FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_smart_methods_pcr_control_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_smart_methods_pcr_control_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4919
Sequences flagged as poor quality0
Sequence length150
%GC69

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT294159.78857491360032TruSeq Adapter, Index 2 (97% over 37bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1002.0329335230737953TruSeq Adapter, Index 2 (97% over 35bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT420.8538320796909941TruSeq Adapter, Index 2 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG290.5895507216914007TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATGTCGTAT170.34559869892254524TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTAGCTCGTAT160.32526936369180726TruSeq Adapter, Index 2 (97% over 37bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT150.3049400284610693TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATATCGTAT80.16263468184590363TruSeq Adapter, Index 2 (97% over 37bp)
TATATATATATATATATATATATATATATATATATATATATATATATATA70.1423053466151657No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGCGTAT60.12197601138442773TruSeq Adapter, Index 2 (97% over 37bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATATCGTAT50.10164667615368977TruSeq Adapter, Index 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAAGC100.007022275144.03
CGCCGCC100.007022275144.01
TCGGAAG3450.0141.913043
CGGAAGA3450.0141.913044
ATCGGAA3450.0141.913042
GATCGGA3350.0141.850741
AAGAGCA3550.0139.943667
GAAGAGC3550.0139.943666
GAGCACA3550.0139.943669
GGAAGAG3550.0139.943665
AGAGCAC3600.0138.08
TGCCGTC3450.028.80000150-54
CCGTCTT3450.028.80000150-54
AATGGGG304.4326727E-528.80000170-74
AAAATGG304.4326727E-528.80000170-74
GCCGTCT3450.028.80000150-54
TCTTCTG3450.028.80000155-59
AAAAATG304.4326727E-528.80000170-74
AAATGGG304.4326727E-528.80000170-74
GAAAAAA3400.028.865-69
Produced by FastQC (version 0.11.2)