FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_328_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_328_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2968522
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT38740.1305026541827886No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA38240.12881831429916976No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC37880.1276055895829642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC10000.080.687181
CGCACTT11700.072.005725
AACACGA21600.064.0018546
ACGCACT13500.063.4770554
AAACACG30950.062.118025
TAACAAG42600.061.8640675
CGGTGCA4500.060.8160932
GAAACAC32700.059.0188374
GCACGCA13950.057.821762
AACACGT9550.056.5461356
GTAACAA46900.055.7362024
ACGTGCC3800.054.951739
ACACGAG25550.054.67277
ACGAGCT4400.052.3677949
ACACGTG10500.052.1175467
CACGAGC28850.048.1685838
GCGGTGC11850.048.0280881
GTAGTAA60900.047.318311
TAGAAAC53450.045.67722
TAGTAAC63850.043.5385442
Produced by FastQC (version 0.11.2)