FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_284_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_284_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657503
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC372645.66750265778255No Hit
TTGAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAA180862.7507098826925502No Hit
TCAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCT165602.5186196869063715No Hit
TTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGGA161292.4530686552000525No Hit
TTGAGGTCTGGAAATTTAGTTTCTAACAAGTTTGTTTGTTGAAAAGGGCA141432.151016801444252No Hit
TTGAGTCTCCATAGAGGTTCTTCATTTGGGTTTTGTTATCTGAATGAAAC129471.9691164907232364No Hit
TTAAGAGCACACAAGAAGCTATAAACAAGATAACAAAAAATCTCAACTCT119031.810333945244356No Hit
TTGAGAAAATACCATGCACAAGATTATTAAATCTTTCATGTCCTCCTTCT116341.7694215843882082No Hit
TTGAGAACAAAACAATTTTATGAATTCAGAAATGGAAATAATAGTTTGCT104561.5902589037616557No Hit
TTGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA90381.3745944885422576No Hit
TTGAGTTAGACATCGTTCGGGAGTACCACCTTCCAATCTTGGATGCCATT84001.2775607107496088No Hit
TTGAGTTCATCCATGGCACCACTTAGCCTTTGAAGATTCTTTACTTCTAG76271.159994707248484No Hit
TTGAGTTGGACATGATGCAAAAACCTGGGAAAACAGGAACAATTGTCTAT64580.9822008416691634No Hit
TTAAGATTCGAGAAGGCAGAATAATAAAAGAAATATTTCCAACAGGAAGA64410.9796153021355036No Hit
TCAAGTGCCAATAGATGCTCATCTTCACTGTTTATTATTCCTTCGTTGGA49970.7599965323352137No Hit
TTGAGTAGAAGTAACGATCTCTTTGATCAATGGGGGTATGAGGAATCACC44360.6746737277244362No Hit
TCGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG43860.66706919968426No Hit
TCAAGGAAAATGAAACATCTGTTCTACTGGGCTAACTCGGTTCTGAGTAA38240.581594304512679No Hit
TTAAGGAATCTGCATTCACTAACCCCTGAAGCTGGGCCATCAGTTATCAT33170.5044843901852919No Hit
TTGAGTTGGACATGCAAAAGCCATAGGAAATTGCCCAATATGGGTGAAAA28350.43117673987799293No Hit
TTGAGTTGGACATATACACTGGCATACAATTATATCAGTCCCTAGGAAGG27740.421899215668978No Hit
TTGAGTGGCTGGCTGGAGACTGGAACATATACACTGGCATACAATTATAT26540.403648348372555No Hit
TTGAGTTGGACATTGCCCAATATGGGTGAAAACACCTTTGAAGCTTGCCA25000.38022640200881214No Hit
TCGAGCTGGATGAGAAAGTGGATGATCTCAGAGCTGACACTATAAGTTCA21730.33049278862605946No Hit
TTGAGTTGGACATGATGCCAAAACCTGGGAAAACAGGAACAATTGTCTAT21490.32684261516677493No Hit
TTGAGTAGAGACAGTTGACTCAGGGCCTTTGAGAACACGCAACAATGAAT21270.32349662282909736No Hit
TTGAGTTGGACATGATGCGAAAACCTGGGAAAACAGGAACAATTGTCTAT20960.3187818154441881No Hit
TTGAGTTGAAGAAATTGGTGGCAAATTCTGCCTCCTATAATATTACACTT20750.31558791366731404No Hit
TCAAGACACTAGGATGTTCCAAGGAGTGAGGGGTGAAATAAATTTTCTTA20460.31117728740401185No Hit
TTGAGTTGGACATGGTGCAAAAACCTGGGAAAACAGGAACAATTGTCTAT20220.30752711394472726No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTGTAGAATTCGCCCAGTTTCAC20210.3073750233839237No Hit
TTGAGTTTGACATCTCTGGCATTCTTTTTCTTCTGGAACTCGGTTTTCTT19190.2918617861819642No Hit
TTGAGTTGGACATGGCTGGAGACTGGAACATATACACTGGCATACAATTA19140.2911013333779465No Hit
TTGAGTTGGACATGCAAGAGCCATAGGAAATTGCCCAATATGGGTGAAAA18840.28653861655384083No Hit
TCAAGGGGAGGTCAATGTGACTGGTGTGATACCACTAACAACAACACCAA18360.27923826963527165No Hit
