FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_338_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_338_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3547032
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA88430.2493070262687227No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA57520.16216374704259787No Hit
TAACCGTTACACAGCAAAAAGACCCTTTGTCAAATTAAATGTGGAGACTG48860.13774896871525263No Hit
TTCTCAGTATGGAAACAGGTGTTGCCATATTGTAAAGAGCCATGGCTTCG47750.13461959181648206No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG42830.12074884015706652No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGCA100.0072396104144.001722
GTAACAA23950.098.003974
AGCACGC5750.090.1741261
AAACACG26100.085.2411965
CGCACTT5500.079.854375
GCACGCA6600.079.6373142
AACACGA19800.078.1827556
TAACCGT16450.076.61071
CCGTTAC17000.074.117484
ACCGTTA17050.073.900143
CGTTACA17150.073.469225
GTTACAC18050.069.806926
CGCACTG2400.068.999855
AACACGT11100.066.811616
ACGCACT9050.065.237434
ACACGAG23850.064.906437
TAGTAAC37550.064.6186142
GAAGCGG12050.063.9460951
GAGCGCC350.00366622561.7150237
TAACAAG40400.057.920675
Produced by FastQC (version 0.11.2)