FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_428_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_428_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3183139
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG74750.23483108968851188No Hit
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC69330.2178038722154452No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC40340.12673024960581364No Hit
CTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAAGCT31970.10043545066677892No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA84650.0118.090341
TAGTAAC86200.0115.6871642
GTAACAA90800.0112.99474
CGCACTT26250.0105.3248755
AGTAACA107100.095.932013
TAACAAG110800.092.923455
AAGAGCG17050.090.796779
GCACGCA29650.090.578852
AGCACGC30600.086.8474041
CTCGCTC26950.084.1551743
ACGCACT35800.080.245174
TCGCTCT29000.078.454554
ACAAGAG134650.076.0413067
ACGTGCA26900.074.678332
AACAAGA141400.072.56416
CAAGAGC145500.070.370878
CACGCAC38650.070.043433
GAAGCGG16000.069.769171
CGCTCTG32350.069.66255
AAACACG47150.066.4257665
Produced by FastQC (version 0.11.2)