FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_302_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_302_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2815698
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC61380.2179921284171811No Hit
AAGCAGAGCAGACTGCAGGAAGTCACGGCACCGGCCACAGAAGGGGGAAA55760.1980326015076901No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA53850.19124920357225808No Hit
GTAGAAACACGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC53460.18986411184722224No Hit
GCGGTGCAGACTGCAGGAAGTCACGGCACCGGCCACAGAAGGGGGAAAAG48800.17331404149166565No Hit
AGCACAGCCACTATGCTTGATTAGCATTATAGAGATGGTACAATGATGGT47310.16802228079857998No Hit
CAACAGCACTGGGTCCATGGGGCTCCGCTTCCTTCCCATGGCCTCCCCTC46870.16645961321136002No Hit
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG41290.1466421469916163No Hit
AAATTACACAATCTCAGTTATTTCTTTATAGCAACGCAAAAAATGGATGA39200.1392194759523216No Hit
AGCACAGCCACTATGCTTGATTAGCATTATAGAGATGGTACGATGATGGT37660.13375013939705183No Hit
AAATATAGTCATAACATTGTGCAGCCGTCCCTACCATCCATCTCCATAAT34470.12242079938970729No Hit
CACGAGCACTGGGTCCATGGGGCTCCGCTTCCTTCCCATGGCCTCCCCTC31930.11339994559075584No Hit
AAACAACAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAATCCTG30010.10658103248288701No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA75600.0114.896651
TAGTAAC76850.0113.573992
GTAACAA86850.099.911214
CGCACTT24900.097.158975
AGCACGC24500.097.012951
GCACGCA25350.094.030812
TAACAAG95350.090.6157155
AGTAACA97950.088.956543
ACGCACT31050.082.099154
CACGCAC29700.080.254353
AAACACG54800.076.337615
ACAAGAG116900.073.786617
AACACGT19000.072.0004656
GCGGTGC26400.071.7519451
GTCTCGC9350.071.63961
CGGTGCA17600.071.196182
GAAACAC59200.070.672654
CCACTAT45300.070.093178
CACTATG45500.069.628069
CAAGAGC121500.069.511568
Produced by FastQC (version 0.11.2)