FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_302_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_302_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592781
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTCCTGTTTGGAGGACAGCTGCTCCCACATCTGGGGGCCTCTGGAT273054.606254249039695No Hit
TTGAGTCTCTGCTTGTGATTCTTTGGCTTTGTATCTAGGAGTGGAAGCGC109541.84789998329906No Hit
TTAAGATTATCTCAGGCCCTGAACTTTTCAATTCACAAGTGAAATTCTAT102391.7272820822529737No Hit
TTGAGTCTTTGTGTCCAAAGCAAGAACTACTTCTGGATTTTTAGTCAAAT98411.660140928943404No Hit
TTAAGATTCGAGAAGGCAGAATAATAAAAGAAATATTTCCAACAGGGAGA74811.26201750730877No Hit
TTGAGCCTTTGTGTCCAAAGCAAGAACTACTTCTGGATTTTTAGTCAAAT71921.2132642577950372No Hit
TTGAGTCCCCAATGAGAAGGGAAAATGAGAACAAGGGAGAGATTTTTTTC61081.0303973980272647No Hit
TTGAGTCCAATAGCTGCCCCAGCTTACCACATTAATTAGTCTTCAGCCGA54040.9116351569972722No Hit
TTGAGTGGCTGGCTGGAGACTGGAACATATACACTGGCATACAATTATAT52270.8817759003746746No Hit
TTGAGTGTTATTATCACTATATGTGTCAGCTTCATTGTCATACTTACTAT45170.7620014811540856No Hit
TCAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCT44790.7555910192803076No Hit
TTAAGCTGCTGGATCTGTGGGGAAAACAAACATCTGAGGTCCAGAATTCG41350.6975594696861067No Hit
TTGAGAAAATACCATGCACAAGATTATTAAATCTTTCATGTCCTCCTTCT35670.6017399343096353No Hit
TCAAGCATGTATGCCAGTACAATGGGAGTTATCATCGTTCCTTTCAATTT34860.5880755287365823No Hit
TTGAGTCCAGTCCTGGTTCTATCTCTTAATACCTGTACCGTTTTGTACAA34850.5879068323714829No Hit
TTAAGGAATCTGCATTCACTAACCCCTGAAGCTGGGCCATCAGTTATCAT33280.5614215030508738No Hit
TTGAGTTTATCTGAATTTTTCTCGTGTCTTCCTGCTCCTCCTTCCTTGTA31140.5253204809195976No Hit
TCAAGTAACCTTGTGGATGAGGGCTGGCAGGGCCTCTGCCTTTCAAACAT30840.5202595899666149No Hit
TTGAGTACTTGCAACAGATGGGATATGACTCACAGAATTGAAAATATTTA30040.5067638807586613No Hit
TTGAGTTCCCTTAAAAATAAATTAGAGAACAAAATTAAGTCAGAAAAAGC28850.4866890133118302No Hit
TTGAGTACTATGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAA24630.41549914723987447No Hit
TCAAGGCATTTTGCTTTTTGACTTTCTGGAGGGTGAAAGAATGATAACAT18530.3125943645292275No Hit
TTGAGGATTTCACTGGAACAGCTGTGTTTGACCTCCTCTTACAATTATTT17200.29015774797100446No Hit
TTAAGGCATTTTGCCTGTTGACTTCCTGGAAGGTCAAAGAATGGTAATAT16660.28104814425563573No Hit
TTGAGTACTTTTCTAGACATTCTTTGGCTAATGTCTTTGGTTTTTTGGAC16340.2756498605724543No Hit
TTGAGCATCCCAAAATGTTGCCATGATCTTTGCTCTTGACTGGTCCGCAT16320.2753124678422554No Hit
TTGAGCCCCCAATGAGAAGGGAAAATGAGAACAAGGGAGAGATTTTTTTC15870.2677211314127815No Hit
TTAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC15270.2575993495068162No Hit
TTAAGCATCCCAAAAAACTGTTGCCATGACCTTTGCTCTTGGCCAGGTCA14900.25135758399813757No Hit
TTAAGTGGTTTGAAGAACAGTAGGGAAGAGAACTATGGCTTGCCTTCCCT14570.2457906039498567No Hit
TTGAGTGCTATATCCACAATATATAAAGAACTTCTACAAGTCAACAACAC14430.24342885483846477No Hit
TGAAGATGTTCCCCTTTAAAAGCCCACTCTGGTCTCTGCTTGAAAGACAA13870.2339818583928972No Hit
TCAAGACACTAGGATGTTCCAAGGAGTGAGGGGTGAAATAAATTTTCTTA13850.23364446566269836No Hit
TTGAGTGCTATATGGACCAATAGGAGATACTGAAGGTTTCGAAAAAGTCT13730.23162010928150534No Hit
TTGAGTGACTAATAGACACATCACAATAACTACACCTAGAACTAACCTGA13730.23162010928150534No Hit
TTAAGTTCCATCAAGTTCAAGACACTTTTATAAGTGGTACTACCAGCCAC12880.21728091824805448No Hit
TTGAGGAACAATGCTACGCTAAGTGTTGCAACCTTTTTGAGGCCTGTTTT12470.21036436727897823No Hit
TCAAGGCTCTTTGTTGCGGCCGGGCGCCGTGGCTCACGCCTATAATCCCA12060.20344781630990197No Hit
