FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_428_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_428_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566745
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTGTTTTGGAGGGAAAAGCAGTTTGTAAATGTTTGTTGAATGACAG278304.910497666499043No Hit
TTGAGTCTCTGCTTGTGATTCTTTGGCTTTGTATCTAGGAGTGGAAGCGC270094.765635338644364No Hit
TCAAGGATTGGAGGTGGCAGCCCCACCTGGCAGACACCATCGTATATGCC260354.593776742626755No Hit
TTGAGTACTAACGGGGGGAAAATCATGGGAAAAATACTTAAAATGTCTAA108921.9218519792852167No Hit
TTGAGTACTAACGGGGGGAAAATCATGGGGAAAATACTTAAAATGTCTAA82911.4629154205153994No Hit
TTGAGTACTAACGTCACTAATTGTCAGGGAAATGCAAATATAAAACACAG72151.2730593123891698No Hit
TTAAGATCCTATCTGAAGTTGAAGTGTATGGGTCACACGGCCGGCTTCAA71741.265825018306293No Hit
TCAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCT63621.1225507062259041No Hit
TCAAGAACCCCCATCCGTCTCCCTCCCTGCCTTACTGCTTCCACTCCTGC61761.0897317135572435No Hit
TCGAGCATACAAAATAGGGAAAAGCACAAATCTGATAAGAGAAATACCCT54000.9528094645740148No Hit
TCAAGGACGTGAAGCAGTTCTGACAACCTGGGACCTCTTAAAGAGCCATT49080.8659979355794933No Hit
TTGAGTAACACAAACCAACTAACTTACAGATAGGCATTACCTTTTGGATT46340.8176516775622193No Hit
TTAAGTGGTTTGAAGAACAGTAGGGAAGAGAACTATGGCTTGCCTTCCCT45850.809005813902196No Hit
TTGAGTGGTTCTCTATGTGTACATTGTCTTTGGAATGATTGAGAGGTGGA43600.7693054195449452No Hit
TTGAGTCTTTGAGAACAGTTTTTTACTCCTTTATACCATTTTTTGTTTAG42300.7463674139163116No Hit
TTGAGTGGGGGTTGCACAGACCTGCTCATCTGCTCAGAAGAGGAGCTCAG35590.6279720156331331No Hit
TGAGTCTCTGCTTGTGATTCTTTGGCTTTGTATCTAGGAGTGGAAGCGCT35230.621619952535973No Hit
TGAGTGTTTTGGAGGGAAAAGCAGTTTGTAAATGTTTGTTGAATGACAGA31810.5612753531129521No Hit
TCAAGGCCCATGGTCCGTGCTCGCTTCCAGCTCCAGCTTCCCGCTTGGGA29380.5183989272071213No Hit
TCAAGGCATTTTGCTTTTTGACTTTCTGGAGGGTGAAAGAATGATAACAT27250.4808158872155908No Hit
TTGAGAAATCCCACAAGACCCTTGGCCTTACCCACCAGCAGACCAGCAGC27090.4779927480612974No Hit
TCGAGCTGTTGAGGCGTGTCAGGAAATCTAGCAACATCTGTATCCTCCCC26180.46193614412125383No Hit
TTAAGAATCTCATTTTTCAACTATAAAATTGATATATGGAGGTTAAACTT22860.40335600666966626No Hit
TTGAGTCCAGTCCTGGTTCTATCTCTTAATACCTGTACCGTTTTGTACAA20900.3687725520295724No Hit
TTAAGATTATCTCAGGCCCTGAACTTTTCAATTCACAAGTGAAATTCTAT20360.35924445738383226No Hit
TCAAGTGTGGCCCTAGCACCAAGGAAGCTTCATTAGTGATTTACCTACTA20290.35800933400382884No Hit
TCAAGGGGAAGGCTCCCCCCAGCATCTGCTCCCAACAGTTAGTCTGTTCC17620.31089819936655816No Hit
TCAAGTGTGTGACAGGCTGTGTGTGCATGTGAATGTGTGTGTTTATGTAC16110.28425482359791443No Hit
