FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_smart_pool1_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_smart_pool1_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16224319
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCC6402863.9464583998872307No Hit
TTAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC4722532.910772402835521No Hit
TTGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA2899911.787384727827405No Hit
TCGAGGGTCATGTCAGAAAGGTAGCGCGAAGTAGGTACAGATGCAATTGT1909351.1768444641651832No Hit
TCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAGC1853181.1422235965651317No Hit
TTGAGTTATCCGGCCCCAAGTGGCGTTGTCAAGTCTCATTTTTCTGAGAA1537220.9474789049697556No Hit
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAAC1504900.9275581921188804No Hit
TTAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAACTCCAGATCCATCCT1198720.738841488508701No Hit
TCAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCT1191490.7343852151822212No Hit
TTGAGTCTCGTTGTCAGAGTGGAACCCCCAAACGGTAATTTGGTCTTCTC1140030.7026673970106234No Hit
TTGAGTGGCTCTGGGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCA1106570.6820440352534982No Hit
TCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGC1082550.667239099527074No Hit
TTAAGAGCACACAAGAAGCTATAAACAAGATAACAAAAAATCTCAACTCT1044320.6436757068201137No Hit
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACT1021660.6297090189116721No Hit
TTGAGTTAACCAGTAGACCAATATAACATAATAAGAAAATTCAATACATC954370.5882342426822352No Hit
TCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG893460.5506918348930393No Hit
TCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGG870490.5365340757907928No Hit
TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTT839730.5175748824958385No Hit
TCAAGGGGAGGTCAATGTGACCGGTGTAATACCACTGACAACAACACCCA837880.5164346189199066No Hit
TTGAGTCCCCATAGAGCTTTGCCATTTGAGTCTCGTTGTCAGAGTGGAAC753310.4643091645325761No Hit
TCAAGTCAAGGCTTTTCTATGGAATAAGGAATGGACAGCAGGGGGCTGTT708500.4366901316474362No Hit
TCGAGGAAATGTCACGAGACTGGTTCATGCTCATGCCTAGGCAAAAGATA679490.41880956605944447No Hit
TTGAGAAAATACCATGCACAAGATTATTAAATCTTTCATGTCCTCCTTCT650230.4007749107990295No Hit
TTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGGA647400.39903061570719855No Hit
TTGAGGTCACACAGCTGCTTCCAGGGCCTCAGATTTTAAAACCAGCATCC617480.3805891637115863No Hit
TCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCTTGGG580440.3577592378453604No Hit
TCAAGATGATGAATGACTCAATGGCTAAGAAAACCAGTGGAAATGCTTTC550270.3391636961773249No Hit
TCAAGAATCATGTTCCTCTGATCAGTATTTAGTATAGATCTGTTCCTTTT547830.3376597809744742No Hit
TCAAGCTCACTCTCCGCCGCCCCTAAAGACCTCTTCCTCCCTCCTCTGAA540350.3330494179755711No Hit
TCCAGGAGGACCCTACAAAATTGGAACCTCAGGGTCTTGCCCTAACGTTA534820.32964095442156927No Hit
TTGAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAA506970.3124753649136213No Hit
TTGAGTTACACGTGGGTGCTTTCCTATAATGCACGATAGAACAAAAATTA476890.2939352955276582No Hit
TTGAGTAGATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA470770.2901631803467375No Hit
TCAAGACACTAGGATGTTCCAAGGAGTGAGGGGTGAAATAAATTTTCTTA456640.2814540320613765No Hit
TTAAGATCCTATCTGAAGTTGAAGTGTATGGGTCACACGGCCGGCTTCAA434940.26807904849504005No Hit
TTAAGATTCGAGAGGGCCGAATAATAAAAGAAATATTTCCAACAGGAAGA419740.2587103964117076No Hit
TCGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG413480.2548519910142299No Hit
TGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGAC396610.2444540199191103No Hit
TGGAGCATCTTCTGCATTGATAACGTTGTGAGTTGATAACCTGATATGTT384630.2370700428165891No Hit
TTGAGTCTCTGCTTGTGATTCTTTGGCTTTGTATCTAGGAGTGGAAGCGC373120.22997575429822356No Hit
TCAAGAACTGATAAGGCTCCCCATTGCTCCTTAGCTTTGGTGGTGAGAAA368140.22690628802355278No Hit
TCAAGCCGTCCAAATCTTTGTCTTTGTCTGATCTTTAGTCCTAATGCAGC335700.20691161212991438No Hit
TTGAGTTCATCTGGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGAC328080.2022149589144543No Hit
