FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_cal-09_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_cal-09_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807800
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGGGTCATGTCAGAAAGGTAGCGCGAAGTAGGTACAGATGCAATTGT14139717.50396137657836No Hit
TCGAGGAAATGTCACGAGACTGGTTCATGCTCATGCCTAGGCAAAAGATA509036.301435999009655No Hit
TCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCTTGGG457435.662664025748948No Hit
TTGAGTTCATCTGGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGAC235702.9178014359990097No Hit
TTGAGTCAGTCGCTTGGTCAGCAAGTGCTTGTCATGATGGCATCAATTGG191622.3721218123297847No Hit
TCAAGAATGGTTCAATTTTGGCGTTCACTTCTTTTGACATTTGGGAAAGC117971.4603862342163902No Hit
TCAAGGCACCAAACGATCATATGAACAAATGGAGACTGGTGGGGAGCGCC111771.3836345630106461No Hit
TTGAGTTCATCTGGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGAC101091.2514236197078485No Hit
TTGAGTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCT75820.9385986630354047No Hit
TCAAGATCTGTGTTCTTTCCTGCAAAGACACTTTCCAGTCTCTGCGCGAT75440.9338945283486012No Hit
CGAGGGTCATGTCAGAAAGGTAGCGCGAAGTAGGTACAGATGCAATTGTC71200.8814062886853182No Hit
TCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTGTCATGATGGCAT70780.8762069819262194No Hit
TCAAGAGTTGGTGCATGGGGTTCAGTCGCTGGTTTGCCCTATTGACAAAG61560.7620698192621936No Hit
TTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTG61490.7612032681356772No Hit
TTGAGTTAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAAGC53920.6674919534538252No Hit
TCGAGGGTCATGTCAGAAAGGTAGCGCGAAGTAGATACAGATGCAATTGT48860.6048526863084922No Hit
TTGAGTTCATCTGTCCCTTTTCTATTCTGAAGATCTTGTATGAGGCCTGT43200.5347858380787324No Hit
TTGAGGATGAACAGATGTACCAGAAGTGCTGCAATCTATTCGAGAAATTT42350.524263431542461No Hit
TTGAGTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAA40150.4970289675662293No Hit
TGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCGAATC38910.48167863332508043No Hit
TTGAGTTAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAA36220.4483783114632335No Hit
TTGAGTCAAGAAGAAGGTTCTGCATTCCAAGGGGGAGCATGATATGAATG35670.44156969546917557No Hit
TTGAGTTAAAGAAGCTGTGGGATCAAACCCAATCAAAGGTAGGGCTATTA34090.42201039861351813No Hit
CGAGGAAATGTCACGAGACTGGTTCATGCTCATGCCTAGGCAAAAGATAA33570.41557316167368163No Hit
TCAAGGGGCCTTGCTAAATGACAAACATTCCAATGGAACCATTAAAGACA28850.35714285714285715No Hit
TTGAGTTCATCTGAAGATCTTGTATGAGGCCTGTCCATTACTTGGTCCAT28020.34686803664273336No Hit
TGAGTCAGTCGCTTGGTCAGCAAGTGCTTGTCATGATGGCATCAATTGGC25000.3094825451844516No Hit
CAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCTTGGGA24120.2985887595939589No Hit
TTGAGTTCATCTGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTT23940.29636048526863085No Hit
TTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTC20930.25909878682842286No Hit
GCCGCCTTACACTGACTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTGTCA20530.25414706610547166No Hit
TTGAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAA17910.22171329537014114No Hit
TGAGTTCATCTGGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACT17840.22084674424362466No Hit
TTGAGTGCATCTGTCGGAAGAATGATTGGTGGAATCGGGAGATTCTACAT17220.2131715771230503No Hit
TTGAGTTCATCTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACC15950.1974498638276801No Hit
TTGAGTTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATA14070.17417677642980936No Hit
TTGAGTTCATCTGGGGGGTGCGAAAGCAGGGTGACAAAAACATAATGGAC13510.16724436741767765No Hit
TTGAGTCAATCTGGACCCCTCTTGTGGAAAGCTTTCCTCTTGGAATCACT11990.14842782867046297No Hit
TTGAGTTCATCTGATTTGACTATCTTTCCCTTTTCTATTCTGAAGATCTT11890.1471898984897252No Hit
TTGAGTTCATCTGGGGGGGGCGAAAGCAGGGTGACAAAAACATAATGGAC11710.14496162416439712No Hit
TTGAGTTCATCTGAGGGGGGAGCAATAGTTGGAGAAATTTCACCATTACC11410.1412478336221837No Hit
TTGAGTTCATCTGGGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACC11060.1369150779896014No Hit
TCAAGTTGTGGAGACGCATATAGACTGTTGAATACAGATTTTGCCAGTAA10200.12626887843525625No Hit
TCAAGGTCGCCAGACTTATGATTGGACATTAAACAGAAATCAACCGGCAG10040.12428819014607576No Hit
TTGAGTTCATCTGGGTGACAAAAACATAATGGACTCCAACACCATGTCAA9560.11834612527853428No Hit
TGAGTTAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAAGCA9350.1157464718989849No Hit
TTGAGTGCATCTGCACTCCCATTCGCTTCTGGTAGGCCTGCAAATTTTCA9260.11463233473632088No Hit
TTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTTTCCAGAACTGGGGAAT9130.11302302550136173No Hit
TTGAGTGCATCCGCAGGCCAGATCAGTGTGCAGCCTACATTCTCAGTGCA8710.10782371874226294No Hit
TTGAGTTCATCTGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACT8530.10559544441693489No Hit
TGAGTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTT8440.10448130725427085No Hit
TTGAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGAA8380.10373854914582817No Hit
TCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAATTTGAG8200.10151027482050011No Hit
GCCGCCTTACACTGACTTGAGTTAAGCTGAAACGAGAAAGCTCTTATCTC8190.10138648180242635No Hit
TTGAGTCCAAATGTCCAGGAAACCATCATCAACTTTTTTATTTAAATTCT8120.10051993067590988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGTT100.0072335866144.034991
TCGACGG100.0072335866144.034991
CGTCTGG253.86608E-8144.008248
GTCACGT100.0072376044144.008227
CAAGTAT151.2643191E-4143.999312
GAGCGTC100.007238944143.999313
CGAGCGT100.007238944143.999312
TCGAGGG163650.0143.110821
CCGCCTT46600.0142.917792
GCCGCCT46800.0142.650041
CGCCTTA46550.0142.607273
TAGAGTT6200.0141.711841
GCCTTAC47650.0139.16414
TCGAGGA59550.0138.955661
GCCAGAC1300.0138.469459
CGCCAGA1300.0138.469458
CCTTACA47900.0138.137135
TAGAGTA1150.0137.772581
GTGAGTT1900.0136.454181
GAGGGTC174250.0134.867653
Produced by FastQC (version 0.11.2)