FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_394_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_394_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546786
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTCTCGTTGTCAGAGTGGAACCCCCAAACGGTAATTTGGTCTTCTC447198.178519567070115No Hit
TTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCC298765.463929215451749No Hit
TTAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAACTCCAGATCCATCCT262334.797672215455407No Hit
TCAAGGGGAGGTCAATGTGACCGGTGTAATACCACTGACAACAACACCCA231804.2393184902320105No Hit
TTGAGTTACACGTGGGTGCTTTCCTATAATGCACGATAGAACAAAAATTA189903.4730223524376997No Hit
TCCAGGAGGACCCTACAAAATTGGAACCTCAGGGTCTTGCCCTAACGTTA135862.484701510280073No Hit
TTGAGTAGATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA121312.2186010614756047No Hit
TGGAGCATCTTCTGCATTGATAACGTTGTGAGTTGATAACCTGATATGTT113142.0691824589510337No Hit
TTGAGTAACCCATCCCCGGAAAGAGCAACCACAAGCAGTGAAGATGATGT113082.0680851375126648No Hit
TTGAGTTACACGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGC75621.3829907861576558No Hit
TGAGTCTCGTTGTCAGAGTGGAACCCCCAAACGGTAATTTGGTCTTCTCC60601.108294652752631No Hit
TCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAGC49620.9074848295311145No Hit
TTGAGTTATCCGGCCCCAAGTGGCGTTGTCAAGTCTCATTTTTCTGAGAA43010.7865965844041362No Hit
TTGAGTTACACGTGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATAT41490.758797774632123No Hit
TTGAGTCCCCATAGAGCTTTGCCATTTGAGTCTCGTTGTCAGAGTGGAAC38420.7026514943689122No Hit
TTGAGTAGCAGTTTTGACGACATGTGGTGAGTTTGACGATGTTATCCCAG36180.6616848273364717No Hit
TTGAGTTACACGTCTCTGGCATTCTTTTTCTTCTGGAACTCGGTTTTCTT35520.6496142915144133No Hit
TCAAGAATCATGTTCCTCTGATCAGTGTTTAGTATAGATCTGTTTCTTTT31090.5685953919815065No Hit
TTGAGTTACACGTGCAGAGACAGAGGTTAATCCAGGGCTGCTGGAACAGC30600.5596339335681602No Hit
TTAAGATTCGAGAGGGCCGAATAATAAAAGAAATATTTCCAACAGGAAGA25800.47184821849864483No Hit
TTGAGTTACACGTCCACCAAATCTCATTTTGCAAATCTCAAAGGAACAGA25280.46233809936611403No Hit
TTGAGTTACACGTGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATG23500.4297842300278354No Hit
TTGAGTTACACGTAGACCAATATAACATAATAAGAAAATTCAATACATCA23110.42265164067843725No Hit
TTGAGTTACACGTACCAAATCTCATTTTGCAAATCTCAAAGGAACAGAAA21870.39997366428547915No Hit
TTGAGTTACACGTAGCCTTGGGCAGACCAAAATGCACAGGGAAAATACCC21800.39869345594071537No Hit
TTGAGTTACACGTTTGGGGGTTCCACTCTGACAACGAGACTCAAATGGCA20650.3776614617053107No Hit
TTGAGTTACACGTTAGAACAAAAATTAGACAGCTGCCTAACCTTCTCCGA19900.3639449437256989No Hit
GCCGCCTTACACTGACTTGAGTCTCGTTGTCAGAGTGGAACCCCCAAACG17600.32188095525488947No Hit
TGAGTTACACGTGGGTGCTTTCCTATAATGCACGATAGAACAAAAATTAG17070.3121879492159638No Hit
TAGAGTCTCGTTGTCAGAGTGGAACCCCCAAACGGTAATTTGGTCTTCTC16590.30340937770901233No Hit
TTAAGAAATCTGCATTCACTAACACCTGAAGCTGAGCCATCAGTTATCAT15820.2893270859166109No Hit
TTGAGTCACTGAGGATAAATCATCTGCTTATTTTAAAATGTGTAGTCCAA15530.284023365631161No Hit
TGAGTAGATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGAC14990.27414747268584055No Hit
CAAGGGGAGGTCAATGTGACCGGTGTAATACCACTGACAACAACACCCAC14730.2693924131195751No Hit
TTGAGTTACACGTGGTGCTTTCCTATAATGCACGATAGAACAAAAATTAG14640.2677464309620217No Hit
TTGAGTTACACGTCTGGCATTCTTTTTCTTCTGGAACTCGGTTTTCTTGT14600.2670148833364424No Hit
TTGAGTTACACGTGCTTTCCTATAATGCACGATAGAACAAAAATTAGACA14290.2613453892382029No Hit
TCCAGGAGGCCCCTACAAAATTGGAACCTCAGGGTCTTGCCCTAACGTTA14270.2609796154254132No Hit
TTGAGTTACACGTCACCAAATCTCATTTTGCAAATCTCAAAGGAACAGAA13290.24305669859872053No Hit
TTGAGTTACACGTTTGGGGGTTCCACTCTGACAACGAGACTCAAAGATCG12970.23720431759408617No Hit
