FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_463_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_463_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820088
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCC17323021.12334286076616No Hit
TTGAGTTATCCGGCCCCAAGTGGCGTTGTCAAGTCTCATTTTTCTGAGAA692598.445313185902No Hit
TTGAGTTAACCAGTAGACCAATATAACATAATAAGAAAATTCAATACATC454055.536600950141936No Hit
TCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAGC401524.896059935031363No Hit
TTGAGTTAACCAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATG120151.4650866736252695No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTATAGAATTCGCCCAGTTTCAC102411.248768424851967No Hit
TTGAGTTAACCAGGGGAGCATGCAAAGGCCATAGGAAATTGCCCAATATG82681.0081844875184127No Hit
TCAAGGGGAGGTCAATGTGACCGGTGTAATACCACTGACAACAACACCCA82631.0075747968510695No Hit
TCAAGAATCATGTTCCTCTGATCAGTGTTTAGTATAGATCTGTTTCTCTT76050.9273395050287286No Hit
TGAGTTATCCGGCCCCAAGTGGCGTTGTCAAGTCTCATTTTTCTGAGAAA75090.9156334442157428No Hit
TTGAGTTAACCAGGGGACATGCAAAGGCCATAGGAAATTGCCCAATATGG59130.7210201831998517No Hit
TCAAGCCGTCCAAATCTTTGTCTTTGTCTGATCTTTAGTCCCAATGCAGC59110.7207763069329145No Hit
TTGAGTTAACCAGGAGACCAATATAACATAATAAGAAAATTCAATACATC58070.7080947410521797No Hit
TGAGTTAACCAGTAGACCAATATAACATAATAAGAAAATTCAATACATCA57970.7068753597174937No Hit
TTGAGTTAACCAGGGGGACATGCAAAGGCCATAGGAAATTGCCCAATATG56460.6884627015637347No Hit
TTGAGTTAACCAGGGGACCATGCAAAGGCCATAGGAAATTGCCCAATATG54360.6628556935353279No Hit
TTGAGTTAACCAGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGG46960.5726214747685614No Hit
TTGAGTTAACCAGATTGCCTCCACGAAAAATACGGTGGATTAAACAAAAG44700.545063456604657No Hit
TAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCCG42630.5198222629766561No Hit
TTGAGTTAACCAGTTTTGAAGCAAACAACAGTAGACCAATATAACATAAT38130.46495010291578465No Hit
TTAAGGAATCTGCATTCACTAACACCTGAAGCTGAGCCATCAGTTATCAT34430.41983299353240144No Hit
TTGAGTTTCCTTTGGTTTCTCCGAATCTATGAGGGCTAATTAGGAGTGCT32500.39629893377296094No Hit
TTGAGTTAACCAGGGGATCATGCAAAGGCCATAGGAAATTGCCCAATATG30030.36618021480621593No Hit
TTGAGTTAACCAGGGGAATATGCAAAGGCCATAGGAAATTGCCCAATATG27030.32959877476563487No Hit
TTGAGTGCTCTTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGG25950.3164294563510257No Hit
TTGAGTAGCAGTTTTGACGACATGTGGTGAGTTTGACGATGTTATCCCAG25910.3159417038171513No Hit
TAGAGTTATCCGGCCCCAAGTGGCGTTGTCAAGTCTCATTTTTCTGAGAA24360.2970412931295178No Hit
TTAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAACTCCAGATCCATCCT20410.24887573040941946No Hit
TTGAGTTAACCAGGGGTACATGCAAAGGCCATAGGAAATTGCCCAATATG20320.24777828720820202No Hit
TTAAGATTCGAGAGGGCCGAATAATAAAAGAAATATTTCCAACAGGAAGA20100.24509564827189276No Hit
CAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAGCT19300.2353405975944045No Hit
TCAAGACACTAGGATGTTCCAAGGGGTGAGAGGAGAAATAAATTTTCTTA18910.23058501038912896No Hit
TTGAGTTAACCAGTTGACCAATATAACATAATAAGAAAATTCAATACATC17150.20912389889865476No Hit
TAAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCC16310.1988810956872921No Hit
TTGAGTTAACCAGCAAAGGCCATAGGAAATTGCCCAATATGGGTGAAAAC16290.19863721942035487No Hit
GCCGCCTTACACTGACTTGAGTTATCCGGCCCCAAGTGGCGTTGTCAAGT16290.19863721942035487No Hit
TTAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAATTCCAGATCCATCCT15790.1925403127469247No Hit
TTGAGTACCAGCAGCTATTCTGTCGAGACAGGTCTGGTTGCACTTGTGTT15630.19058930261142706No Hit
TAGAGTTAACCAGTAGACCAATATAACATAATAAGAAAATTCAATACATC15380.18754084927471198No Hit
TTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCT14490.17668835539600627No Hit
TTGAGCTAAAAAGAATATCAGGAAGAGGATTCAAAAATGATGAAGAAATA14140.17242052072460518No Hit
TTGAGTTAACCAGGGGGACAACCAGACCAATCATTAGACCAGCAACCCTT12960.15803182097530996No Hit
TTGAGTCCCCATAGAGCTTTGCCATTTGGGTCTTGTTGTCAGAGTGGAAC12910.15742213030796695No Hit
TCAAGAAGGGAAACTAGTAAAAGGATCCTTTGGAAAAGCACTAAGAGTAA12420.15144716176800538No Hit
TTGAGTTAACCAGTAGCCCAATATAACATAATAAGAAAATTCAATACATC12020.14656963642926124No Hit
TCAAGATGATGAATGACTCAATGGCTAAGAAAACCAGTGGAAATGCTTTC11740.14315536869214035No Hit
TTGAGTTAACCAGTGGACCAATATAACATAATAAGAAAATTCAATACATC11270.137424276419116No Hit
TTGAGAAAATACCATGCATAAGATTATTAAATCTCTCATATCCTCCTTTT11220.136814585751773No Hit
TTGAGTAGATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA10890.13279062734730906No Hit
TTGAGTTCATCCATGGCACCGCTTAGTCTTTGAAGATTCTTTACTTCCAG10320.12584015373959867No Hit
TTGAGTTAACCAGAAGACGGAGGACTACCACAAAGTGGTAGAATTGTTGT10290.12547433933919286No Hit
TTGAGTTAACCAGGGGCACATGCAAAGGCCATAGGAAATTGCCCAATATG10080.12291363853635219No Hit
TGAGTCCAGCTTTGACCATCATCTGGTTATAGAATTCGCCCAGTTTCACC9700.11827998946454528No Hit
TTGAGTTCCAATACTGAAAGTTTTGGGGCTATTTACTCTGTCTCCCTTGA9390.11449990732701855No Hit
GCCGCCTTACACTGACTTGAGTGCTCTTAAGGTCAGCTGCCACCGCTACT8850.10791524811971398No Hit
TTAAGGTCAGCTGCCCCCGCTACTCCATGTGCCCCATGGGATGTGTATCC8810.10742749558583957No Hit
TTGAGTAGTAAAGCAGTATAGTATGATTATCCAATCCATCGTCATTTAAA8740.10657392865155933No Hit
TTGAGTTAACCAGAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATA8700.10608617611768494No Hit
TTGAGTTAACCAGGGAAACATGCAAAGGCCATAGGAAATTGCCCAATATG8570.10450098038259309No Hit
TGAGTTAACCAGGAGACCAATATAACATAATAAGAAAATTCAATACATCA8250.10059896011159779No Hit
GCCGCCTTACACTGACTTGAGTTAACCAGTAGACCAATATAACATAATAA8250.10059896011159779No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGCG151.2634197E-4144.025241
ACCGGGG450.0144.016469
TTAGCTG151.2637267E-4144.016467
GTCCGGC151.2637267E-4144.016468
CGCCGTA100.0072390265143.99893
GCCGCCT35950.0143.824941
CGCCTTA36550.0142.029023
TAGAGTT10350.0141.242161
CTTACAC36600.0140.868566
CCGCCTT36950.0140.500062
TTACACT36600.0140.475087
TTAAGGC3950.0140.379031
TTAAGGT208600.0140.02071
AGAGTTA10250.0139.090352
CCTTACA37100.0138.953115
CTAGGAT2650.0138.581889
TCAAGAA12600.0138.309951
TCAAGAG44950.0137.29661
TAAGGTC213900.0136.73662
AAGGTCA216450.0136.647613
Produced by FastQC (version 0.11.2)