FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_297_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_297_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930264
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGGCTTGTATTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAA234642.5222947464375705No Hit
TCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGTCTCTTAATTCCT221202.3778196296965164No Hit
TTGAGGAACAATGCTACGCTAAGTGTTGCAACCTTTTTGAGGCCTGTTTT144261.5507425849006302No Hit
TCAAGATGATGAATGACTCAATGGCTAAGAAAACCAGTGGAAATGCTTTC134471.4455036419769012No Hit
TTAAGATTCGAGAGGGCAGAATAATAAAAGAAATATTTCCAACAGGAAGA123071.3229577840269No Hit
TTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGGA122931.3214528348941805No Hit
TCAAGAATCATGTTCCTCTGATCAGTATTTAGTATAGATCTGTTCCTTTT121741.3086607672660664No Hit
TTGAGTACTCATGTGTTCTGATCACGGTGTCTATTGTGTGGCCTGTTCCC101171.08754074112295No Hit
TTAAGATCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC89550.962629963107247No Hit
TTGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA83500.8975946613004481No Hit
TCAAGAACTGATAAGGCTCCCCATTGCTCCTTAGCTTTGGTGGTGAGAAA80520.8655607440468511No Hit
TTAAGAGCACACAAGAAGCTATAAACAAGATAACAAAAAATCTCAACTCT65900.7084010560443057No Hit
TTGAGAAAATACCATGCACAAGATTATTAAATCTTTCATGTCCTCCTTCT62570.6726047659589106No Hit
TTAAGGAATCTGCATTCACTAACCCCTGAAGCTGGGCCATCAGTTATCAT61980.6662624803281649No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATAAGA50490.5427491550785584No Hit
TTGAGTCCTATGCCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTT49760.5349019203150933No Hit
TCGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG49530.5324295038827688No Hit
TTGAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAA47130.5066303758932948No Hit
TCAAGGGGAGGTCAATGTGACTGGTGTGATACCACTAACAACAACACCAA33330.3582853899538196No Hit
TTGAGTCCTATGCTGGGTCCCTCTGCTGTAGACATAGGAAATGGATGCTT29850.3208766543690823No Hit
TCAAGTGCCAATAGATGCTCATCTTCACTGTTTATTATTCCTTCGTTGGA29480.3168992888040384No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT23300.25046653423114296No Hit
TCAAGAGCATGGAATAGAGACTCCTAAGTATCTGGCTGATTTGTTTGATT23070.2479941177988184No Hit
TCGAGCTGGATGAGAAAGTGGATGATCTCAGAGCTGACACTATAAGTTCA20880.2244524135084234No Hit
TTGAGTCCTATGCCCGGGTTATTAGTAGAAACAACAGCATTTTTTATATT20460.21993756611026546No Hit
TTGAGACCATCTGCATTTCTCGTCTCACTCCGGGCACTGAAGATCTCGCT20070.21574520781197595No Hit
GCCGCCTTACACTGACTTGAACTAAAGAGAATATCAGGAAGAGGATTCAA19880.2137027768461426No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA19470.20929542581460744No Hit
TTGAGTCCTATGCCACAGGGGAGATTCCAAAGTGGAAGGAAAAAGAATGA17810.19145102895522131No Hit
TTGAGTGTGTGTGCCTGGGTGTGTATATGAGTGTGAGTGTGCATAAGTGT17670.189946079822502No Hit
TCAAGACACTAGGATGTTCCAAGGAGTGAGGGGTGAAATAAATTTTCTTA17470.18779615249004583No Hit
TTGAGTCCTATGCATATCCCAGAAATAGTGTTGAAGTACAATCTAATGGA17180.1846787578579844No Hit
TTGAGTCTCCATAGAGGTTCTTCATTTGGGTTTTGTTATCTGAATGGAAC16430.17661653036127378No Hit
TTGAGCTAAGGACCTTTGGACCATCCATGCTATGGTTCTTGGCATCCCCT16280.17500408486193167No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA16110.17317664662934393No Hit
TTGAGTCCTATGCCACGGGGGAGATTCCAAAGTGGAAGGAAAAAGAATGA15830.1701667483639053No Hit
TTGAGTCCTATGCCCGGGATCAACCTTTCAGAAAGCACTCCTAATTAGCC15780.16962926653079125No Hit
TTGAGTCCTATGCGCAGGATGATGTCATTAAAAGGGAAAATTGAAGATGA15310.16457693729951928No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTGTAGAATTCGCCCAGTTTCAC15010.16135204630083505No Hit
TTGAGTCCTATGCGGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAA14370.1544722788369753No Hit
CAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGTCTCTTAATTCCTT14060.15113989147166826No Hit
TTGAGTACTGTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAA13510.1452275913074138No Hit
TTGAGTCCTATGCGGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATT12630.1357679110446067No Hit
TTGAGTACCATACATTTGTGCAGAAGGAGAAGACCAAATTACTGTTTGGG12520.1345854510117558No Hit
TTGAGTCCTATGCCCACCCCATGGACTCAACTGTCATTCTTTGTCTCTTA12430.13361798371215053No Hit
TTGAGTCCTATGCAAAAGCCATAGGAAATTGCCCAATATGGGTGAAAACA12100.13007060361359785No Hit
TTGAGTCCTATGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTCCATGAA11440.12297584341649252No Hit
TTGAGTCCTATGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCT11440.12297584341649252No Hit
TTGAGTCCTATGCGGGAGGATGATGTCATTAAAAGGGAAAATTGAAGATG11350.12200837611688725No Hit
TTGAGTCCTATGCAAGGGTTTCCATGTTCCAGCAAAGGAACAGGTAGAAG10990.11813850691846615No Hit
TTGAGTACTCAAATAAGGGAAAACAATATGTCTCTGACATCACAGGATGT10830.11641856505250123No Hit
GGGGGGAGCAGAAGCACAGCTAATGATAACATATGATACACCAAAGATGT10400.11179622128772047No Hit
TTGAGTCCTATGCGGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAA9840.10577642475684322No Hit
TTGAGTCCTATGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCTTACT9720.10448646835736952No Hit
TTGAGTAGCTGTTTTGACCACATGAGGTGAGTTTGAAGATGTTATCCCAG9450.10158406645855371No Hit
TGAGGCTTGTATTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAAA9450.10158406645855371No Hit
TTGAGTCCTATGCTGGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATA9330.10029411005908001No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTACTTTCT9330.10029411005908001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCAG202.2047989E-6144.00519
TATACCG202.2047989E-6144.00519
CGATAAG100.0072382498144.00519
TTGAGTT30450.0135.314211
TCAAGGC30450.0135.314211
TTGAGTC417150.0132.670171
GCCGCCT29100.0132.185241
CCGCCTT29300.0131.023012
GAGTCCT398850.0130.786653
CGCCTTA29600.0129.910463
GTCCTAT386450.0128.943685
CTATCCG900.0127.997658
TCGAGTA6200.0127.8014141
ATTCGAG15550.0127.3288656
CGAGAGG15500.0127.281949
TGAGTTA9700.0126.230752
TCCTATG390200.0126.209896
TATGCCG32800.0125.784949
CCTATGC386350.0125.7158367
TCCTACG2350.0125.614726
Produced by FastQC (version 0.11.2)