TCAAGACTTTCTAGAGGGCCCAAAACAAAAAAACCCCAATAACACATGCT11920.20108606719851005No Hit
TTGAGTTCTCTACATCTGCTGGAATCAGGACCAGGCCATCTCTAGGCAGT11850.19990519264281412No Hit
TTGAGTGCTATATCCGGGTTATTAGTAGAAACACGAGCCAAAATAAAACT11500.19400081986433437No Hit
TTGAGTGCTATATCGTCACTAATTGTCAGGGAAATGCAAATATAAAACAC11440.19298864167373786No Hit
TTGAGTACTCAAATAAGGGAAAACAATATGTCTCTGACATCACAGGATGT11100.18725296526035753No Hit
TTGAGGGATGGGGACAGGTTTCCAGACCTCTGGCTAGTATATGGGCTCAC10950.18472251978386622No Hit
TTGAGTGCTATATGGAGGGCAGTTGAATAATTTTAAAAAGGGGAATTACA10700.1805051106563807No Hit
TTGAGCATCGTAGCCAGGCCCTGACTCTACAAAAAAGTGAAATAATTAGC10590.17864945064028706No Hit
TTGAGCGGCTCCAACATCTCCCACCAGTGCCGGCATTCCTGGGCAGGTGA10300.17375725605240383No Hit
TTGAGAGGGAATTAGTTGCCAGGAGAAGGTTCCTGCCCGTGGCAGGAGCA10190.17190159603631022No Hit
TTGAGTGCTATATGTGGAGAGATGAAGGAAAAGAGAGAGCCAAATAGATT9320.15722501227266056No Hit
TTGAGATGAGGATGAGGAGTTCCTGAATGATTGGGGCAACTTGTTGCCAT9220.15553804862166637No Hit
TCAAGCATGGTTCCTGGGGCAGCTACAGCAACATCACATGGGCTCTATCC8800.14845280128749067No Hit
TTAAGTTAAAAAAATAAAGTTCACATTTTTTATGTTCATTATGTAATTCA8720.1471032303666953No Hit
TTGAGTACTCTTCTTATGGGCAAAAGGTGAGATGGATACCCTTAGCAATC8710.14693453400159587No Hit
TTGAGTGCCAAGTCGTTTTCCCACCATCATGTACCATTTCTATCCCAATA8600.14507887398550223No Hit
TCAAGAATCATGTTCCTCTGATCAGTATTTAGTATAGATCTGTTCCTTTT8510.14356060669960743No Hit
TTGAGTCCTTAGGATGTTTTTTGCATAGGAATGGTACGTGTCAGTATATG8460.14271712487411034No Hit
TTGAGAAATGCCATCCTGTACTAAGTCACCATTGCCTGTTGCCTCTACTA8350.1408614648580167No Hit
TTGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA8060.13596927027013347No Hit
TCAAGAAACTACTGATGGGTGTGTTCTGCCTTAACAGGGATATAAAGAAA7970.1344510029842387No Hit
TTAAGAGCACACAAGAAGCTATAAACAAGATAACAAAAAATCTCAACTCT7420.12517270290377053No Hit
TTGAGTTCAATTATCTGTCTAAAGATACAATCTTCAAATGGCAGCACCCT7400.12483531017357169No Hit
TTAAGCCCTAACACATAGGTAATTAGATTAAACATAAATGGTTCTGAAAT7390.12466661380847227No Hit
TCGAGATTGGGAATAGGTAATAAAGAAAATGAATCCTGAGGGCTGATAAA7210.12163007923668269No Hit
TTGAGGGTCCCACCACCCCTGTCAATCAGTTGGCTGCAGACTCCCGTGTC7000.11808745556959484No Hit
TTGAGAACTGGCAGAGGGAGGAAGTGCGTGCGCGGTTCCACCTGATGACG6810.11488222463270584No Hit
TTAAGAAAGCACTTAGGCCTCTCCGAGACCGTTTTTCTCTTCTGTAAAAT6790.11454483190250699No Hit
TGAGTCCTGTTTGGAGGACAGCTGCTCCCACATCTGGGGGCCTCTGGATC6770.11420743917230816No Hit
TTGAGTTCCATCAAGTTCAAGACACTTTTATAAGTGGTACTACCAGCCAC6710.11319526098171162No Hit
TTGATGCCTCCACTCCCTTTGTCCCCATCAGATTCGCAAGGCCCTGTTAT6270.10577262091733708No Hit
TTGAGTACCACCTTCCAATCTTGGATGCCATTCTTTGATGAACAAATCCT6140.10357956817104463No Hit
TTGAGTGCTATATCCAGGGAAGAAGCTAAAGCTGACTAATAAAGAACTCA5950.1003743372341556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCCT20100.0138.676741
CGCCTTA20050.0137.562423
GATTCGA9550.0137.22935
TTGAGTC101600.0137.104431
CCGCCTT20300.0136.589342
ATTCGAG9600.0136.514566
TTGAGTG216800.0134.35091
AGTGCTA173100.0131.660484
GTGCTAT171450.0131.625585
TGCTATA170300.0131.58426
CGAGAAG10200.0131.330289
GCTATAT170550.0131.137997
TGAGTGC183000.0129.911182
CGAGATT1000.0129.63572
TTACACT20950.0128.204597
GCCTTAC21450.0127.901764
TGAGTCC64850.0127.603372
TTGAGTT34400.0127.3016051
CCTTACA21350.0126.477195
GAGTGCT182450.0126.344763
Produced by FastQC (version 0.11.2)