FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_297_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_297_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863562
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC155160.5418426421359133No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT92260.32218614438940035No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA69260.2418665983135689No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA59870.20907527058956643No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA57640.20128776677438798No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT56650.19783053413895002No Hit
CAACAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG47930.16737894971367828No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATG44390.15501672392635465No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA40890.14279418430611945No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATGT40250.140559205632705No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC39850.13916234396182098No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT38770.1353908174504341No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACT37560.13116531089600994No Hit
ATATAAATCCTTACTTTCTCTTCATAGATGTACCCATACAGGCAGCAATT37230.1300129000175306No Hit
ATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC36560.12767315671879986No Hit
AGCACAGCATTTTCTTGTGAACTTCAAGTACCAGTAAAAGAACTGAAAAT35710.12470482566817132No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG35350.12344765016437569No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC34020.11880308510868631No Hit
CCCATACACTGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA33370.11653318489349977No Hit
GCACAGCATTTTCTTGTGAACTTCAAGTACCAGTAAAAGAACTGAAAATC33340.11642842026818347No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC32700.11419344159476905No Hit
ACAACAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAA32500.11349501075932703No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT32300.112796579923885No Hit
AGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATTGTTAAAACAA32280.11272673684034079No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT31660.11056160125047056No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT31160.10881552416186553No Hit
ATCCAATGATGACCAATAACCCCATATACATCTTCGGAGCTTATGTGTAC30980.10818693640996774No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT30740.10734881940743732No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT30480.10644085932136269No Hit
GCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAATTGTTAAAACAAC29580.10329792056187363No Hit
CTTCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACA28970.1011677065137755No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC13500.0110.450551
GCACGCA14000.0107.521782
CACGCAC14100.0107.253173
CGGTGCA1800.0100.0335542
GTAACAA63350.099.801044
GAAACAC23300.095.8060464
CGCACTT16700.093.132595
ACAGCGT2050.091.328574
ACGCACT17250.090.585324
AAACACG25700.086.294285
TAGTAAC76700.082.165762
ACGTGCA10650.081.830262
GTAGTAA78500.081.576191
TAACAAG78500.080.535865
ACAAGAG79800.079.580637
AGTAACA84000.075.527753
GCGGTGC15200.070.611271
CACAGCG2800.069.440953
GAAGCGG7900.069.297541
AACACGT5500.066.7650456
Produced by FastQC (version 0.11.2)