FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_361_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_361_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842996
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA9488811.256043919544103No Hit
TTAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC9347011.087834343223456No Hit
TTGAGAAAATACCATGCACAAGATTATTAAATCTTTCATGTCCTCCTTCT239052.8357192679443317No Hit
TTAAGAGCACACAAGAAGCTATAAACAAGATAACAAAAAATCTCAACTCT173392.0568306373932974No Hit
TCAAGACACTAGGATGTTCCAAGGAGTGAGGGGTGAAATAAATTTTCTTA134561.5962116071725132No Hit
TCAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCT130401.5468638048104617No Hit
TGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGAC110201.3072422644947308No Hit
TTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGGA103621.2291873271047549No Hit
TTGAGTGAGTTGCGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCA84901.007122216475523No Hit
TTGAGTGAGTTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCATTA82600.9798385757465042No Hit
TTGAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAA78270.9284741564610033No Hit
TCGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG72680.8621630470369966No Hit
TCAAGTCTTCACAAGCGCTGATCATTTTTACCACCTGTAAGATACCTCTT72050.8546897019677436No Hit
TCAAGATGATGAATGACTCAATGGCTAAGAAAACCAGTGGAAATGCTTTC59320.7036806817588696No Hit
TTGAGTGAGTTGCCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGC56970.6758039184053067No Hit
TTAAGATTCGAGAGGGCAGAATAATAAAAGAAATATTTCCAACAGGAAGA49860.5914618811951657No Hit
TTGAGTGAGTTGCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCA45630.5412837071587528No Hit
TCAAGTGCCAATAGATGCTCATCTTCACTGTTTATTATTCCTTCGTTGGA41010.48647917665089757No Hit
TTGAGTGAGTTGCAAAAGATAAATCGCAATTATTCTTCATGTCTTGCTTC40470.4800734523058235No Hit
TCGAGCTGGATGAGAAAGTGGATGATCTCAGAGCTGACACTATAAGTTCA36640.43464025926576166No Hit
TTGAGTGAGTTGCTTCGGAGCTGCCTATGAAGACCTGAGAGTTTTGTCTG35770.42431992559869797No Hit
TCAAGAATCATGTTCCTCTGATCAGTATTTAGTATAGATCTGTTCCTTTT33800.4009508941916688No Hit
TTAAGGAATCTGCATTCACTAACCCCTGAAGCTGGGCCATCAGTTATCAT30890.3664311574432145No Hit
TAGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA28750.341045509112736No Hit
GCCGCCTTACACTGACTTGAGTAAATTTCCTTTGACATCTGCATCACGTC25790.30593264973973777No Hit
TTGAGTGAGTTGCTGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATC24740.2934770746243161No Hit
TCAAGTCTTTGCTATTCTTACTGTATCTCTGATCAAATACACAATCATGA24570.2914604577008669No Hit
TAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCCG23540.2792421316352628No Hit
TCAAGAACTGATAAGGCTCCCCATTGCTCCTTAGCTTTGGTGGTGAGAAA20860.24745075895971036No Hit
TCAAGAGAAACTGTTTTACCAGAGTCTGACCAATTGGAGTTTTATTGGAG20540.24365477416262948No Hit
TGAGAAAATACCATGCACAAGATTATTAAATCTTTCATGTCCTCCTTCTT20530.24353614963772072No Hit
TTGAGTTTTGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCAT19810.2349951838442887No Hit
TTGAGGCTTGTATTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAA19750.23428343669483603No Hit
TTGAGTGAGTTGCATTGACAGGCACAGAATTCAAGCCTAGATCAGCATTA19720.2339275631201097No Hit
GCCGCCTTACACTGACTTGAGTAATAGAGCAGTATAGTATGGTTATCCAA16800.1992892018467466No Hit
TCCAGTTTTGGGCTCCAATGACCAGATCTGGGGGGAACGAAGTAGGTGGA15520.18410526265842306No Hit
