FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_310_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_310_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740014
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTGGCTCTGGGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCA9131112.339090882064394No Hit
TCAAGCTCACTCTCCGCCGCCCCTAAAGACCTCTTCCTCCCTCCTCTGAA172982.3375233441529484No Hit
TTGAGTTAACGCTGGACGAGATGAGGACTCAGAGTCAAGATCAAGTTGCT135101.8256411365190388No Hit
TTGAGTCAACCCTGAACAGGCCACTGATGTGTGATGTTCCCCTCCCTGTG97311.3149751220922847No Hit
TTGAGTTAACGCTCCGGGGGGAAAATCATGGGGAAAATACTTAAAATGTC79011.0676825033039916No Hit
TTGAGTTAACGCTGAGGTGAGGACAGGAGTTGGAAAAGGGCCTGCCTGCT78951.0668717078325545No Hit
TTGAGTTAACGCTGTGGCATGGCTGAGGTGAGGACAGGAGTTGGAAAAGG76391.032277767717908No Hit
TTGAGTGTTTTGGAGGGAAAAGCAGTTTGTAAATGTTTGTTGAATGACAG71940.9721437702529953No Hit
TTGAGTTTATCTGAATTTTTCTCGTGTCTTCCTGCTCCTCCTTCCTTGTA63630.859848597458967No Hit
TCAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCT57130.7720124213866224No Hit
TCAAGGATCTAGAACAAGAAATAACATTTGACCCAGTAATCCCATTACTG52910.7149864732288849No Hit
TTGAGTGAATCCTGCTGCTTGTTAGCCATCAGGGTTGGTGGTGGCACTGT49830.6733656390284508No Hit
TTGAGTTAACGCTGCAGAAATGGACAGCAGGCATCTCCAAAGTGACCTGT43270.5847186674846693No Hit
TTGAGTTGTGTCCAGGTTTTGCGTGGCCATGGAAAATCAGGTGAAGGACA42200.5702594815773756No Hit
TTGAGTTAACGCTGCAGGAATGGACAGCAGGCATCTCCAAAGTGACCTGT41940.5667460345344818No Hit
TTGAGTAAACAAATCTTAGTGAATTCAAGGACCTTCGAAAGAGGTTAGAG39810.5379627952984674No Hit
TTGAGTTAACGCTTTTCCTTATAAATTACACAATCTCAGTTATTTCTTTA33510.4528292707975795No Hit
TCAAGAACCCTGCAGAAATGGACAGCAGGCATCTCCAAAGTGACCTGTGT33470.45228874048328815No Hit
TTGAGTCCAATAGCTGCCCCAGCTTACCACATTAATTAGTCTTCAGCCGA33190.4485050282832487No Hit
TTGAGTTAACGCTGCAGACATGGACAGCAGGCATCTCCAAAGTGACCTGT32130.43418097495452784No Hit
TTGAGTCAAGGCAGATGAGCTGGAGAACACGGTGCTTCTGGGAAGCTGAA30810.41634347458291326No Hit
TTGAGTTAACGCTGCAGCAATGGACAGCAGGCATCTCCAAAGTGACCTGT29410.39742491358271603No Hit
TGAGTGGCTCTGGGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCAA28580.3862089095611705No Hit
TTGAGTTAACGCTGGGGGGAAAATCATGGGGAAAATACTTAAAATGTCTA27770.3752631706967706No Hit
TTGAGTGAACTTCCCCCCCAGTACGGGCACCCAGAGTTGGTGCCGCGCTC27570.37256051912531385No Hit
TTGAGTTAACGCTGGACGCGATGAGGACTCAGAGTCAAGATCAAGTTGCT27430.3706686630252941No Hit
TTGAGTCAAAGCTGCAATGTGGAGAGATGAAGGAAAAGAGAGAGCCAAAT26200.3540473558608351No Hit