TTAAGAAGACAGAAGGGCAAAGCCACAGGTGGTAAAGTCATGATTGTTGA16020.2826668078236244No Hit
TTAAGTTCCATCAAGTTCAAGACACTTTTATAAGTGGTACTACCAGCCAC14420.2544354162806906No Hit
TCAAGCATGTGCACACACATGTATGCACACCCATGCATGCACACACACAA13480.23784947374921703No Hit
TTAAGTTAAAAAAATAAAGTTCACATTTTTTATGTTCATTATGTAATTCA13470.2376730275520737No Hit
TGAGTGGTTCTCTATGTGTACATTGTCTTTGGAATGATTGAGAGGTGGAG13460.23749658135493035No Hit
GCCGCCTTACACTGACTTGAGTCTTTGAGAACAGTTTTTTACTCCTTTAT13390.236261457974927No Hit
TGAGTACTAACGGGGGGAAAATCATGGGAAAAATACTTAAAATGTCTAAA13190.23273253403206026No Hit
TAGAGTGTTTTGGAGGGAAAAGCAGTTTGTAAATGTTTGTTGAATGACAG12700.22408667037203683No Hit
CAAGGATTGGAGGTGGCAGCCCCACCTGGCAGACACCATCGTATATGCCT12270.21649948389487333No Hit
TCAAGTCTTCACAAGCGCTGATCATTTTTACCACCTGTAAGATACCTCTT11910.21014742079771329No Hit
TTGAGTACTAACGAGGCCAGGGATGTGGTATCCAGTTTACCTGTGAACAG11150.1967375098148197No Hit
TTGAGTACTAACGGGTTGCACAGACCTGCTCATCTGCTCAGAAGAGGAGC11050.19497304784338634No Hit
TTGAGTACTAACGCTTCTTAAAATTCAGTAGGTAGTGGCGATTTTGCATG11000.19409081685766968No Hit
TGAGTACTAACGGGGGGAAAATCATGGGGAAAATACTTAAAATGTCTAAA10800.19056189291480297No Hit
GCCGCCTTACACTGACTTGAGTGGTTCTCTATGTGTACATTGTCTTTGGA10320.1820924754519228No Hit
TGAGTGGGGGTTGCACAGACCTGCTCATCTGCTCAGAAGAGGAGCTCAGA10260.1810337982690628No Hit
TTGAGTACTAACGGAAACAGCTGAATACAGCAAGAAACACACGCTTCTTA10190.17979867488905946No Hit
TTGAGTACTAACGTTAAAGTTCAAACTCTTTGTGGAAAGAGGATTCATAG10110.17838710531191276No Hit
TTAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAACTCCAGATCCATCCT9990.17626975094619274No Hit
TTGAGTTCTAACGCAGGGCAAATAAGCCATAACAAGAAAAGTACAATGGT9890.17450528897475936No Hit
TTGAGCATCCCAAAATGTTGCCATGATCTTTGCTCTTGACTGGTCCGCAT9830.17344661179189935No Hit
GCCGCCTTACACTGACTTGAGTACTAACGTCACTAATTGTCAGGGAAATG9820.17327016559475603No Hit
GCCGCCTTACACTGACTTGAGTCCAAGGCCTTGTTAGTAAATGTGGAAGC9530.16815322587759926No Hit
TTGAGTTCAAATGTGGCAGTGTTCCAGAAGTAGTACTGTGCTGCATATCA9300.16409496334330254No Hit
TTGAGTACTAACGCTGGGCAAATAAGCCATAACAAGAAAAGTACAATGGT9290.1639185171461592No Hit
TTAAGAAAGCACTTAGGCCTCTCCGAGACCGTTTTTCTCTTCTGTAAAAT9190.16215405517472584No Hit
GCCGCCTTACACTGACTTGAGTGTTTTGGAGGGAAAAGCAGTTTGTAAAT9190.16215405517472584No Hit
TTGAGTCTTTGGAGAGGTGAAGAAGTAACTCTTAGCAATCCAAAAGGTAA9070.16003670080900584No Hit
TCAAGGCTCTTTGTTGCGGCCGGGCGCCGTGGCTCACGCCTATAATCCCA9030.15933091602043245No Hit
TGAGTAACACAAACCAACTAACTTACAGATAGGCATTACCTTTTGGATTG8750.15439042250041907No Hit