TTGAGTATTTTGAAAGTGAAATTTATTTTATTACAAATGTTTTTCTTTTA15250.18090240048588604No Hit
TCAAGGGGAGGTCAATGTGACTGGTGTGATACCACTAACAACAACACCAA14900.17675054211407884No Hit
TCAAGCTCACTCTCCGCCACCCCTAAAGACCTCTTCCTCCCTCCTCTGAA14450.17141243849318383No Hit
TTGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG14430.1711751894433663No Hit
TTGAGTGAGTTGCTGGAGCAAAGTAATAAAAGGGGCCTTGCCTTTAATTG13780.16346459532429575No Hit
TTGAGTGAGTTGCAGGAGCAAAGTAATAAAAGGGGCCTTGCCTTTAATTG13610.1614479784008465No Hit
TTGAGTGAGTTGCCTAGATCAGCATTAAAATGCAAGGGTTTCCATGTTCC13200.15658437287958663No Hit
TTGAGTCAGATGCATTCATTAGTTCATTTATCCCATGTAGCTCACTTGCT13160.1561098747799515No Hit
TTAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAACTCCAGATCCATCCT12810.1519580164081443No Hit
TCAAGATTCTCAGGTGGTCAGCTGGTTCTTCTACTGGTGTTTTCTATCCC12380.14685716183706685No Hit
GCCGCCTTACACTGACTTGAGTTTTGTCTGCATTAACAGGCACAGAATTC12380.14685716183706685No Hit
TTGAGTGAGTTGCATTAGCAGGCACAGAATTCAAGCCTAGATCAGCATTA12330.14626403921252296No Hit
TTGAGTGAGTTGCATGCATTAACAGGCACAGAATTCAAGCCTAGATCAGC12300.14590816563779663No Hit
TTGAGTAGAAGTAACGATCTCTTTGATCAATGGGGGTATGAGGAATCACC12100.14353567513962107No Hit
TTGAGTGAGTTGCGTTAACAGGCACAGAATTCAAGCCTAGATCAGCATTA11970.14199355631580696No Hit
TTGAGTGAGTTGCAAGGGTTTCCATGTTCCAGCAAAGGAACAGGTAGAAG11790.13985831486744896No Hit
TTGAGTGAGTTGCATTAGGATTAAAGATCAGACAAAGACAAAGATTTGGA11690.13867206961836118No Hit
TTGAGTGAGTTGCATTTACAGGCACAGAATTCAAGCCTAGATCAGCATTA11320.13428296219673638No Hit
TTGAGTGAGTTGCAGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATC11020.13072422644947307No Hit
GCCGCCTTACACTGACTTGAGTCATTCATCATCTTGAGTAAATTTCCTTT10560.1252674983036693No Hit
TAAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC10460.12408125305458152No Hit
TTGAGTGAGTTGCTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAG10420.12360675495494641No Hit
GCCGCCTTACACTGACTTGAGTATTTCGTTGTGGAGTTCATCCATGGCAC10250.1215901380314972No Hit
TTGAGTGGCAGGAGCAAAGTAATAAAAGGGGCCTTGCCTTTAATTGGTGA10230.12135288898167963No Hit
TTGAGTGAGTTGCATTGGGATTAAAGATCAGACAAAGACAAAGATTTGGA10040.11909902300841285No Hit
TCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGTCTCTTAATTCCT9390.11138842888934229No Hit
TTGAGTGAGTTGCGAGTGGCAGGAGCAAAGTAATAAAAGGGGCCTTGCCT9280.11008355911534574No Hit
TTGAGTGAGTTGCTTCGGGGCTGCCTATGAAGACCTGAGAGTTTTGTCTG9150.10854144029153164No Hit
TTGAGTGAGTTGCTTTAACAGGCACAGAATTCAAGCCTAGATCAGCATTA9080.1077110686171702No Hit
TTGAGTGAGTTGCATTACCAGGCACAGAATTCAAGCCTAGATCAGCATTA8800.1043895819197244No Hit
TCAAGAGCATGGAATAGAGACTCCTAAGTATCTGGCTGATTTGTTTGATT8600.10201709142154886No Hit
TGAGTGAGTTGCGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAG8500.10083084617246109No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTT100.007237737144.007729
GTCGCGT100.007237737144.007729
TCGCTAC100.007237737144.007729
ACGTGAA800.0144.007727
AGTTCGC100.007237737144.007728
GTTGTCG151.264045E-4144.00779
GCCGCCT50600.0142.635471
CCGCCTT50850.0142.016892
CGCCTTA51150.0141.043183
CCTTACA51500.0139.944825
TTAAGGT107350.0139.160841
CTTACAC52000.0138.74596
TCAAGTG20200.0137.640521
ACACTGA52400.0136.99979
ATTCGAG5700.0136.428366
TAGAGTG7400.0136.2721
GATTCGA5750.0135.234015
TTAAGAG20000.0135.05531
ACACTAG14300.0134.944296
TAAGGTC110800.0134.901752
Produced by FastQC (version 0.11.2)