGAAGTTCATGAAAAATACGGTGGATTAAACAAAAGCAAGCCTTA6590.11647719238709314No Hit
TTGAGTGAAGTTCGAGTACCAGTAAAAGAACTGAAAATCAAAATGTCCAA6520.11523995362122112No Hit
TTGAGTGAAGTTCTGCGTTAACAGGCACAGAATTCAAGCCTAGATCAGCA6470.11435621164559825No Hit
TTGAGTGAAGTTCCTTCATGAAAAATACGGTGGATTAAACAAAAGCAAGC6380.11276547608947711No Hit
TTGAGTGAAGTTCTGCCTTCATGAAAAATACGGTGGATTAAACAAAAGCA6290.11117474053335596No Hit
TTAAGGCATTTTGCCTGTTGACTTCCTGGAAGGTCAAAGAATGGTAATAT6080.10746302423573993No Hit
TTGAGTGAAGTTCGCCTGTGACATAATAAGAGCTGCATTAGGATTAAAGA5850.10339781114787477No Hit
TTGAGTGAAGTTCCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTT5770.1019838239868782No Hit
TTGAGTGAAGTTCGCCTTCATGAAAAATACGGTGGATTAAACAAAAGCAA5740.10145357880150449No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGC100.0072371303144.00766
GCCGCCT21800.0142.088641
CCGCCTT22000.0139.462512
CGCCTTA22100.0138.505573
CCTTACA22200.0137.520785
CTTACAC22100.0137.165626
TAAGGTC19950.0135.38142
GAGGCTT21100.0133.122883
AAGGTCT20300.0133.047233
GTGAAGT160500.0132.433155
TTAAGGT20300.0132.361041
ATACCAT12850.0132.240459
GAGTGAA182000.0132.213473
TGAGGCT21300.0131.872912
TTACACT23000.0131.485217
GTAAATT48700.0131.292365
TTGAGGC21700.0130.792821
TTGAGTA120700.0130.404191
AGTGAAG166650.0130.118074
TGAGTAA75250.0130.071932
Produced by FastQC (version 0.11.2)