FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_460_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_460_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1471860
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCC53274436.195290312937374No Hit
TTGAGTTATCCGCAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATA545883.70877664995312No Hit
TTGAGTTATCCGCACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAA400652.722065957360075No Hit
TCAAGGGGAGGTCAATGTGACCGGTGTAATACCACTGACAACAACACCCA370252.515524574348104No Hit
TTGAGTTATCCGCCATAGGAAATTGCCCAATATGGGTGAAAACACCCTTG247711.6829725653255065No Hit
TTGAGTGGCTCTGGGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCA228221.5505550799668446No Hit
TAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCCG208491.416507004742299No Hit
TTGAGTTATCCGCAAAGGCCATAGGAAATTGCCCAATATGGGTGAAAACA183661.2478088948677184No Hit
TTGAGTGCTCTTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGG126710.8608835079423314No Hit
TTGAGTAGCAGTTTTGACGACATGTGGTGAGTTTGACGATGTTATCCCAG111290.7561181090592856No Hit
TCAAGAACCCTGCAGAAATGGACAGCAGGCATCTCCAAAGTGACCTGTGT99830.6782574429633254No Hit
TTGAGTATCTGTGGGCATCAGGAGGAGGAGTTCATGAGAGGACAGGCTGG89350.6070550188197247No Hit
TCAAGCTCACTCTCCGCCACCCCTAAAGACCTCTTCCTCCCTCCTCTGAA72100.48985637220931333No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTATAGAATTCGCCCAGTTTCAC63850.4338048455695515No Hit
TTGAGTCCCCATAGAGCTTTGCCATTTGGGTCTTGTTGTCAGAGTGGAAC62110.4219830690418926No Hit
TTGAGTTATCCGCATGCAAAGGCCATAGGAAATTGCCCAATATGGGTGAA60890.413694237223649No Hit
TCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAGC55030.37388066799831504No Hit
TCAAGATTCTCAGGTGGTCAGCTGGTTCTTCTACTGGTGTTTTCTATCCC50010.33977416330357507No Hit
TTGAGTTATCCGCAATGGCCATAGGAAATTGCCCAATATGGGTGAAAACA49090.33352356881768647No Hit
TAAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCC48280.328020328020328No Hit
GCCGCCTTACACTGACTTGAGTGCTCTTAAGGTCAGCTGCCACCGCTACT47830.3249629720217956No Hit
TGAGTTATCCGCAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATAT43010.2922152922152922No Hit
TTGAGTTTCCTTTGGTTTCTCCGAATCTATGAGGGCTAATTAGGAGTGCT40630.276045276045276No Hit
TTGAGTTATCCGCCTCCACGAAAAATACGGTGGATTAAACAAAAGCAAGC35050.23813406166347342No Hit
TGAGTTATCCGCACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAAT32550.22114875056051525No Hit
TGAGTGGCTCTGGGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCAA30570.20769638416697242No Hit
TTGAGTTATCCGCAGAGGCCATAGGAAATTGCCCAATATGGGTGAAAACA30280.20572608807902926No Hit
TCGAGATTGGGAATAGGTAATAAAGAAAATGAATCCTGAGGGCTGATAAA29510.20049461225931814No Hit
TTGAGTTATCCGCCAGCTGGACGAGATGAGGACTCAGAGTCAAGATCAAG28120.1910507792860734No Hit
