FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_smart_methods_extraction_control_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_smart_methods_extraction_control_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844967
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGGTCACACAGCTGCTTCCAGGGCCTCAGATTTTAAAACCAGCATCC13175815.593271689900314No Hit
TTGAGTGCCTATACCTGCAACTGTTTGGAAATCCAAAATTTAGACTCTGC363144.297682631392705No Hit
TTGAGTTGATAACCAAAGATGAGCCTTCCTGTGCTGGGTTCCTTCATGAT302193.576352685962884No Hit
TCAAGGAACTGCTCAGAGGAGCCGTTTACAGCACAGAAGCAAGAGAAGAG233222.760107791191845No Hit
TTGAGATTCGTAAGCCAAAGGCTTTTGTTAGATTGTGAGGCAAGGTAGAG193832.293935739502253No Hit
TGAGGTCACACAGCTGCTTCCAGGGCCTCAGATTTTAAAACCAGCATCCT158071.8707239454321887No Hit
TTGAGTGCCTATATCATGAAGGAACCCAGCACAGGAAGGCTCATCTTTGG140941.667994134682183No Hit
TCAAGGGATCCCTTACCTACTCTTAGCCTCCGTTTCCTTTGCTGTAACAG109511.296026945431005No Hit
TTGAGTGCCTATACCCAGCACAGGAAGGCTCATCTTTGGTTATCAACTCA79740.9437054938240192No Hit
TTGAGTGCCTATACGTGTGCAAAAATCTCTGAAGCAAGCAGAGATATACC74660.8835848027201063No Hit
TTGAGTCCTCACCCAACCCAAACCATGCTGGCAGCCTGATCTCCAACTTC67000.7929303747957022No Hit
TTGAGTCCTATATCATGAAGGAACCCAGCACAGGAAGGCTCATCTTTGGT61280.7252354233952332No Hit
TTGAGTGCCTATAAGAAGATAAGTTTTGTATTTCCATCTAAATAGATACA58930.6974236863688168No Hit
TTGAGTGCCTATAGTGTGCAAAAATCTCTGAAGCAAGCAGAGATATACCA53550.6337525607508933No Hit
TTGAGTAAAGCCCATGTACAGTGCTCAGAACAGGGCATGGCCCATAATAA51250.6065325628101452No Hit
TTGAGTCCCTTTTCCCTTTGCTGATTCTAATCTGTATCTTTTCCCTGTAA48780.5773006519781245No Hit
TTGAGTGCCTATAGAAGATAAGTTTTGTATTTCCATCTAAATAGATACAT46230.5471219586090345No Hit
TTGAGTGCCTATATCATGCAGGAACCCAGCACAGGAAGGCTCATCTTTGG44900.5313816989302541No Hit
TTGAGTTCCAGGAAAAGTGAATTCCAGGAATTTATGTTTGGGTAGACCTA43490.5146946567144042No Hit
TTGAGTGCCTATAAACAGGACCCAAAGTGAGCCCTATATCATGAAGGAAC37900.4485382269366732No Hit
TTGAGTGCCTATACTGATCAATTTGATAACCCTTGGAAGAATCAATGTAA35010.41433570778503775No Hit
TTGAGTGCCTATAGGGGAGTGAGCTCATCCCAGAGGGAATGAGGAGAGAG30550.3615525813434134No Hit
TAGAGGTCACACAGCTGCTTCCAGGGCCTCAGATTTTAAAACCAGCATCC30510.36107919007487865No Hit
TGAGTTGATAACCAAAGATGAGCCTTCCTGTGCTGGGTTCCTTCATGATA30420.3600140597206755No Hit
TTGAGTGCCTATAGCTGCAGTATTTTCTCTGCCCTCTTTCCAGTAGGGAC30000.35504345140106064No Hit
