FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_338_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_338_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687538
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC510237.4211170873464445No Hit
TTGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA187092.7211586850472265No Hit
TTAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAACTCCAGATCCATCCT179982.6177462191180707No Hit
TCAAGAATCATGTTCCTCTGATCAGTATTTAGTATAGATCTGTTCCTTTT154932.2534027210132384No Hit
TTAAGAGCACACAAGAAGCTATAAACAAGATAACAAAAAATCTCAACTCT142272.0692674441267247No Hit
TCAAGATGATGAATGACTCAATGGCTAAGAAAACCAGTGGAAATGCTTTC111081.6156197911969956No Hit
TTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGGA93241.3561432240836142No Hit
TCAAGCCGTCCAAATCTTTGTCTTTGTCTGATCTTTAGTCCTAATGCAGC89661.3040733748534628No Hit
TCGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG61640.896532264398477No Hit
TTGAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAA57870.8416989315499652No Hit
TCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCT57290.8332630341886558No Hit
TTGAGTACTGAATGGGAGATGATGCAAAAGATAAATCGCAATTATTCTTC53600.7795932733899799No Hit
TCAAGAAGGAAAACTAGTAAAAGGATCCTTTGGAAAAGCACTAAGAGTAA49270.7166149361926177No Hit
TTGAGGAACAATGCTACGCTAAGTGTTGCAACCTTTTTGAGGCCTGTTTT47650.6930526021834429No Hit
TCAAGTGCCAATAGATGCTCATCTTCACTGTTTATTATTCCTTCGTTGGA46670.6787988445729545No Hit
TCAAGTAGCTGGGACTATAGGTGCTGGCCACCATGCCCATGTAATTTTTT41690.6063664844706765No Hit
TTAAGATTCGAGAAGGCAGAATAATAAAGGAAATATTTCCAACAGGGAGA39860.5797497738306828No Hit
TTAAGAGTACAGGTTGAGACCATCCTGGCTAACACGGTGAAACCTCATCT38480.5596781559710154No Hit
TGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGAC35240.5125534879526659No Hit
TCGAGCTGGATGAGAAAGTGGATGATCTCAGAGCTGACACTATAAGTTCA34790.5060083951723395No Hit
TTGAGTAGATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA31340.45582935052317114No Hit
TTGAGTTGATTGGTTGTTCTGCTTTTAGCTCTTTGAGGAATTGCCATACT30700.4465207741244847No Hit
TCAAGAATCATGTTCCTAGTGCCTCCATGGCATTCTGTGAGGCTGCTTGA30290.44055746736907636No Hit
TTAAGAAATCTGCATTCACTAACACCTGAAGCTGAGCCATCAGTTATCAT29550.42979442590809525No Hit
TTGAGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCTTGC28940.4209221890280974No Hit
TTAAGATTCGAGAGGGCCGAATAATAAAAGAAATATTTCCAACAGGAAGA27820.4046321803303963No Hit
TCAAGGGGAGGTCAATGTGACTGGTGTGATACCACTAACAACAACACCAA27730.40332316177433103No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTGTAGAATTCGCCCAGTTTCAC26620.3871785995828594No Hit
GCCGCCTTACACTGACTTGAGTAAATTTCCTTTGACATCTGCATCACGTC21470.3122736488746804No Hit
TTAAGGAATCTGCATTCACTAACCCCTGAAGCTGGGCCATCAGTTATCAT21190.3082011467002551No Hit
TCAAGACACTAGGATGTTCCAAGGGGTGAGAGGAGAAATAAATTTTCTTA20970.30500132356320664No Hit
TCAAGAATCATGTTCCTCTGATCAGTGTTTAGTATAGATCTGTTTCTTTT20490.2980198912641919No Hit
TTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTATCC19440.28274800811009715No Hit
TTGAGCTAAAAAGAATATCAGGAAGAGGATTCAAAAATGATGAAGAAATA18910.27503934327993507No Hit
TTGAGTTTATCTGAATTTTTCTCGTGTCTTCCTGCTCCTCCTTCCTTGTA18510.2692214830307561No Hit
TTGAGTACTGAATTCGAGCCTAGATCAGCATTAAAATGCAAGGGTTTCCA18100.2632581762753477No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTATAGAATTCGCCCAGTTTCAC14420.2097338619829013No Hit