TTGAGTTATCCGCTGGACGAGATGAGGACTCAGAGTCAAGATCAAGTTGC26820.18221841751253517No Hit
TTGAGTTATCCGCGGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATA26370.17916106151400268No Hit
TTGAGTTATCCGCATAGGCCATAGGAAATTGCCCAATATGGGTGAAAACA24130.16394222276575218No Hit
TTGAGTTATCCGCTGGCCAATGGAACCAAATATAGACCTCCTGCAAAACT23660.16074898427839604No Hit
TTGAGTCCAATAGCTGCCCCAGCTTACCACATTAATTAGTCTTCAGCCGA23400.1589825119236884No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT22850.1552457434810376TruSeq Adapter, Index 13 (97% over 38bp)
CAAGGGGAGGTCAATGTGACCGGTGTAATACCACTGACAACAACACCCAC22490.15279985868221163No Hit
TTGAGTTGTCCACCTGGACCCAATGTCATCACGAAAGTCCTTCTAAGAAG22160.15055779761662114No Hit
GCCGCCTTACACTGACTTGACAACGCCACTTGGGGCCGGATAACTTTTGG21250.1443751443751444No Hit
TTGAGTAGCTGGGATTACAGGCACCTGCCACCATGCCGAACTAATTTTTT21230.1442392618863207No Hit
TTGAGTTATCCGCAACGGCCATAGGAAATTGCCCAATATGGGTGAAAACA20960.14240484828720124No Hit
TTGAGTTGATAACCTGATATGTTCGTATCCTCGGAGAAGGTTAGGCAGCT20890.14192925957631838No Hit
TTAAGGTCAGCTGCCCCCGCTACTCCATGTGCCCCATGGGATGTGTATCC19770.13431984020219315No Hit
TTGAGTTATCCGCAAGGGCCATAGGAAATTGCCCAATATGGGTGAAAACA19650.13350454526925115No Hit
TGAGTTATCCGCCATAGGAAATTGCCCAATATGGGTGAAAACACCCTTGA19090.1296998355821885No Hit
TTGAGTCCTCATCTCGTCCAGCTGGCAGGGAAATCCAACTTATTACTCCT17620.11971247265364912No Hit
TTGAGTTATCCGCAAGCCTTACTACACAGGGGAACATGCAAAGGCCATAG17360.11794600029894148No Hit
TCAAGCCGTCCAAATCTTTGTCTTTGTCTGATCTTTAGTCCCAATGCAGC16590.11271452447923036No Hit
TTGAGTTATCCGCACAGGCCATAGGAAATTGCCCAATATGGGTGAAAACA16020.10884187354775589No Hit
TTAAGCATGTTTTGAGAATGGCAGCCCAGTCATCCTTACCTGGCACCTGC15940.10829834359246124No Hit
TTGAGTTATCCGCTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCC15850.10768687239275473No Hit
TTGAGTAAAACACACTGAGGAATGCACATGCGGATTTGCCAGCAATAAAA15700.10666775372657725No Hit
TTGAGTTATCCGCACACCCCAGATGCCCTGGCACTATGGACGGCACCAGG15400.10462951639422227No Hit
TTGAGTTATCCGCACAACGTTATCAATGCAGAAAATGCACCAGGAGGACC15060.10231951408421996No Hit
TGAGTTATCCGCAAAGGCCATAGGAAATTGCCCAATATGGGTGAAAACAC14890.10116451292921881No Hit
TTGAGTAGTAAAGCAGTATAGTATGATTATCCAATCCATCGTCATTTAAA14860.10096068919598332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGCG306.82121E-10144.024051
TTACGCT202.2052773E-6144.004496
GTTCGCG100.007239503143.999595
TTAAGGT603750.0138.52551
TTGAGTT453900.0138.502981
CCTTACA31550.0136.925135
TCAAGGC1850.0136.238971
CTTACAC31750.0136.067236
TTGAGTA46900.0135.272061
TAAGGTC626500.0133.644942
CGCCTTA32500.0133.365783
TTACACT32600.0132.519457
AGTTACG600.0131.999624
TTGTCCG3400.0131.29826
GGTCAGC647300.0131.263665
AGCTGCC646850.0131.248129
AGGTCAA45350.0130.985349
TACACTG33000.0130.913188
CGAGATT3250.0130.707322
GTCAGCT650550.0130.534846
Produced by FastQC (version 0.11.2)