FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_lp_negative_control_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_lp_negative_control_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4182
Sequences flagged as poor quality0
Sequence length150
%GC71

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT352284.2180774748924TruSeq Adapter, Index 2 (97% over 37bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1794.28024868483979TruSeq Adapter, Index 2 (97% over 35bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT431.0282161645145864TruSeq Adapter, Index 2 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG310.741272118603539TruSeq Adapter, Index 2 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT240.5738880918220948TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATGTCGTAT220.5260640841702534TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATATCGTAT150.3586800573888092TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTAGCTCGTAT120.2869440459110474TruSeq Adapter, Index 2 (97% over 37bp)
ACTCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT70.1673840267814443TruSeq Adapter, Index 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGA151.1762792E-4144.000021
AATCGGA151.1762792E-4144.000021
GATCGGA3550.0144.01
TTGAGTT100.006983203144.01
TGAGTTA100.006983203144.02
GAAGAGC3950.0142.177226
TCGGAAG3950.0142.177223
GAGCACA3950.0142.177229
CGGAAGA3950.0142.177224
AGAGCAC3950.0142.177228
ATCGGAA3950.0142.177222
GGAAGAG3950.0142.177225
AAGAGCA4000.0140.47
TTCTGCT3800.028.80000155-59
TCTGCTT3800.028.80000155-59
AAAAGGG3300.028.80000170-74
AAAGGGG3300.028.80000170-74
GCTTGAA3800.028.80000160-64
CTGCTTG3800.028.80000155-59
AAAAAGG3300.028.80000170-74
Produced by FastQC (version 0.11.2)