FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_lp_negative_control_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_lp_negative_control_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAACAACAGTAGATAAATATAACATAATAAGAAAATTCAAAACATCAAG18.333333333333332No Hit
TAATTGTACTACTCATTGTAGTAACATCCAATGCATCACGAATCTGCACT18.333333333333332No Hit
CGTGACCTTGGGCTTCCTGTGTGTTCCAACATGATGTGTAGGCTTAAGAC18.333333333333332No Hit
GTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGG18.333333333333332No Hit
AAACAATAAATGGCTGTTGCTGCTGAGTGCCAAGTCGTTTTCCCACAATC18.333333333333332No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG18.333333333333332No Hit
CCCTTAATTGAGCCTGGGAAACCCAGGCTTCACTAGCTTTAAGCAGCTGT18.333333333333332No Hit
GACCATGATTACGCCAAGCTTGGGCGGTCGAGGGATCTCCATAAGAGAAG18.333333333333332No Hit
ACACGAGCATTTTTCAGAAACAATTAAGCTCAGAAAGAACAATTGATCAA18.333333333333332No Hit
CTTTAAGACTTTCATTATCTTTGCCTGTTATTTTAGCTGCCTCTTAATTG18.333333333333332No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGACGT18.333333333333332No Hit
TTACCCATCCACTTGGATTTGTCTTCATCTCTCATTTCCAAAGCTTCGTG18.333333333333332No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)