TTGAGTTTCCTTTGGTTTCTCCGAATCTATGAGGGCTAATTAGGAGTGCT12810.234278127091769No Hit
TTGAGTTACACGTTTTGTCTGCATTAACAGGCACAGAATTCAAGCCTAGA12290.22476800795923815No Hit
TTGAGTTACACGTAGAACAAAAATTAGACAGCTGCCTAACCTTCTCCGAG12280.22458512105284334No Hit
TGAGTAACCCATCCCCGGAAAGAGCAACCACAAGCAGTGAAGATGATGTC11500.2103199423540471No Hit
TTGAGTCTCGTTGTCAGAGTGGAACCCCCAAACGTGTAACTCAAAGATCG11000.20117559703430593No Hit
TCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTC10280.18800773977387863No Hit
TTGAGTTACACGTGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTT10250.18745907905469414No Hit
TAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCCG9740.17813184682855815No Hit
TTGAGTTACACGTTGTGATCTATTTCTTCCTTCCCTTCTTTGACATCTCT9690.17721741229658405No Hit
TTGAGTTACACGTCTTCGAGGTTTTGCAGGTAATTCCGATACCTACACAT9330.17063348366637038No Hit
TTGAGTTACACGTTATCAATGCAGAAGATGCTCCAGGAGGACCCTACAAA9150.16734151935126357No Hit
TTGAGTTACACGTGCCTCCTGTTTTGTTGTGATCTATTTCTTCCTTCCCT8850.16185491215941886No Hit
GTGAGTCTCGTTGTCAGAGTGGAACCCCCAAACGGTAATTTGGTCTTCTC8750.1600260430954706No Hit
TTGAGTTACACGTGCTGAGACAGAGGTTAATCCAGGGCTGCTGGAACAGC8590.15709985259315346No Hit
GCCGCCTTACACTGACTTGAGTCCCCATAGAGCTTTGCCATTTGAGTCTC7980.1459437513030692No Hit
TTGAGTTACACGTCCCACCAAATCTCATTTTGCAAATCTCAAAGGAACAG7730.14137157864319863No Hit
TAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAACTCCAGATCCATCCTC7710.14100580483040898No Hit
GCCGCCTTACACTGACTTGAGTTACACGTGGGTGCTTTCCTATAATGCAC7670.14027425720482967No Hit
TTGAGTTACACGTTTTGAAGCAAACAACAGTAGACCAATATAACATAATA7440.13606785835774873No Hit
GTTGTCAGAGTGGAACCCCCAAACGGTAATTTGGTCTTCTCCTTCTGTAC7340.13423898929380051No Hit
TCAAGAAGGAAAACTAGTAAAAGGATCCTTTGGAAAAGCACTAAGAGTAA7150.13076413807229886No Hit
CCAGGAGGACCCTACAAAATTGGAACCTCAGGGTCTTGCCCTAACGTTAC7150.13076413807229886No Hit
TTGAGTGCTCTTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGG7140.13058125116590405No Hit
GCCGCCTTACACTGACTTGAGTAGATTTCCTTTGACATCTGCATCACGTC6890.12600907850603343No Hit
TTGAGTTACACGTACTTGAATATATGTTAAACATAGCTCTCTGTACCAGA6790.1241802094420852No Hit
TTGAGTTACACGTTAACCCTATTTCTCACATCAGGGGGAGTATTATTATC6720.12290000109732144No Hit
GCCGCCTTACACTGACTTGAGTAACCCATCCCCGGAAAGAGCAACCACAA6270.11467009030955437No Hit
TTGAGTCTACCAAGTGCTGGCTGCTGAATCAGGCTCAGGCACACACAGAT6210.11357276887118542No Hit
TGAGTTACACGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCA6150.1124754474328165No Hit
TAGAGTTACACGTGGGTGCTTTCCTATAATGCACGATAGAACAAAAATTA6060.11082946527526309No Hit
TTGAGTTACACGTGACCGGTGTAATACCACTGACAACAACACCCACCAAA5970.10918348311770966No Hit
TGAGTTACACGTCCACCAAATCTCATTTTGCAAATCTCAAAGGAACAGAA5930.10845193549213039No Hit
TGAGTCCCCATAGAGCTTTGCCATTTGAGTCTCGTTGTCAGAGTGGAACC5750.10515997117702355No Hit
TTGAGTTACACGTCTGGACGTAGCCTTGGGCAGACCAAAATGCACAGGGA5660.10351398901947013No Hit
TTGAGTTACACGTGCAGATACAGAGGTTAATCCAGGGCTGCTGGAACAGC5630.10296532830028568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGTC202.1983997E-6144.078411
CACGCGC151.2640959E-4143.999369
ACTCGTG100.007238302143.999368
ACACTAG253.8657163E-8143.999366
GGTCTCG253.8657163E-8143.999364
GCTCGTC100.007238302143.999366
GGGGCTC100.007238302143.999363
CGCCTTA30650.0143.059723
GCCGCCT30750.0142.907041
AGAGTTA5650.0142.738082
CCTTACA30700.0142.357675
TTAAGGC28750.0142.073841
CCGCCTT31050.0141.69352
CTTACAC30950.0141.44046
TAGAGTT5850.0140.384111
TTAAGGT34050.0138.366041
TTACACT32050.0136.810647
GCCTTAC32250.0135.738944
TAAGGTC34950.0135.565442
TACACTG32550.0135.151478
Produced by FastQC (version 0.11.2)