FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n02_284_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n02_284_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3700247
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC47650.12877518716993758No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA46870.12666721978289558No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG39940.10793874030571474No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA37550.10147971203003477No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT37050.10012845088449501No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG37700.088.04945
GAAACAC40700.081.5658954
AACACGA23350.079.863886
ACACGAG24050.077.838747
ACGTGCG851.8189894E-1276.2423559
AACACGT19850.075.809446
AGCACGC12350.068.829661
CACGAGC27200.068.8243268
ACACGTG20950.066.6738057
ACGAGCC10900.064.079329
ACGAGCG1350.064.005939
ACAACGC11200.057.2149478
TAGTAAC39050.056.9953352
GTAGTAA40400.056.5247271
TAGAAAC83700.055.6776242
GTAACAA39450.054.031474
GCACGCA16650.051.0469322
GCGATCC6800.048.7103929
AGCGATC7350.045.0617458
ACGCACT19800.043.2796634
Produced by FastQC (version 0.11.2)