TCAAGGGCCTTTACTTTTGCAATTTTCCCTGCTTGGAGCAATCTTCCTCC26070.3522906323393882No Hit
TTCAGGAGGGTAATGAGAGTTGCAGCAAGTATAAATCTTGTCACAACCCC25540.3451286056750278No Hit
TTGAGTTAACGCTGCAGATATGGACAGCAGGCATCTCCAAAGTGACCTGT25230.3409394957392698No Hit
TTGAGTTAACGCTGCAGAGATGGACAGCAGGCATCTCCAAAGTGACCTGT23380.3159399687032948No Hit
TTGAGTTAACGCTGCTGAAATGGACAGCAGGCATCTCCAAAGTGACCTGT22960.3102644004032356No Hit
TTGAGTTAACGCTGCCGAAATGGACAGCAGGCATCTCCAAAGTGACCTGT22270.3009402524817098No Hit
TTGAGTTAACCCTGCAGAAATGGACAGCAGGCATCTCCAAAGTGACCTGT21880.2956700819173691No Hit
TTGAGTTAACGCTAGCTGGACGAGATGAGGACTCAGAGTCAAGATCAAGT21160.28594053626012483No Hit
TTGAGTCCTGTTTGGAGGACAGCTGCTCCCACATCTGGGGGCCTCTGGAT20320.27458939966000645No Hit
TTGAGTTAACGCTTGCAGAAATGGACAGCAGGCATCTCCAAAGTGACCTG19980.26999489198852994No Hit
TTGAGTTGGAAAAGGGCCTGCCTGCTGAAAGGCTCCAGTCCTGTGTGTTG19350.26148153953844117No Hit
TTGAGTAAACACAATCTGCGGGTAATTAGCATCATTCCACCTTTCAGGGA18910.25553570608123627No Hit
TTGAGTTAACGCTGCCAGCTGGACGAGATGAGGACTCAGAGTCAAGATCA18560.250806065831187No Hit
TTGAGTTAACGCTGGAGAAATGGACAGCAGGCATCTCCAAAGTGACCTGT18160.2454007626882735No Hit
TTGAGTTTACGACTGTCGACCATTTCCCATGATCCAAACAGAGCCTAAGA17920.24215758080252536No Hit
TTGAGTTAACGCTGGAGACTGGAACATATACACTGGCATACAATTATATC17780.24026572470250562No Hit
TTGAGTTAACGCTGCCTGCTGAAAGGCTCCAGTCCTGTGTGTTGTGGTTG17720.23945492923106862No Hit
TTGAGTTAACGCTGTAGAAATGGACAGCAGGCATCTCCAAAGTGACCTGT17580.23756307313104888No Hit
TTGAGTTAAGGCTCCAGTCCTGTGTGTTGTGGTTGGGTCTGTCACTGAGC17330.23418475866672792No Hit
TTGAGTTAACGCTGCAGTAATGGACAGCAGGCATCTCCAAAGTGACCTGT17070.23067131162383417No Hit
TTGAGTGGCTGGCTGGAGACTGGAACATATACACTGGCATACAATTATAT16760.22648220168807617No Hit
TCAAGGCTCTTTGTTGCGGCCGGGCGCCGTGGCTCACGCCTATAATCCCA16060.2170229211879775No Hit
TTGAGTTAGTTTGCTGAGAATGGTGGTTTCCAGCTTCATCCATGTCACTG15480.20918523163075292No Hit
TTGAGTGGCTCTGGGAGGTCATGAGGGAGGGGACATGGGAATGCGCCCCA14410.19472604572345928No Hit
TTGAGTTAACGCTGAAGAAATGGACAGCAGGCATCTCCAAAGTGACCTGT13330.1801317272375928No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTGTAGAATTCGCCCAGTTTCAC13190.17823987113757309No Hit
TTGAGTTAACGCTGCTGGAATGGACAGCAGGCATCTCCAAAGTGACCTGT12260.16567254133029916No Hit
TTGAGTTAACGCTTTAATTTGCATCTTTTACTGATATTTTTGGTATAGTT12230.16526714359458064No Hit
TTGAGTTAACGCTGGCTGGAGACTGGAACATATACACTGGCATACAATTA11910.16094290108024983No Hit
TTGAGTTAACGCTGAAATGGACAGCAGGCATCTCCAAAGTGACCTGTGTA11230.15175388573729687No Hit
