FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_361_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_361_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2699485
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA46200.17114375519775069No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC45530.16866180030635472No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT40610.15043610170087998No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT40190.14888024938090044No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT28090.10405688492434667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA2500.0100.801662
AGCACGC7850.086.227561
GCACGCA8150.083.044312
CGCACTT8650.079.074985
GAAACAC25500.078.493964
AAACACG26100.076.137775
ACGCACT9750.073.8464
CACGCAC9400.072.767153
AACACGA19750.071.089786
GTAACAA42350.065.794434
GCGGTGC9650.064.1739961
GAAGCGG6700.063.411081
ACACGAG22700.062.1684237
CGAGCAC5950.060.50523
TAACAAG49000.056.718255
GGTGCGT2850.055.579863
AACACGT11200.053.358026
ACGTGCA12300.050.9276662
TAGTAAC56900.049.7301672
TAGAAAC51850.049.019132
Produced by FastQC (version 0.11.2)