TTGAGTGTGGATGCTTAAAAAACACTTCCTCTAGCCGAATGGTATGGTGT14300.20798850390814763No Hit
TTGAGTACTGAATGGGAGCAGCTCTGATGTCCATCAAGCTCCAGTTTTGG13650.19853448100323182No Hit
TTGAGTACTGAATATCCCAGAAATAGTGTTGAAGTACAATCTAATGGACC12800.18617152797372655No Hit
TCCAGTTTTGGGCTCCAATGACCAGATCTGGGGGGAACGAAGTAGGTGGA12760.18558974194880865No Hit
TAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCCG11890.17293589590684444No Hit
TTGAGTTCCAATACTGAAAGTTTTGGGGCTATTTACTCTGTCTCCATTGA11670.169736072769796No Hit
TTGAGTCTCCATAGAGGTTCTTCATTTGGGTTTTGTTATCTGAATGGAAC11300.16435455203930546No Hit
TTGAGTACTGAATTGCCCAATATGGGTGAAAACACCTTTGAAGCTTGCCA11110.16159106842094545No Hit
TTGAGTACTGAATCCAGGGCTGCTGGAACAGCTTGTAGTATGACTGGCCC10780.15679133371537282No Hit
TCAAGAATCATGTTCCTCTGATCAGTGTTTAGTAAGGCACTCGGCTCATT10700.15562776166553702No Hit
TTGAGTGCTGAAGACAGGTCAAGTATAAAGAGGGCTGAGATTGGACCACT10570.15373695708455387No Hit
TTGAGTACTATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAA10240.14893722237898122No Hit
TTGAGTACTGAATGGGGGATGATGCAAAGGATAAATCGCAATTATTCTTC10240.14893722237898122No Hit
GCCGCCTTACACTGACTTGAGTAATAGAGCAGTATAGTATGGTTATCCAA10120.14719186430422754No Hit
TCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGTCTCTTAATTCCT10050.14617373876062123No Hit
TTGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG10040.14602829225439176No Hit
TTAAGTGTTGCATTGAAGAGATTTTATTTTCCTCTATAGGTGGTCAGGGG9860.14341025514226122No Hit
GCCGCCTTACACTGACTTGAGTCATTCATCATCTTGAGTAAATTTCCTTT9700.14108311104258964No Hit
TGAGTACTGAATGGGAGATGATGCAAAAGATAAATCGCAATTATTCTTCA8460.12304774427013489No Hit
TTGAGAAAATACCATGCATAAGATTATTAAATCTCTCATATCCTCCTTTT8270.12028426065177487No Hit
TCAAGAGCATGGAATAGAGACTCCTAAGTATCTGGCTGATTTGTTTGATT8110.1179571165521033No Hit
TTAAGTCACACAGAGGCCCCTGACCACCTATAGAGGAAAATAAAATCTCT7780.11315738184653067No Hit
TGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGGAA7760.11286648883407172No Hit
TCAAGAGCATGGAATAGAGACTCCCAAGTATCTGGCTGATTTGTTTGATT7700.11199380979669488No Hit
TGAAGCCATCACTCCCCATGGTGGTTTTGTACATTGTTTTCACCTCTTCT7500.1090848796721054No Hit
TCAAGACCCTTCACTCTAGCAAGAGAGTCTACTGAAAGGCAGCAGCACAT7160.10413969846030327No Hit
TTGAGTCCCCATAGAGCTTTGCCATTTGAGTCTCGTTGTCAGAGTGGAAC7130.10370335894161486No Hit
TTGAGTACTGAATTTGCCCAATATGGGTGAAAACACCTTTGAAGCTTGCC7110.10341246592915593No Hit
TAGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA7070.10283067990423801No Hit
GCCGCCTTACACTGACTTGAGTGTGGATGCTTAAAAAACACTTCCTCTAG6930.1007944288170254No Hit
TTGAGTACTGAATGCAAGCCTAGATCAGCATTAAAATGCAAGGGTTTCCA6930.1007944288170254No Hit
GCCGCCTTACACTGACTTGAGTATTTCGTTGTGGAGTTCATCCATGGCAC6890.1002126427921075No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCCG100.007238651143.999732
CAAGCGG100.007238651143.999732
GTCCACG100.007238651143.999737
GCCGTAC151.2642243E-4143.99974
GCCGCCT60850.0143.568191
CCGCCTT61550.0142.245062
CGCCTTA61500.0142.126543
CCTTACA61900.0141.208115
CTTACAC62350.0140.304466
TTAAGGC21600.0138.37331
TTAAGGT58600.0138.01941
CCGTCCA10400.0136.384346
TCAAGGC9900.0135.311811
TAAGGTC60050.0134.767432
AAGGTCT59800.0133.40443
CAAGCCG10750.0133.283452
AGCCGTC10700.0133.233374
AAGCCGT10700.0133.233373
CGAGAAG5150.0131.41729
TACACTG66850.0131.29078
Produced by FastQC (version 0.11.2)