TTGAGTTGGACATGGAGACTGGAACATATACACTGGCATACAATTATATC18190.2766527301016117No Hit
TTGAGTTGGACATACGGTGGATTAAACAAAAGCAAGCCTTACTACACAGG16900.257033047757957No Hit
TTGAGTTGGACATGATGCTAAAACCTGGGAAAACAGGAACAATTGTCTAT16780.2552079610283147No Hit
TTGAGTTGGACATGCAGAAGCCATAGGAAATTGCCCAATATGGGTGAAAA16770.25505587046751116No Hit
TTGAGTTTTGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCAT16590.25231824037304773No Hit
TTGAGTGAGACGAGAAATGCAGATGGTCTCAGCTATGAACACAGCAAAAA15610.23741336541430227No Hit
TTGAGTTGGACATGCCTGAAGGAGAGATTATCTTCTATGTATGTGCTAGC15580.2369570937318917No Hit
TTGAGTCGCACCCATGAGACAAAACTCATATTCTTTTCTGCTGATCCCAA14660.22296476213796743No Hit
TTGAGTGGGCTTTCTGAAGATTACAGCAGCACTCTGACTTCCATGGCCCT14450.21977086036109342No Hit
TTGAGTTGGACATTTAATAACTCACTCTCCAAAGAGTTGCTGTTGTGTAA13580.20653898157118675No Hit
TTGAGTAACCCATCCCCGGAAAGAGCAACTACAAGCAGTGAAGATGATGT13340.20288880811190216No Hit
TTGAGTTGGACATCCGGGTGCTTCCCTATAATGCACGACAGAACAAAAAT13280.201976264747081No Hit
TTGAGTTGGACATGATGGAAAAACCTGGGAAAACAGGAACAATTGTCTAT13050.19847818184859992No Hit
TTGAGTTGGACATATGCACTGGCATACAATTATATCAGTCCCTAGGAAGG12770.19421964614610124No Hit
TCAAGTACAGAATATAGATACAGCTTAAATTTTGTCTTACAAAGTCAGCA12720.1934591933420836No Hit
TTGAGACTAGTAAGCAGCCCATAAGTCAGAAGCCAGTATATCAGAGTGGC12590.19148201605163778No Hit
TTGAGTTGGACATGCTGGGTCCCTCTGCTGTAGACATAGGAAATGGATGC12440.1892006576395849No Hit
TCAAGAGATACAGTGGTGGAAAAATATATCCCTATACTCATGAAGCTTAC12440.1892006576395849No Hit
TTGAGTTGGACATGCATAAGCCATAGGAAATTGCCCAATATGGGTGAAAA12070.18357330688985452No Hit
TTGAGTTGGACATATACGCTGGCATACAATTATATCAGTCCCTAGGAAGG12010.18266076352503335No Hit
TTGAGTTGGACATAGGAAATTGCCCAATATGGGTGAAAACACCTTTGAAG11810.17961895230896285No Hit
TCAAGAATCATGTTCCTCTGATCAGTATTTAGTATAGATCTGTTCCTTTT11270.17140606202557251No Hit
TTGAGTTGGACATTTGCCCAATATGGGTGAAAACACCTTTGAAGCTTGCC11020.1676037980054844No Hit
TTGAGTACTCCCCCCTGGAACCTCATTTGCTTAATTAGCAATTTATCATT10690.16258480949896806No Hit
TTGAGTTGGACATGCAAAGGCCATAGGAAATTGCCCAATATGGGTGAAAA10680.16243271893816455No Hit
TTGAGTTGGACATTGGGGATGTGGAATATTGAATTTCCTTTGTTTATAAA10600.16121599445173634No Hit
TTGAGTTGGACATGCAACAGCCATAGGAAATTGCCCAATATGGGTGAAAA10570.16075972276932576No Hit
TTGAGTTGGACATATGGGTGAAAACACCTTTGAAGCTTGCCAATGGAACC10540.1603034510869152No Hit
TTGAGTTGGACATGCAAACGCCATAGGAAATTGCCCAATATGGGTGAAAA10470.1592388171612905No Hit
TTGAGTTGGACATGCTAAAGCCATAGGAAATTGCCCAATATGGGTGAAAA10380.1578700021140588No Hit
TTGAGTGCCAAGTCGTTTTCCCACCATCATGTACCATTTCTATCCCAATA10320.15695745874923764No Hit
TTGAGTTGGACATGCTGGAGACTGGAACATATACACTGGCATACAATTAT10300.1566532776276306No Hit
TTGAGTTGGACATGTTGCAAAAACCTGGGAAAACAGGAACAATTGTCTAT10150.15437191921557772No Hit
TTGAGTTACAATTCAAACAAAATTCTCACTACCCCAATTCTGATAACTTT10120.15391564753316714No Hit
TTGAGTTGGACATTGCCCCATATGGGTGAAAACACCTTTGAAGCTTGCCA10030.15254683248593542No Hit
TTGAGTTGGACATGCAATAGCCATAGGAAATTGCCCAATATGGGTGAAAA9880.15026547407388255No Hit
TTGAGTTGGACATGCAAGCCATAGGAAATTGCCCAATATGGGTGAAAACA9860.1499612929522755No Hit
TTGAGTTGGACATTGCCCGATATGGGTGAAAACACCTTTGAAGCTTGCCA9690.14737575341861558No Hit
