FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_412_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_412_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2975328
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT56260.18908839630454188No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC56200.18888673786553953No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA52250.1756108906312178No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT42290.14213558975682683No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC34940.11743243097903829No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT34640.11642413878402649No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG33250.1117523849471386No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA32540.10936609341894406No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC6950.088.0839541
GCACGCA7550.082.0185242
GAAACAC24550.080.9447864
AAACACG26500.074.988475
AACACGA20450.072.1758356
GCATACG300.001990028471.99989
AACACGT7450.070.550136
GTAACAA45300.067.867364
CGGTGCA3950.067.447382
ACGCACT9650.065.657854
CACGCAC9550.064.839693
CGCACTT9500.063.662985
GCGGTGC12150.061.64811
ACGTGCA11350.060.90262
ACACGAG24350.060.6158457
TAACAAG51350.059.5908785
CTGTACG502.0808919E-457.599849
ACAGCGT2400.053.9998474
ACACGTG9800.053.63257
GAAGCGG10550.050.517511
Produced by FastQC (version 0.11.2)