FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_463_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_463_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962256
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC109210.5565532733751355No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT86180.43918836278243006No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT32120.16368914147797228No Hit
CAACAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG31100.15849104296279384No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA30000.15288525044642493No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA27160.13841211340416337No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG26750.13632268164806222No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAATC26360.1343351733922587No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC26250.13377459414062182No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC25770.131328430133479No Hit
AGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAAT25090.12786303112336006No Hit
CAACAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAA22840.11639663733987818No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT21710.11063795957306284No Hit
CACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG21550.10982257157068191No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA21230.10819179556592004No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC20980.10691775181219984No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGT20730.10564370805847964No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC20640.10518505230714034No Hit
ACAACAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAA20610.10503216705669394No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT19990.1018725385474678No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA39500.0129.967534
CGGTGCA1400.0113.148322
TAACAAG46050.0111.165835
AGCACGC5150.0106.281941
GCACGCA5900.096.411432
TAGTAAC52700.095.640292
GAAACAC19850.093.5844
CACGCAC6300.092.573543
AAACACG20100.092.059465
AACACGT4200.090.85696
TAGAAAC23300.090.854172
ACAAGAG56450.090.557787
CGCACTT6900.087.651955
GAAGCGG5300.085.608671
AGTAACA61200.084.590173
ACGTGCA9000.083.2040252
ACGCACT7600.079.580774
CAAGAGC57500.078.2606668
CGGTGCG3300.067.639632
CTCGCTC755.131369E-967.201543
Produced by FastQC (version 0.11.2)