FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_346_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_346_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796052
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAGATCCTATCTGAAGTTGAAGTGTATGGGTCACACGGCCGGCTTCAA252463.171400863260189No Hit
TCAAGAACCCTGCAGAAATGGACAGCAGGCATCTCCAAAGTGACCTGTGT250283.144015717566189No Hit
TCAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCT166612.0929537266409732No Hit
TTGAGTCTTTGAGAACAGTTTTTTACTCCTTTATACCATTTTTTGTTTAG150121.885806454854708No Hit
TCAAGCTCACTCTCCGCCACCCCTAAAGACCTCTTCCTCCCTCCTCTGAA148911.8706064427951943No Hit
TTGAGTGGCTCTGGGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCA133871.6816740614934704No Hit
TTAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAACTCCAGATCCATCCT96331.2100968278454172No Hit
TTAAGTCTCTGTGATAACCACGAAGGCTGTGTGAAAAAGGTAAGAGGATT94951.192761276901509No Hit
TCAAGCAAGTGAGTTTTAGGAAAACACTTTTGTTAGGAAGATCATGTGGG83501.0489264520408215No Hit
TCAAGTATAACTGAGAGGTCACATTGGAGGCTGTGGGCGTGCCGCCTGAC70060.8800932602392808No Hit
TTAAGAGTTAGTTACTGACAGAGTGCCGCAGCAGTCTGAACACGTTGATG64930.8156502339043179No Hit
TCAAGTCTTCACAAGCGCTGATCATTTTTACCACCTGTAAGATACCTCTT64860.8147708943636848No Hit
TCAAGGCATTTTGCTTTTTGACTTTCTGGAGGGTGAAAGAATGATAACAT62200.7813559918196298No Hit
TCAAGATTCTCAGGTGGCCAGCTGGTTCTTCTACTGGTGTTTTCTATCCC58180.730856778200419No Hit
TTAAGTCTTTGTAGTTGGTGTGCCCCGTGCCTGAAATACACTCCCCATTT50560.6351343882057956No Hit
TCGAGCATAGAGAGCAAGGGCAGAGTAGTAGGAGTCAGAGAGACTGAGCA43730.5493359730268877No Hit
TTGAGTTTGCCTCTGTCAACAGATGGAATGGAGCCTGGGGCTGGGTGTCA35910.45110118434474133No Hit
GCCGCCTTACACTGACTTGAGTCTTTGAGAACAGTTTTTTACTCCTTTAT34600.4346449729414661No Hit
TTGAGTATCCTTCAGAAGCTGAAGCCCTTTATCCCAGAGCTAAACACACA34590.43451935300709No Hit
TTAAGTTTTAGGAGTGTCAGACTCCTTCCTGGATCTGAATGCCCACCTAC32840.4125358644912644No Hit
TTAAGATGGAATGTTAAATACACTCTTTTATATTGGAAATAAAATGAAAA32770.41165652495063143No Hit
TTAAGTTGAGAAATAAAGTTCACAGTTTCAAAAGGAAGGTATGCCATAAC31350.39381849426921856No Hit
TTGAGTTTCATCTGGTTTACCTTTCTGTTTTAACAGGACAGCATTGAGTT27700.34796721822192517No Hit
TGGAGATGTTGAGTTTATCTGAATTTTTCTCGTGTCTTCCTGCTCCTCCT26560.33364654570304453No Hit
TTAAGAAAGCACTTAGGCCTCTCCGAGACCGTTTTTCTCTTCTGTAAAAT25370.3186977735122831No Hit
TTGAGTATGCTTCTTAAAATTCAGTAGGTAGTGGCGATTTTGCATGTGAA23520.29545808565269605No Hit
TTGAGTTTGCCATTTGTATTTACTTTGTTTCAACTTAAAAAAATAAAAAC21460.2695803791712099No Hit
TCGAGCATACAAAATAGGGAAAAGCACAAATCTGATAAGAGAAATACCCT21140.26556054127117323No Hit
TTAAGGCATTTTGCCTGTTGACTTCCTGGAAGGTCAAAGAATGGTAATAT20660.2595307844211182No Hit
TTAAGCTCTATCAACTTCAAGACACTACTGTAAGTAAAATGATATCAGCC20320.2552597066523292No Hit
TTAAGCAAATGGCCCTGAGGTGGAGACATGTGAGCAGAGGAGAGAGATGG20290.2548828468492008No Hit
TCAAGGGCCTTTACTTTTGCAATTTTCCCTGCTTGGAGCAATCTTCCTCC19550.24558697170536598No Hit
TCCAGCCTGGGGGAAACGTGAGAGGGTGGTATGAAGATACACACGATGCA19400.2437026726897238No Hit
TTGAGTATACTTCAGGGATCCAGTGGTTCCGGAATTTAATTCTATAAAGT18710.23503489721776968No Hit
TCGAGCTGTTGAGGCGTGTCAGGAAATCTAGCAACATCTGTATCCTCCCC18200.22862828056458623No Hit
TCAAGTGGTAAAAGATTATATACAATAAATATCTCCCATACCCTAGAATT17550.2204629848301367No Hit
TCGAGATTGGGAATAGGTAATAAAGAAAATGAATCCTGAGGGCTGATAAA17550.2204629848301367No Hit
TTAAGCATCCCAAAAAACTGTTGCCATGACCTTTGCTCTTGGCCAGGTCA17250.21669438679885233No Hit
GCCGCCTTACACTGACTTGTGACGACACAATGTAATGATGGCATTGAGTC16670.20940843060503586No Hit
TTAAGAGTACAGGTTGAGACCATCCTGGCTAACACGGTGAAACCTCATCT16660.20928281067065974No Hit
TGAGTGGCTCTGGGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCAA16480.2070216518518891No Hit
TTCAGGAGGGTAATGAGAGTTGCAGCAAGTATAAATCTTGTCACAACCCC16260.20425801329561388No Hit
TCAAGTAGCTGGGACTATAGGTGCTGGCCACCATGCCCATGTAATTTTTT15480.19445965841427443No Hit
TTGAGTTTCATGGTGCCCCAGATAGATTATACTCAGAGACTTACTCATAG15300.1921984995955038No Hit
TTGAGTTGTGTCCAGGTTTTGCGTGGCCATGGAAAATCAGGTGAAGGACA15220.19119354012049464No Hit
TCAAGACTTTCTAGAGGGCCCAAAACAAAAAAACCCCAATAACACATGCT14720.18491254340168733No Hit
TTGAGTATGCTTCCGGGTTATTAGTAGAAACACGAGCCAAAATAAAACTC14600.18340510418917358No Hit
TCAAGAAGAAGCAAGTTATTTGCTTTCAGAACACACTGAAGGGACAGGCA14070.17674724766723782No Hit
TTGAGTCTTTGTAGTTGGTGTGCCCCGTGCCTGAAATACACTCCCCATTT13820.17360674930783415No Hit
TTGAGTTTGCGGATTCCAAAAGTGAGACAGTGTAAGTACCGCCAGCCTGG13630.17121997055468738No Hit
TTGAGTTTATCTGAATTTTTCTCGTGTCTTCCTGCTCCTCCTTCCTTGTA13610.1709687306859351No Hit
TCAAGGCTCTTTGTTGCGGCCGGGCGCCGTGGCTCACGCCTATAATCCCA13400.16833071206403602No Hit
TTGAGTATGCTTCACAGACTGGAGGGAAAAAGGAAACAGCTGAATACAGC12080.1517488807263847No Hit
TTGAGTATGCTTCTTTCACAGACTGGAGGGAAAAAGGAAACAGCTGAATA11760.14772904282634802No Hit
TTCAGGGATCCAGTGGTTCCGGAATTTAATTCTATAAAGTGAAATTAATA11450.1438348248606875No Hit
TCAAGTTTCAAACACAAAAATAAGATCCACGATAGCACCAAACTTCTCTA11320.1422017657137976No Hit
TTGAGCAAGTGAAGGGAAAAACTTTGCTGCCTCTTCCAGCAGGCTCAGAA10600.13315713043871505No Hit
TTGAGTATGCTTCTTGAAATTCAGTAGGTAGTGGCGATTTTGCATGTGAA10540.13240341083245818No Hit
TTGAGTATGCTTCTTAAACTTCAGTAGGTAGTGGCGATTTTGCATGTGAA10510.13202655102932973No Hit
TGGAGGGAAAAAGGAAACAGCTGAATACAGCAAGAAACACACGCTTCTTA10480.1316496912262013No Hit
TTGAGTATGCTTCAGAAGGAGAGGGCCCGTGATGAAACACTTTCTCCTGC10230.12850919286679766No Hit
TCAAGAAGAAGTTTAAAATATATTTCTTTTCTTTATATGTTTATTTTTTA10150.12750423339178849No Hit
TTGAGTACTCTTCTTATGGGCAAAAGGTGAGATGGATACCCTTAGCAATC10070.12649927391677931No Hit
TTGAGTATGCTTCGAAGGAGAGGGCCCGTGATGAAACACTTTCTCCTGCT9920.12461497490113711No Hit
TTGAGTATGAATCCTGAGGGCTGATAAATCAACAAAGTGAGTCCAATAGC9710.12197695627923803No Hit
TGAGTCTTTGAGAACAGTTTTTTACTCCTTTATACCATTTTTTGTTTAGA9630.12097199680422886No Hit
CAAGAACCCTGCAGAAATGGACAGCAGGCATCTCCAAAGTGACCTGTGTA9550.11996703732921969No Hit
TTGAGTAAGGGTGCAGGGAGCAGCCCTGTGCATTGCCTAGGGAGGGGTTA9280.11657529910106375No Hit
TTGAGTCCAATAGCTGCCCCAGCTTACCACATTAATTAGTCTTCAGCCGA9200.11557033962605458No Hit
TCAAGCAAAAAAAGGACCGTGTCCCGTCTCGTTGCTTCTCACCTTACTGA9130.11469100008542155No Hit
TTGAGTAAGTTAAGGGTTTGGGATGGGGGCGTTATCCTGGGTTGTCCACC8900.11180174159477019No Hit
TTGAGTAAGCCTGGGTGCACCTTCAGAGGAAGAAATAACAGAGGCTGCAG8720.10954058277599955No Hit
TCAAGAAACTACTGATGGGTGTGTTCTGCCTTAACAGGGATATAAAGAAA8210.1031339661228161No Hit
TTGAGTATGCTTCCGGGTTATTAGTAGAAACAACAGCATTTTGTGTCTAA8040.10099842723842162No Hit
CAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCTG8000.10049594750091702No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCAA151.2640315E-4144.007357
CGCCTTA53850.0140.923133
CCGCCTT54350.0140.289062
TCGAGCA8350.0138.842151
ATCCTAT30350.0136.178286
TTACACT55750.0135.999777
TTAAGGC13950.0135.24121
CTTACAC56500.0134.321886
CGAGCAT8550.0133.051072
GCCTTAC57350.0132.69944
TAGTTAC10250.0130.660329
TACACTG58200.0130.39848
ACACTGA58300.0129.927739
CTATCTG32350.0129.76249
GATCCTA31950.0129.575935
CCTTACA59050.0128.879095
TAGAGTA7700.0128.118481
AGAACCC33000.0126.543964
AAGATCC32650.0126.356863
TAAGATC32700.0126.163662
Produced by FastQC (version 0.11.2)