FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_379_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_379_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650832
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCC6728910.338920028517345No Hit
TCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAGC474457.289899697617819No Hit
TTGAGTCCCCATAGAGCTTTGCCATTTGAGTCTCGTTGTCAGAGTGGAAC283204.351353344641935No Hit
TTGAGTTTCAAAGCATCCATTCCCTATCTCTACAGCAGAGGGGCCCAGCA135282.0785701993755685No Hit
TTAAGATTCGAGAGGGCCGAATAATAAAAGAAATATTTCCAACAGGAAGA124171.9078656243085774No Hit
TTGAGTGCCAAGTCTCTTTTCCACCATCATGTACCATCTCTATCCCAATA121621.8686850062688987No Hit
TCCAGGAGGACCCTACAAAATTGGAACCTCAGGGTCTTGCCCTAACGTTA119881.8419499963124124No Hit
TTAAGAAATCTGCATTCACTAACACCTGAAGCTGAGCCATCAGTTATCAT103911.5965717727462696No Hit
TCAAGAAGGAAAACTAGTAAAAGGATCCTTTGGAAAAGCACTAAGAGTAA94911.4582872384885808No Hit
TCAAGAATCATGTTCCTCTGATCAGTGTTTAGTATAGATCTGTTTCTTTT87811.3491961059075153No Hit
TGGAGCATCTTCTGCATTGATAACGTTGTGAGTTGATAACCTGATATGTT85351.3113983332104138No Hit
TCAAGCCGTCCAAATCTTTGTCTTTGTCTGATCTTTAGTCCTAATGCAGC75181.1551368094992256No Hit
TTGAGTTTCAATACTCCATGAAGTCAGACCTGTTACATCTGGGTGCTTTC72271.1104248100892395No Hit
TTGAGTTCCAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTC63320.9729085232440936No Hit
TCAAGGGGAGGTCAATGTGACCGGTGTAATACCACTGACAACAACACCCA45230.6949566093861396No Hit
TCAAGACACTAGGATGTTCCAAGGGGTGAGAGGAGAAATAAATTTTCTTA39720.6102957445239324No Hit
TTGAGTCTCGTTGTCAGAGTGGAACCCCCAAACGGTAATTTGGTCTTCTC39110.6009231260909114No Hit
TTGAGTAGATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA34830.535161147577255No Hit
TCAAGATGATGAATGACTCAATGGCTAAGAAAACCAGTGGAAATGCTTTC34710.5333173537871524No Hit
TGAGTCCCCATAGAGCTTTGCCATTTGAGTCTCGTTGTCAGAGTGGAACC33990.5222545910465374No Hit
TTGAGTTCCAATGCAAAGGCCATAGGAAATTGCCCAATATGGGTGAAAAC33840.5199498488089092No Hit
TTGAGTTCCAATGCACGGTAGAACAAAAATTAGACAGCTGCCTAACCTTC27280.4191557882833051No Hit
TTGAGTTCCAATGGGGACAAAGGGAGTGGAGGCATCAAGGGAGGATTTGT27060.4157754996681171No Hit
TTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCT25380.3899623866066819No Hit
TTGAGTTCCAATGATGCACGATAGAACAAAAATTAGACAGCTGCCTAACC21850.33572411928116624No Hit
CAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAGCT21540.33096098532340146No Hit
TTGAGTTCCAATGCACGCTAGAACAAAAATTAGACAGCTGCCTAACCTTC19450.2988482434791159No Hit
TAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCCG19340.297158099171522No Hit
TTGAGTTCCAATGGTGCGCAAGTGGCAGGAGCAAGGTAATAAAAGGATCC19280.29623620227647074No Hit
TTGAGTTCCAATGCGCGATAGAACAAAAATTAGACAGCTGCCTAACCTTC18500.2842515426408044No Hit
TGAGTGCCAAGTCTCTTTTCCACCATCATGTACCATCTCTATCCCAATAC17060.26212601715957423No Hit
TTGAGTACTTTTCTAGACATTCTTTGGCTAATGTCTTCGGTTTTTTGGAC16410.2521388007965189No Hit
GCCGCCTTACACTGACTTGAGTTTCAAAGCATCCATTCCCTATCTCTACA15310.2352373577205792No Hit
TTGAGTTCCAATGCAGAAGATGCTCCAGGAGGACCCTACAAAATTGGAAC14810.22755488359515202No Hit
TTGAGCTAAAAAGAATATCAGGAAGAGGATTCAAAAATGATGAAGAAATA14810.22755488359515202No Hit
TTGAGTAGTAAAGCAGTATAGTATGATTATCCAATCCATCGTCATTTAAA14640.2249428423925068No Hit
TTGAGTTCCAATGATGGGGACAAAGGGAGTGGAGGCATCAAGGGAGGATT13910.2137264301693832No Hit
TTGAGAAAATACCATGCATAAGATTATTAAATCTCTCATATCCTCCTTTT13390.20573665707893896No Hit
