FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_310_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_310_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3381506
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC57020.16862309278765142No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA53010.15676447121489656No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG47780.1412979897122761No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC43960.13000124796466425No Hit
GTAGAAACACGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC43060.12733971195082902No Hit
AAATTACACAATCTCAGTTATTTCTTTATAGCAACGCAAAAAATGGATGA37150.10986229212664417No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA81300.0111.468871
TAGTAAC85250.0105.861172
GTAACAA101200.087.596534
AGTAACA108250.084.2274553
CGCACTT25300.082.820315
AGCACGC26400.082.134591
GCACGCA26650.081.618712
TAACAAG110650.079.32625
AAGAGCG17000.076.243159
ACAAGAG120400.072.2989657
CAAGAGC124200.068.695628
AAACACG72050.065.359415
ACGCACT36050.064.721584
AACAAGA136200.064.018546
AACACGA50950.060.766326
GAAACAC79750.058.874374
ACGAGCC29350.057.1643269
AACACGT32100.056.299876
ACACGAG55500.055.9134867
CACGCAC39300.055.7094543
Produced by FastQC (version 0.11.2)