TTGAGTTCCTTCATGATATAGGGCTCACTTTGGGTCCTGTTTGTAGCTAA28900.3420251915163551No Hit
TTGAGTGCCTATACCAAAGGGGGAAAAATACATGCATATTCTGCAGTCTT28200.333740844316997No Hit
TTGAGTTCACACAGCTGCTTCCAGGGCCTCAGATTTTAAAACCAGCATCC26250.31066301997592805No Hit
TTGAGTGCCTATACCCCGATGACAGGGCTGTGAAGAAATGTGCCTAGATA25940.3069942376447838No Hit
TTGAGTGCCTATACCCTTGGTCATGGTTCATTACAGGGAAAAGATACAGA25870.30616580292484796No Hit
TGAGTGCCTATACCTGCAACTGTTTGGAAATCCAAAATTTAGACTCTGCT25680.30391719439930787No Hit
TTGAGTCCCTGGAGAGTCTATCCAAGGTCAAGACCCACAGCCATAGATAG25660.3036804987650405No Hit
TTGAGTGCCTATACTTGGGGCAGAAGAGGTCCACAGACTAACCAAAAGAT24450.2893604128918644No Hit
TTGAGTGCCTATATGTGGGTCTTACCTGCAACTGTTTGGAAATCCAAAAT24200.2864017174635222No Hit
TTGAGTGCCTATACGTTCATTAAGGCAAGCTCACGTGTGCAAAAATCTCT24140.2856916305607201No Hit
TTGAGTCACATTCAGTTGGTGATTTACTTGGGGCAGAAGAGGTCCACAGA23680.2802476309725705No Hit
TTGAGTGCCTATAACATTCATTAAGGCAAGCTCACGTGTGCAAAAATCTC23670.28012928315543684No Hit
GCCGCCTTACACTGACTTGAGGTCACACAGCTGCTTCCAGGGCCTCAGAT23540.2785907615326989No Hit
TTGAGTCTATACCTGCAACTGTTTGGAAATCCAAAATTTAGACTCTGCTA22150.2621404149511164No Hit
TTAAGGCAAGCTCACGTGTGCAAAAATCTCTGAAGCAAGCAGAGATATAC22140.26202206713398274No Hit
TTGAGTGCCTATATCATGGAGGAACCCAGCACAGGAAGGCTCATCTTTGG21490.2543294590202931No Hit
TTGAGTGCCTATATCGTGAAGGAACCCAGCACAGGAAGGCTCATCTTTGG20620.24403319892966233No Hit
GCCGCCTTACACTGACTTGAGTGCCTATACCTGCAACTGTTTGGAAATCC19980.23645893863310638No Hit
TTGAGTGCCTATAGGGGGAAAAATACATGCATATTCTGCAGTCTTTGATG19710.23326354757049686No Hit
TTGAGTCCCTATATCATGAAGGAACCCAGCACAGGAAGGCTCATCTTTGG18020.21326276647490378No Hit
TTGAGTGCCTATATTCTGCAGTCTTTGATGAGTCACATTCAGTTGGTGAT17820.21089581013223002No Hit
GCCGCCTTACACTGACTTGAGTTGATAACCAAAGATGAGCCTTCCTGTGC17530.20746372343535308No Hit
TGAGTGCCTATATCATGAAGGAACCCAGCACAGGAAGGCTCATCTTTGGT16620.19669407207618758No Hit
TCAAGGCCCTGGGCAGAGCACAAGATGTGCAATGTGACGATCCCACTTGT16200.19172346375657276No Hit
TTGAGCCCTATATCATGAAGGAACCCAGCACAGGAAGGCTCATCTTTGGT15910.1882913770596958No Hit
TTGAGTGCCTATAGTGGACTAAGGATCAGCAGAGGCTGCAGACTGCGAGA15800.18698955107122528No Hit
TTGAGTCTAAATTTTGGATTTCCAAACAGTTGCAGGTAAGACCCACATGT15490.18332076874008096No Hit
TCCAGTTCTGTATCATATGGCGTGGCCTCAGCTGCAGTATTTTCTCTGCC14530.17195937829524705No Hit
