FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_412_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_412_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855516
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC10280412.016607521075No Hit
TTGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA296033.4602508895216455No Hit
TTAAGAGCACACAAGAAGCTATAAACAAGATAACAAAAAATCTCAACTCT229722.6851631062423147No Hit
TTAAGATTCGAGAAGGCAGAATAATAAAGGAAATATTTCCAACAGGGAGA162941.9045815624722389No Hit
TTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGGA146401.711247948606455No Hit
TCAAGAATCATGTTCCTCTGATCAGTATTTAGTATAGATCTGTTCCTTTT125271.4642625035650998No Hit
TTGAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAA123281.4410016878702443No Hit
TTAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAACTCCAGATCCATCCT116211.3583615034669136No Hit
TCAAGCATGTATGCCAGTACAATGGGAGTTATCATCGTTCCTTTCAATTT65860.7698277998307455No Hit
TTGAGTAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCCGTGCCAAC64410.7528789642741924No Hit
TCGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG59190.6918631562706016No Hit
TCAAGTGCCAATAGATGCTCATCTTCACTGTTTATTATTCCTTCGTTGGA54120.6326006760832059No Hit
TTGAGTAGCTGGCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCA53570.6261718074238237No Hit
TTAAGGAATCTGCATTCACTAACCCCTGAAGCTGGGCCATCAGTTATCAT49280.5760266318806428No Hit
TTGAGTACTTTTCTAGACATTCTTTGGCTAATGTCTTTGGTTTTTTGGAC48360.5652728879413126No Hit
TCAAGAAAGGGATAATGGCATTTCCTATTTCTTAATCACAGGTTTGTTAG48280.5643377797726752No Hit
TTGAGTAGCTGGCAAAAGCCATAGGAAATTGCCCAATATGGGTGAAAACA44550.5207383614099561No Hit
TTGAGTAGCTGGCACAGAATTCAAGCCTAGATCAGCATTAAAATGCAAGG44150.5160628205667691No Hit
TTGAGAAAATACCATGCACAAGATTATTAAATCTTTCATGTCCTCCTTCT42990.5025037521215266No Hit
TCAAGGGCCTTTACTTTTGCAATTTTCCCTGCTTGGAGCAATCTTCCTCC41690.48730824438116876No Hit
TGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGAC41480.4848535854384956No Hit
TCAAGATGATGAATGACTCAATGGCTAAGAAAACCAGTGGAAATGCTTTC38590.4510728028464693No Hit
TTGAGTTGTTGCCCTTTTGTTTCTCACCACCAAAGCTAAGGAGCAATGGG38590.4510728028464693No Hit
TTGAGTTGTGTCCAGGTTTTGCGTGGCCATGGAAAATCAGGTGAAGGACA37910.4431243834130513No Hit
TTGAGTACCACCTTCCAATCTTGGATGCCATTCTTTGATGAACAAATCCT36280.4240715544770642No Hit
TTGAGTATCTATCACAGTCTTATTTGCAATTTCTACAGCTAGCTCCAATG35800.4184609054652397No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTGTAGAATTCGCCCAGTTTCAC34910.4080578270891485No Hit
TTGAGTAGCTGGCAGGAGCAAAGTAATAAAAGGGTCCTTGCCTTTAATTG33330.3895894407585598No Hit
TTGAGAGGGAATTAGTTGCCAGGAGAAGGTTCCTGCCCGTGGCAGGAGCA31780.37147171999121No Hit
TTGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG29340.34295092084776907No Hit
TAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCCG27900.32611897381229576No Hit
TCGAGCTGGATGAGAAAGTGGATGATCTCAGAGCTGACACTATAAGTTCA26650.31150790867733624No Hit
TTGAGTGCCAAGTCGTTTTCCCACCATCATGTACCATTTCTATCCCAATA26100.3050790400179541No Hit
TCAAGAGCATGGAATAGAGACTCCTAAGTATCTGGCTGATTTGTTTGATT24010.28064933911230183No Hit
TTGAGGAACAATGCTACGCTAAGTGTTGCAACCTTTTTGAGGCCTGTTTT23890.27924667685934573No Hit
TTGAGTAGCTGGCTGGAGCAAAGTAATAAAAGGGTCCTTGCCTTTAATTG23420.27375291636860094No Hit
TCAAGTAGCTGGGACTATAGGTGCTGGCCACCATGCCCATGTAATTTTTT21920.25621963820664956No Hit
TTGAGTACTCAAATAAGGGAAAACAATATGTCTCTGACATCACAGGATGT20730.24230990419816814No Hit
TTGAGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCTTGC20060.23447837328582985No Hit
TTGAGTGTGGATGCTTAAAAAACACTTCCTCTAGCCGAATGGTATGGTGT19730.23062105209020056No Hit
TCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGTCTCTTAATTCCT19420.22699750793673057No Hit
TTAAGAGTACAGGTTGAGACCATCCTGGCTAACACGGTGAAACCTCATCT19370.22641306533133218No Hit
TTGAGTAGCTGGCGAAAGCCATAGGAAATTGCCCAATATGGGTGAAAACA19150.22384151786757933No Hit
TTGAGTTGTTGGCTGGGGCACTCCCTGTCCAGCTTCCTTGCTGACCCCCA17360.20291847259431733No Hit
TTGAGTTTTGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCAT15880.1856189714745253No Hit
