FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_379_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_379_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2615684
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC112200.42895089773841183No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT108130.41339091419299884No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA88910.33991109017755966No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAATC54180.2071351126512224No Hit
AGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAAT53390.20411487014486462No Hit
CAACAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG42230.1614491658778354No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA38830.14845065382515624No Hit
CAACAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAA37960.14512456397638246No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT35420.1354139108546751No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT33940.12975573501997947No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC33540.1282264983078996No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA31970.12222424421298597No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC28810.11014327418755476No Hit
ACAACAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAA28410.10861403747547486No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT28340.1083464210508609No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG28040.10719949351680096No Hit
CACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG27150.10379694183242318No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT27080.1035293254078092No Hit
CACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA26810.10249709062715526No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC26620.10177070318891732No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT26580.10161777951770933No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA37200.0131.612764
CACGCAC5600.0113.142733
AGCACGC5800.0105.533271
GAAACAC19950.0105.022454
GCACGCA6100.0101.5080952
TAGAAAC23750.0100.041992
TAACAAG49350.099.209625
AAACACG22050.095.020315
CGGTGCA1450.094.344732
ACAGCGT1150.093.912954
ACGCACT7400.087.5674744
CGCACTT7450.086.013335
TAGTAAC56200.085.8362052
GAAGCGG5000.084.972911
CGGTGCG4450.084.1347352
CAGCGTT1200.083.999915
ACAAGAG59800.081.8728267
GAGCAGA3200.081.01231
ACGTGCA12000.080.9999162
AACACGT4900.080.816246
Produced by FastQC (version 0.11.2)