TGAGATTCGTAAGCCAAAGGCTTTTGTTAGATTGTGAGGCAAGGTAGAGG14290.16911903068403855No Hit
TTGAGTGCCTATAACCCTTGGAAGAATCAATGTAAGCTGGTTAGCTACAA13690.16201816165601735No Hit
CAAGGAACTGCTCAGAGGAGCCGTTTACAGCACAGAAGCAAGAGAAGAGA13410.1587044227762741No Hit
TTGAGTGCCTATAGTAAAAACAGTGGCACTATTGCTAGGCAGTTCATGCC13260.1569292055192688No Hit
TTGAGTACAAGCTTCCTTACCACTCCTGTTTTATTTAAAACTCCGCTCCT11920.14107059802335475No Hit
TTGAGTGCCTATATTGCTAGGCAGTTCATGCCATTTGAGAATTTGAACCC11490.13598164188660622No Hit
TTTAGGCAATCTTTTGAGAAATGTCATTCTGCCTAGCAGAGTCTAAATTT11360.1344431202638683No Hit
TTGAGTCTTCCTGTGCTGGGTTCCTTCATGATATAGGGCTCACTTTGGGT11330.13408807681246723No Hit
TTGAGTGCCTATATTCATTAAGGCAAGCTCACGTGTGCAAAAATCTCTGA11300.13373303336106618No Hit
TCAAGGGGGGGGAAGAGAAAGGCTGGCAAGATACAAGGTGATGCCCAAGA10820.1280523381386492No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT10200.1207147734763606TruSeq Adapter, Index 7 (97% over 38bp)
TTGAGGTCACACAGCTGCTTCCAGGGCCTCAGATTTTAAACCCAGCATCC10150.12012303439069218No Hit
TTGAGTTCCAAACAGTTGCAGGTAAGACCCACATGTCCTTTTCATTGCAA9880.11692764332808263No Hit
TTGAGTGCCTATACTTTGCAATGAAAAGGACATGTGGGTCTTACCTGCAA9880.11692764332808263No Hit
TTGAGTGCCTATAGAAGACAAGTTTTGTATTTCCATCTAAATAGATACAT9510.11254877409413622No Hit
TGAGTCCCTTTTCCCTTTGCTGATTCTAATCTGTATCTTTTCCCTGTAAT9200.10887999176299192No Hit
TTGAGTGCCAAAGGCTTTTGTTAGATTGTGAGGCAAGGTAGAGGGCAGGG9170.10852494831159087No Hit
GCCGCCTTACACTGACTTGAGTGCCTATATCATGAAGGAACCCAGCACAG8930.1056846007003824No Hit
GCCGCCTTACACTGACTTGAGTGCCTATAAGAAGATAAGTTTTGTATTTC8830.10450112252904552No Hit
TTGAGTTAAGATCTGAATGGTGGATCAGAAGAAGGCAGCTCAGAGTGGAT8510.10071399238076753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGTT1200.0144.008471
TCGAGGT202.2044242E-6144.008471
CGTATAT253.8675353E-8143.999958
CCGATAG100.0072389087143.999948
GTTAACC202.2050735E-6143.999945
AGTTGCC151.2643177E-4143.999944
CGAGGTC202.2050735E-6143.999942
AGTTCGA100.0072389087143.999944
GAGTTCG100.0072389087143.999943
AGTTAAC202.2050735E-6143.999944
TGCGTGC100.0072389087143.999942
TCCGGGA100.0072389087143.999947
CGCCTTA39400.0142.903493
CCGCCTT39600.0142.72722
CTTACAC39800.0141.467296
GCCGCCT39800.0141.294741
TTACACT39850.0141.289787
TAGAGTG7500.0141.12831
AGAGTGC7250.0141.020632
TCAAGGG15250.0140.703351
Produced by FastQC (version 0.11.2)