TTGAGTAGAAGTAACGATCTCTTTGATCAATGGGGGTATGAGGAATCACC15660.18304742401077245No Hit
TCAAGACTTCAGGCCTGGGCCTGCAGCCCCTTTCCATGCTGACAAGAAGC14910.17428078492979676No Hit
TTGAGTAGCTGGCAAGAGCCATAGGAAATTGCCCAATATGGGTGAAAACA14890.1740470078876374No Hit
TTGAGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAGTCATCC14240.16644925401745847No Hit
TTGAGTTTCCTTTGATTTCTCCGAATCTATGGGGGCTAATTAGGAGTGCT14190.1658648114120601No Hit
TTGAGTAGCTGGCAAAGCCATAGGAAATTGCCCAATATGGGTGAAAACAC13680.15990349683699662No Hit
GCCGCCTTACACTGACTTGAGTAATAGAGCAGTATAGTATGGTTATCCAA13450.15721506085216408No Hit
TTGAGTATCCGTGCCAACCTGCAATCATTCCTTCCCATCCTCCTTCTAGG13140.15359151669869414No Hit
TGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGGAA13090.15300707409329575No Hit
TCAAGCTCCCCTCCAAGCTTCTGTCTTTAATTCGATGGTCCACCTGTATT12760.14914975289766644No Hit
TCAAGGGTGTTGAAAAGTGAATTTACAGAGTTAAATTAAAGAGGGTGTGA12690.1483315332501087No Hit
TTAAGGTCTGCAACAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC12270.14342221536476232No Hit
TTGAGTAGCTGGCGGGAGCAAAGTAATAAAAGGGTCCTTGCCTTTAATTG12010.14038311381669075No Hit
TTGAGTAGCTGGCAAGGGTTTCCATGTTCCAGCAAAGGAACAGGTAGAAG11650.13617512705782242No Hit
TTGAGTAGCTGGCTTCGGAGCTGCCTATGAAGACCTGAGAGTTTTGTCTG11220.13114892065139636No Hit
TTGAGTAGCTGGCATATCCCAGAAATAGTGTTGAAGTACAATCTAATGGA11170.13056447804599797No Hit
TAAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC11110.12986314691951992No Hit
TTGAGTACTATGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAA11100.12974625839844023No Hit
TTGAGTCCCAGCTCTGTCACTTGCTACTATGACCTTGGATGTCATATAGC11010.12869426170872314No Hit
TTGAGTAGCTGGCTAAAGCCATAGGAAATTGCCCAATATGGGTGAAAACA10970.12822670762440447No Hit
GCCGCCTTACACTGACTTGAGTATTTCGTTGTGGAGTTCATCCATGGCAC10940.12787604206116543No Hit
TTGAGTAGCTGGCAGAAGCCATAGGAAATTGCCCAATATGGGTGAAAACA10720.12530449459741255No Hit
TTGAGTCCGGGCAAGATAAACGAGGGTATGTCCACTCCGGTTCTGGGAGA10590.12378494382337676No Hit
TCAAGGGGAGGTCAATGTGACTGGTGTGATACCACTAACAACAACACCAA10260.11992762262774746No Hit
TTGAGTAGCTGGCAAATGCCATAGGAAATTGCCCAATATGGGTGAAAACA10070.11770674072723362No Hit
TTGAGTGGCAGGAGCAAAGTAATAAAAGGGTCCTTGCCTTTAATTGGTGA9970.11653785551643686No Hit
TTGAGCCTTTGTGTCCAAAGCAAGAACTACTTCTGGATTTTTAGTCAAAT9830.11490141622132141No Hit
GCCGCCTTACACTGACTTGAGTAACTGCCACTCCATGTGCTCCGTGAGAT9640.11268053432080755No Hit
TTGAGTAACAGGCAAAGATAATGAAAGTCTTAAAGAGTGCAATATTAGTA9560.11174542615217016No Hit
TTGAGTCCTGGGGTGAGTCCCTCAGCGCGTCAGAAGGTTATAGAGCACCA9520.11127787206785145No Hit
TAGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA9370.1095245442516563No Hit
TTGAGTAAGTGCAAGGGGAGCAGTAAGTGAGGGGACAGTAATCAAAAGGT9340.1091738786884173No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATAAGA9330.10905699016733761No Hit
TTGAGTAGCTGGCTGCGTTAACAGGCACAGAATTCAAGCCTAGATCAGCA9320.10894010164625793No Hit
TTGAGTATCAGCAGGGGAACGGTAAATGAGGGGCAAATAGCAAGTGCAAG9140.10683610826682376No Hit
TTGAGTAGCTAGCATCAATGTTACAGCCAAACTAGAAGCAGCAGTTGAGT9050.1057841115771067No Hit
TCAAGACACTAGGATGTTCCAAGGAGTGAGGGGTGAAATAAATTTTCTTA9050.1057841115771067No Hit
GCCGCCTTACACTGACTTGAGTAAATTTCCTTTGACATCTGCATCACGTC8920.1042645608030709No Hit
TTAAGTGTTGCATTGAAGAGATTTTATTTTCCTCTATAGGTGGTCAGGGG8680.10145923629715867No Hit
TTGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGAGGGTATGT8610.10064101664960094No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTG202.2038694E-6144.015966
CTAGGCG202.2038694E-6144.015969
TCGAGTT1000.0144.007551
GCCGCCT49400.0141.966951
CCGCCTT49650.0141.67892
CGCCTTA49700.0141.536363
CCTTACA50100.0140.11895
TTACACT50350.0139.296487
TTAAGGT120150.0138.014711
TTAAGGC14000.0137.835781
ACACTGA51100.0137.111089
TAGAGTA10400.0137.084091
TAAGGTC121850.0136.199422
AAGGTCT123550.0135.607423
ATTCGAG19550.0135.544436
CGAGAAG19700.0134.512379
TCGAGAA19700.0134.512378
CTTACAC52250.0134.506766
GATTCGA19800.0133.817375
TTCGAGA19900.0132.798637
Produced by FastQC (version 0.11.2)