FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_ny238_plasmid_mix_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_ny238_plasmid_mix_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673657
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAAC628219.325368844976003No Hit
TCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGC463526.880652913871598No Hit
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACT416096.176585413645223No Hit
TCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG392695.82922763364739No Hit
TCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGG363765.399780600513317No Hit
TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTT349305.185131305694144No Hit
TCAAGTCAAGGCTTTTCTATGGAATAAGGAATGGACAGCAGGGGGCTGTT308034.572504998834718No Hit
TCAAGCATCAGGAAGAATCACAGTCTCTACCAAAAGAAGCCAACAAACTG118111.7532661280147017No Hit
TTGAGGTTAGATTTTTTTTATATTTTGTTTTGTGTTATTTTTTTCTTTAA69331.0291587558653736No Hit
TTGAGCATATAGGCTGATTTGGTCATTGTCCGTACCCGGGTGGTGAACTC60910.9041693324644442No Hit
TTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA57760.8574096313108897No Hit
TTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAA51430.7634448985166042No Hit
TTGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTG48390.718318075816031No Hit
TGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACC43730.6491434068079156No Hit
TTGAGTGCTTTTGAGATCTGCTGCTTGTCCTATTCCCTCAGAATTTTGAT39490.5862033646202741No Hit
TGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG37340.5542880130392767No Hit
TTGAGTGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA28070.41668089250167367No Hit
TCGAGAAATATGTTGAGGACACTAAAATAGATCTCTGGTCATACAACGCG25960.3853593149035785No Hit
CAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCA24690.3665069909464312No Hit
TTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCC24350.3614599120917618No Hit
TTGAGTAGCTAAGACCCCGTAATTGATTACTATTAATAACTAGTCAATAA24270.36027236412595726No Hit
TTGAGTAGCTAAGGACCCCGTAATTGATTACTATTAATAACTAGTCAATA24230.359678590143055No Hit
TCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAGCGGTTCCTCTTGGG24080.35745193770717143No Hit
CAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGT21520.31945040280142567No Hit
CAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGA20680.3069811491604778No Hit
TCAAGGATGTGATGGAATCAATGGATAAAGAGGAAATGGAGATAACAACA19470.2890194861776839No Hit
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTT19260.28590217276744695No Hit
TTGAGTAGCTAAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAG19170.2845661813059168No Hit
CAAGTCAAGGCTTTTCTATGGAATAAGGAATGGACAGCAGGGGGCTGTTT15700.23305628828914418No Hit
GCCGCCTTACACTGACTTGAGTCCAACCCGGTAAGACACGACTTATCGCC15360.22800920943447484No Hit
TTGAGTAGCTAAGCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAG14060.20871155499015076No Hit
TCGAGCATGCCCGGGTACCATGCAGGTCAATTCTAGACGGGCCGGCCCCT13930.20678178954571835No Hit
TTGAGCATCTGACTTCTGGCTAATAAAGGAAATTTATTTTCATTGCAATA13340.19802362329790976No Hit
TTGAGTAGCTAAGCACAGGGAATCTAATTGCTCCTCGGGGTTACTTCAAA12310.1827339432381761No Hit
TAGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAAC11880.17635087292197663No Hit
TTGAGTAGCTAAGAAAGGAAATTTATTTTCATTGCAATAGTGTGTTGGAA11680.17338200300746523No Hit
TTGAGTAGCTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATAC11330.1681864806570703No Hit
TTGAGTATCTGACTTCTGGCTAATAAAGGAAATTTATTTTCATTGCAATA11260.1671473761869913No Hit
GCCGCCTTACACTGACTTGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGC11040.16388161928102876No Hit
TTGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCG10810.16046741887934066No Hit
GCCGCCTTACACTGACTTGAGTGGCGAAACCCGACAGGACTATAAAGATA9810.14562306930678373No Hit
TTGAGTAGCTAAGAATGGTTACAAATAAAGCAATAGCATCACAAATTTCA9780.14517773881960702No Hit
GCCGCCTTACACTGACTTGAGTTAGCTCACTCATTAGGCACCCCAGGCTT9780.14517773881960702No Hit
CAAGCATCAGGAAGAATCACAGTCTCTACCAAAAGAAGCCAACAAACTGT9650.14324797337517461No Hit
TGAGTGCTTTTGAGATCTGCTGCTTGTCCTATTCCCTCAGAATTTTGATG9400.13953688598203537No Hit
TTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA9150.13582579858889612No Hit
TTGAGTAGCTAAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTT9120.13538046810171941No Hit
TGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAA8910.13226315469148245No Hit
TAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTG8750.12988805875987333No Hit
TGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGC8680.12884895428979437No Hit
TTGAGTAGCTAAGCCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCC8660.12855206729834323No Hit
TGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAG7390.10969974334119588No Hit
TTGAGGAAATCTATTAGCCTTCCTGATTCATTAGCTGTTAGTCCATTCGA7340.10895752586256804No Hit
TTGAGTGACTCCAGTCCAATTGAAGCTTTCATTGTTAAACTCCAGTGTGC7250.10762153440103792No Hit
GCCGCCTTACACTGACTTGAGTACTCACCAGTCACAGAAAAGCATCTTAC6830.10138690758056398No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAGTC100.0072355415144.02021
TCGCTAA100.0072355415144.02026
CTTAGCG100.0072355415144.02029
TAGAGTG650.0144.020191
GTCGCTA100.007238754143.998835
AGTATAG100.007238754143.998834
CAAGGTT100.007238754143.998832
CAAGGGG202.2048316E-6143.998832
GAAGTTC100.007238754143.998835
GTATAGC100.007238754143.998835
TCAAGCA14450.0142.525181
TCAAGAC42700.0141.490551
CTTACAC34850.0141.334016
TCAAGGA57450.0141.262631
TACACTG35050.0140.938458
GCCTTAC34950.0140.90874
TCAAGTC73350.0140.093261
ACACTGA35900.0137.200299
TAGAGTC950.0136.440191
GATAGTT44550.0135.453348
Produced by FastQC (version 0.11.2)