TTGAGTTGGACATGCGAAAGCCATAGGAAATTGCCCAATATGGGTGAAAA9500.14448603276334862No Hit
TTGAGTTGGACATTGGTGGCAAATTCTGCCTCCTATAATATTACACTTTA9470.14402976108093804No Hit
TTGAGTTGGACATGGTCACAGTGATTGAGTTGAAGAAATTGGTGGCAAAT9280.14114004042567105No Hit
TCAAGCATGTATGCCAGTACAATGGGAGTTATCATCGTTCCTTTCAATTT9140.1390107725744217No Hit
TTGAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAACAATAAAA9000.13688150472317237No Hit
TGAAGGAGAGATTATCTTCTATGTATGTGCTAGCAGTAGCTCTGTTTCCA8950.13612105191915475No Hit
TTGAGTTGGACATGCAAATGCCATAGGAAATTGCCCAATATGGGTGAAAA8610.1309499728518349No Hit
TCAAGTCTTCACAAGCGCTGATCATTTTTACCACCTGTAAGATACCTCTT8570.1303416106086208No Hit
CAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCTG8510.12942906724379966No Hit
TTGAGTTGGACATGCGCGAGTGGCAGGAGCAAAGTAATAAAAGGGTCCTT8210.1248663504196939No Hit
TTGAGTAGCTGTTTTGACCACATGAGGTGAGTTTGAAGATGTTATCCCAG8050.1224329014468375No Hit
TTGAGTTGGACATGCCAAAGCCATAGGAAATTGCCCAATATGGGTGAAAA8000.1216724486428199No Hit
TTGAGTTGGACATGCAGCCATAGGAAATTGCCCAATATGGGTGAAAACAC7980.12136826752121284No Hit
TAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCCG7970.12121617696040932No Hit
TTGAGGAACAATGCTACGCTAAGTGTTGCAACCTTTTTGAGGCCTGTTTT7950.12091199583880226No Hit
TTGAGTTGGACATGATGCAAAAGATAAATCGCAATTATTCTTCATGTCTT7910.12030363359558816No Hit
TTGAGTTTCCTTTGATTTCTCCGAATCTATGGGGGCTAATTAGGAGTGCT7800.11863063742674938No Hit
TTGAGTTGGACATGGAAAAGCCATAGGAAATTGCCCAATATGGGTGAAAA7660.11650136957550004No Hit
TTGAGTTGGACATGATGTAAAAACCTGGGAAAACAGGAACAATTGTCTAT7650.11634927901469651No Hit
TTGAGTTGGACATGCACAAGCCATAGGAAATTGCCCAATATGGGTGAAAA7590.11543673564987536No Hit
TTGAGTTGGACATGGGAGATGATGCAAAAGATAAATCGCAATTATTCTTC7470.11361164892023307No Hit
TTGAGTTCTGGGATGTGGAAAAGAAAGGAGAAGGATGAGCAGGGCTGCGC7280.11072192826496609No Hit
TTGAGAGAAAACTAAAGAAAATGCTGGGTCTCTCTGCTGTAGACATAGGA7200.1095052037785379No Hit
TTGAGTTCGACATGCTTTATGGTCTGGCGGTTAAAGGACAATCTCATCTG7190.10935311321773437No Hit
TTGAGTTGGACATCAGGGGGAGTGTTATTATCACTATATGTGTCAGCTTC7020.10676757368407444No Hit
TTGAGAGGGAATTAGTTGCCAGGAGAAGGTTCCTGCCCGTGGCAGGAGCA6950.10570293975844978No Hit
TTGAGTTGGACATACACTGGCATACAATTATATCAGTCCCTAGGAAGGAG6830.10387785302880748No Hit
GCCGCCTTACACTGACTTGAGTTCATCCATGGCACCACTTAGCCTTTGAA6690.10174858517755814No Hit
TTGAGTTGGACATGGGAGCAGCTCTGATGTCCATCAAGCTCCAGTTTTGG6660.10129231349514756No Hit
TTGAGTTGGACATGGTCGCAGTGATTGAGTTGAAGAAATTGGTGGCAAAT6590.10022767956952287No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGAC151.2636094E-4144.016594
AGTGCGA100.007236047144.016574
AGTTCGC100.007236047144.016574
AGTCGGC100.007236047144.016574
GGACACG202.2042732E-6144.005628
TTCGACC151.2639923E-4144.005626
GCCGCCT21300.0141.671661
CCGCCTT21600.0140.693562
CGCCTTA21400.0140.651723
TTAAGGT43400.0140.55371
TCAAGTG23750.0139.186661
CTTACAC21300.0138.935016
AGTCGGA1350.0138.682634
GTCGGAC1200.0138.015885
TAAGGTC44250.0137.843292
AGTTAGA16850.0137.606344
GTCTGGA17100.0136.426386
GTTAGAC16550.0136.184865
GAGTTAG18000.0136.015663
TTGAGTT309300.0136.005311
Produced by FastQC (version 0.11.2)