GCCGCCTTACACTGACTTGAGTCTCTGCTTGTGATTCTTTGGCTTTGTAT8450.14909703658611898No Hit
TTGAGTTCTAATTTGGTTGTGCTGCCGTCTAAGAGACTGTTTGTTATGAT8350.14733257461468563No Hit
TTGAGTACTAACGGCCAGGGATGTGGTATCCAGTTTACCTGTGAACAGCA8140.14362720447467556No Hit
TTAAGCCAAAGCCCACGGCAATTCTGGCTGCTTTCTCCTGAGGACCACAT8030.14168629630609886No Hit
TTGAGAGGGTGACATTTGAGCTGGTTTTGGGGTAACAGTTCACTAAAGAG7940.14009828053180884No Hit
TTGAGTACTAACGTGCCCTAAAAACAAAACAAACAAAAATCTTGAACTAA7920.13974538813752216No Hit
TTGAGTACTAACGGTTTGGATATAGTTAGTTAAGAAGGTGAGAGCTCAGT7910.13956894194037883No Hit
TTGAGTACTAACGGGGGGGAAAATCATGGGAAAAATACTTAAAATGTCTA7770.13709869518037213No Hit
TCAAGAACCCCCATCCGTCTCCCTCCCTGCCTTACTGCTTCCCCTCCTGC7500.13233464785750204No Hit
TAGAGTCTCTGCTTGTGATTCTTTGGCTTTGTATCTAGGAGTGGAAGCGC7370.1300408472946387No Hit
TTGAGTACTAACGCGGGGCAAATAAGCCATAACAAGAAAAGTACAATGGT7340.12951150870320868No Hit
TTGAGTACTAACGCAGGGCAAATAAGCCATAACAAGAAAAGTACAATGGT6990.1233358918031919No Hit
TTAAGCTGCTGGATCTGTGGGGAAAACAAACATCTGAGGTCCAGAATTCG6890.12157142983175856No Hit
GCCGCCTTACACTGACTTGAGTAACACAAACCAACTAACTTACAGATAGG6860.12104209124032854No Hit
TTGAGTCCTGTTTGGAGGACAGCTGCTCCCACATCTGGGGGCCTCTGGAT6570.1159251515231718No Hit
TTGAGTACTAACGTGAAATTGTTCAACTGACATGTTGGGGCCTTAGCGGC6130.108161518848865No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT5940.10480904110314161TruSeq Adapter, Index 7 (97% over 38bp)
TTGAGTACTAACGCACGCTTCTTAAAATTCAGTAGGTAGTGGCGATTTTG5880.10375036392028161No Hit
TCGAGCTCCTACCTTTTCAAGTGTTCCGTTCCCTGGCAGAGAGACCAGCA5870.10357391772313827No Hit
TTGAGTACTAACGCCGGGCAAATAAGCCATAACAAGAAAAGTACAATGGT5830.10286813293456493No Hit
TTGAGTTAGGGAGTTACTGATCTCTCTGGTAACTGAACAGGTTTAGAAGT5790.1021623481459916No Hit
GTGAGTCTCTGCTTGTGATTCTTTGGCTTTGTATCTAGGAGTGGAAGCGC5730.10110367096313157No Hit
TCAAGGGAAGTGGTGGGTAACGACAATATTGTCATAAATAGACTCACAGG5670.10004499378027155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGT151.2641068E-4143.999749
CGCCTTA38850.0142.702453
CCGCCTT39100.0141.618382
TCAAGGA33400.0139.713031
CTTACAC40400.0137.049246
TCGAGCA6000.0136.823881
GCCGCCT40450.0136.725981
CCTTACA40600.0136.551475
TTACACT40550.0136.542287
TAGAGTA5300.0135.872791
ACACTGA41050.0134.002189
GGATTGG28450.0132.864435
GATTGGA28850.0131.02236
TACACTG42700.0130.173058
GATCCTA8900.0127.8199845
CGAGCTG3550.0127.785682
TTGAGTG53800.0126.356641
CGAGCAT6450.0126.150432
CAAGGAT30450.0125.3310242
ATTGGAG30250.0125.196477
Produced by FastQC (version 0.11.2)