FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_460_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_460_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4715998
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC109190.23153105662894682No Hit
GTAGAAACACGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC63010.13360904733208115No Hit
AAATTACACAATCTCAGTTATTTCTTTATAGCAACGCAAAAAATGGATGA59940.12709929054253202No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGT20400.088.2375956
AAACACG57700.087.347855
ACACGTG21700.082.951477
AACACGA41200.079.69146
GTAACAA24050.079.334274
ACGTGCC9500.077.307289
ACACGAG45350.072.240037
TAGAAAC95700.067.035522
GAAACAC77100.066.0229644
AGATCCG554.233676E-665.456259
GAAGCGG15100.059.139911
CACGTGC30750.059.0064168
CACGAGC55900.058.477378
ACGAGCT9100.056.9685179
TAGTAAC35300.056.0915152
AAGCGGA14550.050.969862
GTAGAAA131900.049.5220261
AGTAACA41500.049.2720033
TAACAAG40300.046.9873245
ACGAGCC27100.046.229999
Produced by FastQC (version 0.11.2)