TTGAGTTCATAGAAATGCTACACTGCTTACAAGGTGAAAATTGGAGACAA12630.1940592964082897No Hit
GCCGCCTTACACTGACTTGAGTCTCGTTGTCAGAGTGGAACCCCCAAACG11940.18345748211520024No Hit
TTGAGTTCCAATGCACGACAGAACAAAAATTAGACAGCTGCCTAACCTTC10760.16532684317919216No Hit
TTGAGTGCTCTTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGG10660.16379034835410675No Hit
TTGAGTTCCAATGCACGTTAGAACAAAAATTAGACAGCTGCCTAACCTTC10300.1582589669837992No Hit
TAGAGTCCCCATAGAGCTTTGCCATTTGAGTCTCGTTGTCAGAGTGGAAC9990.15349583302603437No Hit
TTGAGTTCCAATGGCCATAGGAAATTGCCCAATATGGGTGAAAACACCCT9970.1531885340610173No Hit
TCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCT9450.14519876097057305No Hit
TTGAGTTTGGTTTCAAAGCATCCATTCCCTATCTCTACAGCAGAGGGGCC9420.14473781252304743No Hit
TTGAGTAGCAGTTTTGACGACATGTGGTGAGTTTGACGATGTTATCCCAG9310.14304766821545345No Hit
TTGAGTTCCAATGCGAAGGCCATAGGAAATTGCCCAATATGGGTGAAAAC9130.14028197753029967No Hit
GCCGCCTTACACTGACTTGAGTCCCCATAGAGCTTTGCCATTTGAGTCTC9090.1396673796002655No Hit
TCGAACGATGTCTAAAACTGAAAGGATGGGGATGGGACTGTATGTCAAGT8970.137823585810163No Hit
TTGAGTTCCAATGCACAGGGAAAATACCCTCGGCAAGAGTTTCAATACTC8540.13121665806229565No Hit
GCCGCCTTACACTGACTTGAGTTTCAATACTCCATGAAGTCAGACCTGTT8510.13075570961477004No Hit
TGAGTAGATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGAC8470.13014111168473586No Hit
TTGAGTCCTAATGCAGCTCTTATTATGTCACAAGCTACATCACCTCCGTC8200.1259925756570052No Hit
TTGAGTTCCAATGTGCACAGATACTTATTTGGACACCCCCAGACCAAATG8080.12414878186690267No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTATAGAATTCGCCCAGTTTCAC7810.12000024583917202No Hit
TCGAGAGTACCACCTTCCAATCTTGGATTTCATTCTTTGATGAACAAATC7800.11984659635666348No Hit
TTGAGTTCCAATGCCCACCAAATCTCATTTTGCAAATCTCAAAGGAACAG7750.11907834894412075No Hit
TGAGTTTCAAAGCATCCATTCCCTATCTCTACAGCAGAGGGGCCCAGCAT7700.11831010153157803No Hit
TTGAGTTCCAATGAGGAGCTACATAGACAATATAGACCCAAAAGGAGCAA7640.11738820463652677No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATGAGA7630.11723455515401825No Hit
GCCGCCTTACACTGACTTGAGTGCCAAGTCTCTTTTCCACCATCATGTAC7510.11539076136391573No Hit
TTGAGTTCCAATGCATGATAGAACAAAAATTAGACAGCTGCCTAACCTTC7250.11139587481869362No Hit
TAAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCC7190.11047397792364236No Hit
TTGAGTTCCAATGTACGATAGAACAAAAATTAGACAGCTGCCTAACCTTC6940.10663274086092879No Hit
TTGAGTTCCAATGGGTGCTTTCCTATAATGCACGATAGAACAAAAATTAG6860.10540354500086044No Hit
CCAGGAGGACCCTACAAAATTGGAACCTCAGGGTCTTGCCCTAACGTTAC6700.10294515328072376No Hit
TAAGATTCGAGAGGGCCGAATAATAAAAGAAATATTTCCAACAGGAAGAG6620.10171595742065542No Hit
TGAGTTTCAATACTCCATGAAGTCAGACCTGTTACATCTGGGTGCTTTCC6510.10002581311306144No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGT100.007235218144.021971
CAACGGG100.0072385427143.999859
CGCCTGA100.0072385427143.999853
CGTACAC100.0072385427143.999856
CGGCTGC100.0072385427143.999858
CGCCGTA100.0072385427143.999853
GCCGCCT38400.0143.646911
CGCCTTA38550.0142.692463
AGAGTTC4150.0142.264912
CTTACAC38650.0142.136986
CCGCCTT38750.0141.955982
TTACACT38600.0141.948037
CCTTACA38950.0141.04225
CCGGGTT2300.0140.891071
TTAAGGT79650.0140.22481
TAGAGTT5000.0139.701311
TCAAGAC4100.0138.752871
TAGAGTC1250.0138.26111
CGGGTTA2400.0137.999852
AGAGTCC1200.0137.999852
Produced by FastQC (version 0.11.2)