TCAAGTCTTCACAAGCGCTGATCATTTTTACCACCTGTAAGATACCTCTT10480.14161894234433403No Hit
TTGAGTTCAAGCTTCAGAGGAGGGAGGAAGAGGTCTTTAGGGGCGGCGGA10030.13553797630855632No Hit
TTGAGTTAACGCTGGACGGGATGAGGACTCAGAGTCAAGATCAAGTTGCT10000.1351325785728378No Hit
TTGAGTTAACGCTGCATAAATGGACAGCAGGCATCTCCAAAGTGACCTGT9920.1340515179442551No Hit
TTGAGTTAACGCTGCAAGAAATGGACAGCAGGCATCTCCAAAGTGACCTG9660.13053807090136132No Hit
TTGAGCCTTTGTGTCCAAAGCAAGAACTACTTCTGGATTTTTAGTCAAAT9610.12986240800849713No Hit
TTGAGTTAACGCTGCAAAAATGGACAGCAGGCATCTCCAAAGTGACCTGT9590.12959214285135146No Hit
TTGAGTTAACCCTGAACAGGCCACTGATGTGTGATGTTCCCCTCCCTGTG8710.11770047593694173No Hit
TTGAGTTAACGCTCACCCCAGATGCCCTGGCACTATGGACGGCACCAGGA8660.11702481304407754No Hit
TTGAGTTAACGCTGAGTTGTGTCCAGGTTTTGCGTGGCCATGGAAAATCA8560.11567348725834918No Hit
TTGAGTTAACGCTAGGACCTCAAAAAGAGACACCAAGAGCTTTCTCCTGG8490.11472755920833931No Hit
TTGAGTTAACGCTGCGAAATGGACAGCAGGCATCTCCAAAGTGACCTGTG8460.1143221614726208No Hit
TTGAGAATTTAGGGGGTGGTCACATGGTACTGTGCAACAGGAAGTGATAT8440.11405189631547512No Hit
TTGAGTTAACGCTGCGAGAAATGGACAGCAGGCATCTCCAAAGTGACCTG8310.11229517279402822No Hit
TTGAGTTAACGCTAATGCCCATCAATAATAGACTGGACAAAGAAAACGTG8200.110808714429727No Hit
TTAAGATCCTATCTGAAGTTGAAGTGTATGGGTCACACGGCCGGCTTCAA8180.11053844927258133No Hit
GCCGCCTTACACTGACTTGAGTGGCTCTGGGAGGTCATGAGGGAGGGGAC8080.10918712348685294No Hit
TTGAGTGGCTCCAACATCTCCCACCAGTGCCGGCATTCCTGGGCAGGTGA8050.10878172575113443No Hit
TTGAGGTGGACATGCCATTTTAGTGTGGTTGTTTTGGGGTTAAAAGGATA7980.10783579770112459No Hit
TTGAGTTTGCTGCACCCATCAACCTGTCGTCTACATTAGGTGTTTCTCCT7810.10553854386538633No Hit
TTGAGTTAACGCTGCGGAAATGGACAGCAGGCATCTCCAAAGTGACCTGT7600.10270075971535673No Hit
TCGAGATTGGGAATAGGTAATAAAGAAAATGAATCCTGAGGGCTGATAAA7540.1018899642439197No Hit
TTGAGTCAACACTGGGACAATTGACAAAACATCGGAAGAAATAACTTCTG7510.10148456650820119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCCG650.0143.995748
CGCCTTA19450.0141.053453
TTGAGTG152750.0138.395721
TTGAGTT369500.0138.011281
CCTTACA19800.0137.823435
GTTAACG287200.0136.609655
TTAACGC285150.0136.5226
AGTTAAC297650.0136.273744
CCGCCTT20150.0136.162542
TGAGTTA327150.0135.94652
GAGTTAA313000.0135.894233
GCCTTAC20150.0135.795974
AACGCTG189550.0134.651788
TAACGCT288150.0134.376047
GAGTGGC124100.0133.861023
ACGCTGC75500.0133.810179
TGAGTGG126750.0133.627872
TTGAGTA27200.0133.461931
CTTACAC20450.0133.081636
AACGCTC40450.0132.782238
Produced by FastQC (version 0.11.2)