TCAAGAAGGAAAACTAGTAAAAGGATCCTTTGGAAAAGCACTAAGAGTAA317210.19551513995749223No Hit
TTGAGTGTTTTGGAGGGAAAAGCAGTTTGTAAATGTTTGTTGAATGACAG315290.1943317312732818No Hit
TCAAGAACCCTGCAGAAATGGACAGCAGGCATCTCCAAAGTGACCTGTGT313570.19327159432700997No Hit
TTAAGAAATCTGCATTCACTAACACCTGAAGCTGAGCCATCAGTTATCAT302220.1862759231989953No Hit
TCAAGAATCATGTTCCTCTGATCAGTGTTTAGTATAGATCTGTTTCTTTT301310.18571503679137474No Hit
TTAAGGAATCTGCATTCACTAACCCCTGAAGCTGGGCCATCAGTTATCAT300420.18516647755754803No Hit
TCAAGCATCAGGAAGAATCACAGTCTCTACCAAAAGAAGCCAACAAACTG298060.18371187104987274No Hit
TTGAGTTTCAAAGCATCCATTCCCTATCTCTACAGCAGAGGGGCCCAGCA293510.18090743901176992No Hit
TCAAGTGCCAATAGATGCTCATCTTCACTGTTTATTATTCCTTCGTTGGA292240.1801246634758599No Hit
TTAAGATTATCTCAGGCCCTGAACTTTTCAATTCACAAGTGAAATTCTAT291990.1799705738034367No Hit
TTGAGTAACCCATCCCCGGAAAGAGCAACCACAAGCAGTGAAGATGATGT275680.1699177635745451No Hit
TTGAGTGCCAAGTCTCTTTTCCACCATCATGTACCATCTCTATCCCAATA272270.16781598044269222No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTATAGAATTCGCCCAGTTTCAC260120.1603272223629232No Hit
TCAAGTACAGAATATAGATACAGCTTAAATTTTGTCTTACAAAGTCAGCA252590.1556860414295355No Hit
TTAAGATTCGAGAAGGCAGAATAATAAAGGAAATATTTCCAACAGGGAGA251580.15506351915294564No Hit
TTGAGTCAGTCGCTTGGTCAGCAAGTGCTTGTCATGATGGCATCAATTGG246800.15211732461621347No Hit
TCAAGTCTTCACAAGCGCTGATCATTTTTACCACCTGTAAGATACCTCTT230700.1421939497121574No Hit
TTAAGTGGTTTGAAGAACAGTAGGGAAGAGAACTATGGCTTGCCTTCCCT225860.13921077365404366No Hit
TTGAGGCTTGTATTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAA222060.13686861063321054No Hit
TTGAGTTAACCAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATG215780.132997878061939No Hit
TTGAGTAGAAGTAACGATCTCTTTGATCAATGGGGGTATGAGGAATCACC213200.13140767264253125No Hit
TCGAGCTGGATGAGAAAGTGGATGATCTCAGAGCTGACACTATAAGTTCA212590.13103169384181856No Hit
TCAAGGATTGGAGGTGGCAGCCCCACCTGGCAGACACCATCGTATATGCC207000.12758624876643512No Hit
TTAAGATTCGAGAGGGCAGAATAATAAAAGAAATATTTCCAACAGGAAGA204780.12621793247531685No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTGTAGAATTCGCCCAGTTTCAC195590.12055359611703886No Hit
TTGAGTTACACGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGC193450.11923458852109602No Hit
TCAAGGCCCATGGTCCGTGCTCGCTTCCAGCTCCAGCTTCCCGCTTGGGA193230.11909898960936356No Hit
TCAAGGATCTAGAACAAGAAATAACATTTGACCCAGTAATCCCATTACTG193060.11899420863211577No Hit
TAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCCG189540.1168246260443967No Hit
TCAAGGGGAGGTCAATGTGACTGGTGTGATACCACTAACAACAACACCAA184380.11364421520558121No Hit
TCGAGCATACAAAATAGGGAAAAGCACAAATCTGATAAGAGAAATACCCT180460.11122808914198494No Hit
TTGAGGTCTGGAAATTTAGTTTCTAACAAGTTTGTTTGTTGAAAAGGGCA177670.10950844839774168No Hit
TGAGTCTCGTTGTCAGAGTGGAACCCCCAAACGGTAATTTGGTCTTCTCC172390.10625407451616306No Hit
TGAGTGGCTCTGGGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCAA167120.10300586422148134No Hit
TCAAGACACTAGGATGTTCCAAGGGGTGAGAGGAGAAATAAATTTTCTTA165950.10228472455454062No Hit
TTGAGTAGCAGTTTTGACGACATGTGGTGAGTTTGACGATGTTATCCCAG164640.1014772946710429No Hit
TTGAGTGCCTATACCTGCAACTGTTTGGAAATCCAAAATTTAGACTCTGC163770.10094106261101005No Hit
TGAGTTATCCGGCCCCAAGTGGCGTTGTCAAGTCTCATTTTTCTGAGAAA162740.10030621316062634No Hit
TTGAGGTTAGATTTTTTTTATATTTTGTTTTGTGTTATTTTTTTCTTTAA162700.10028155881303862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTA1134000.0141.129413
CTTACAC1148800.0139.17986
GCCGCCT1155200.0138.453281
TTAAGGT1295350.0137.583021
CCTTACA1170150.0136.584845
TAAGGTC1326750.0134.402192
TACACTG1229100.0130.127858
TTACACT1229750.0129.982977
ACACTGA1232050.0129.413029
CCGCCTT1238300.0129.378832
CAAGACG110700.0126.961412
TCGAGGG257250.0125.802571
CGATAGT110900.0125.3072057
CGAGAGG82800.0124.092039
AAGACGA112800.0123.956793
GACGATA112950.0123.1547245
TTAAGGC179850.0122.2842251
TCAAGTG235300.0121.623571
AGACGAT114950.0121.262514
TTGAGTT2813650.0121.065751
Produced by FastQC (version 0.11.2)