FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_394_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_394_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3169882
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT104600.32998073745331846No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC67070.21158516310701786No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA59750.18849282086841088No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT57710.18205725008060236No Hit
GTATTAATCCACCTTTGTCGGCTCCATTTAAATCATTCAACCTAAAAGAG56810.17921802767421627No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA55670.17562167929279388No Hit
ACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA51860.16360230443909268No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT50470.15921728316700748No Hit
GATCTATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGC48870.1541697766667655No Hit
CCATAGCATTCTGTGAGGCTGCTTGAAAAAGACCTGGGTGTTCTTCATCC48350.15252933705418686No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC47980.15136210117600593No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC47030.14836514419148727No Hit
ATATAAATCCTTATTTTCTCTTCATAGATGTGCCCGTACAGGCAGCAATT42110.1328440617032432No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT39590.12489423896536213No Hit
ACACAATAGACACCGTGATCAGAACGCATGAGTACTCAAACAAGGGGAAA37400.11798546444315593No Hit
GCTTGGAGGCCTGGGTGCTACCCACAGTGCTACATTGCCACCCAGTGCTG36700.11577718034930005No Hit
TCTGCATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA36570.1153670704461554No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC36300.11451530372423957No Hit
ATGGTAGATCCAACAAATGGACCATTACCCGAAGATAATGAGCCGAGTGC34990.11038265777716648No Hit
ATTCTATCCAAAGCCTCTAAAACGCAATCTAATTGCGCATAGGCACTCGG34970.11031956394591344No Hit
TTTGTGTATCGCATGTATTCAATGATGACCAATAACCCCATAAACATCTT34310.10823746751456363No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT33750.10647084023947895No Hit
CTGTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTC33670.10621846491446685No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG31740.10012991019854997No Hit
GTTTAGTATAGATCTGTTTCTTTTGGTTCTCCAACTATGAGTTCCAGACA31700.10000372253604393No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC26750.083.978542
GAAACAC40800.075.176154
AACACGA30150.072.5996
TAGAAAC47550.072.53082
GTAACAA29100.072.494534
ACACGAG32150.068.0827267
CTGTCGT3050.066.123111
GAAGCGG14600.065.61361
GTAGAAA53250.065.466761
CGGTGCA4250.062.6830752
AAACACG49950.060.9727135
AGTAACA39400.058.2941673
TAACAAG38400.055.8747635
CGTCAGA553.3317925E-452.3642352
AAGCGGA13800.052.193451
ACGAGCG3200.051.751419
CACGAGC43700.049.923568
ACAAGAG42800.049.1228377
GTCGTCC4550.049.0547373
GACACGA3800.043.580146
Produced by FastQC (version 0.11.2)