FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_337_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_337_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733152
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGTACAGAATATAGATACAGCTTAAATTTTGTCTTACAAAGTCAGCA247633.3776079175941685No Hit
TTAAGATCCTATCTGAAGTTGAAGTGTATGGGTCACACGGCCGGCTTCAA212692.9010355287853No Hit
TCAAGGCCCATGGTCCGTGCTCGCTTCCAGCTCCAGCTTCCCGCTTGGGA194352.6508827637379424No Hit
TCAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCT146511.9983577757409103No Hit
TTGAGTCTCTGCTTGTGATTCTTTGGCTTTGTATCTAGGAGTGGAAGCGC117171.5981679106106237No Hit
TCGAGCATACAAAATAGGGAAAAGCACAAATCTGATAAGAGAAATACCCT111511.5209670027497708No Hit
TTGAGGTCTGGAAATTTAGTTTCTAACAAGTTTGTTTGTTGAAAAGGGCA93401.273951377067784No Hit
TTGAGTTGAAGAAATTGGTGGCAAATTCTGCCTCCTATAATATTACACTT88321.204661516302213No Hit
TCGAGCTGTTGAGGCGTGTCAGGAAATCTAGCAACATCTGTATCCTCCCC61420.8377526079175942No Hit
TCAAGTGTGGCCCTAGCACCAAGGAAGCTTCATTAGTGATTTACCTACTA58740.8011981144428441No Hit
TCAAGGGGGTACAAGGGGAAGCATCTTTAAGATGAAGGAAAGTCAGGAAC46740.637521277988739No Hit
TTGAGTTAGGGAGTTACTGATCTCTCTGGTAACTGAACAGGTTTAGAAGT45580.6216991837981756No Hit
TTAAGAAGACAGAAGGGCAAAGCCACAGGTGGTAAAGTCATGATTGTTGA44730.6101054078826764No Hit
TTAAGCCAAAGCCCACGGCAATTCTGGCTGCTTTCTCCTGAGGACCACAT43960.5996028108768714No Hit
TCAAGCCAGTCTCTAATATCGTTAATGCATTTGATAAGATAAAATCATTA43160.588691021779931No Hit
TCAAGGCATTTTGCTTTTTGACTTTCTGGAGGGTGAAAGAATGATAACAT41110.5607295622190214No Hit
TTAAGATTATCTCAGGCCCTGAACTTTTCAATTCACAAGTGAAATTCTAT40660.5545916808519925No Hit
TTAAGTGGTTTGAAGAACAGTAGGGAAGAGAACTATGGCTTGCCTTCCCT35840.488848151542927No Hit
TTGAGTTTCTGGGATTACAGCCATAAGCCTGGCCTGGTAAATTTCAAGCA31490.4295152983283139No Hit
TTAAGTTCCATCAAGTTCAAGACACTTTTATAAGTGGTACTACCAGCCAC25620.3494500458295142No Hit
TCAAGGCTCTTTGTTGCGGCCGGGCGCCGTGGCTCACGCCTATAATCCCA24840.3388110514599974No Hit
TCAAGGGTGTAGACTTTATTTGGTAAGCAAAGGGCTGCACAGATGGATTT23790.3244893282702632No Hit
TTGAGTTCAAATGTGGCAGTGTTCCAGAAGTAGTACTGTGCTGCATATCA19120.260791759416874No Hit
TCAAGTGAGGAAAATGGAACTTGAAGAACTTTATATTCTTTGTCTGTATG18700.2550630701409803No Hit
TTGAGCCTTTGTGTCCAAAGCAAGAACTACTTCTGGATTTTTAGTCAAAT18400.2509711492296277No Hit
TCAAGAGGCAGGGCAGTTGTATTCCGAACCCCAGTGGATACTTACTGAGC17750.24210532058836368No Hit
TTGAGTGAAGAAGGCACAGGTCTTAAAATAGGTTTTCTTGCAGGGAGAAC16870.2301023525817293No Hit
TTGAGTCCTGTTTGGAGGACAGCTGCTCCCACATCTGGGGGCCTCTGGAT16550.22573763694295318No Hit
TCAAGTTTGCCAGGATATGATAAAAGGATATATTTATATGTGTGTGTATA15940.21741739775653618No Hit
TTGAGTCCAGTCCTGGTTCTATCTCTTAATACCTGTACCGTTTTGTACAA15870.21646261621055388No Hit
TTGAGTTCGGAAGAAATGAATATTCCAAATTTCCAATTTTTACAATATCT15600.21277988739033651No Hit
TGAGTCTCTGCTTGTGATTCTTTGGCTTTGTATCTAGGAGTGGAAGCGCT15560.2122342979354895No Hit
TTAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAATTCCAGATCCATCCT15160.20677840338701936No Hit
GCCGCCTTACACTGACTTGAGTTAGGGAGTTACTGATCTCTCTGGTAACT14670.20009493256514338No Hit
TTGAGTTTGGAAGAGTTGGAGAACTTCTGAATTTAAGCAGGCAAAAATGA14640.1996857404740081No Hit
GCCGCCTTACACTGACTTGAGTTTGGAAGAGTTGGAGAACTTCTGAATTT13720.18713718301252674No Hit
GCCGCCTTACACTGACTTGAGTTGAAGAAATTGGTGGCAAATTCTGCCTC13650.18618240146654447No Hit
GCCGCCTTACACTGACTTGAGTTGGAGAACTTCTGAATTTAAGCAGGCAA11680.15931212081532886No Hit
TTGAGTTCGGAAGGGAAATGCAAATATAAAACACAGTGAGATACCACCTC11290.15399262363057048No Hit
CAAGTACAGAATATAGATACAGCTTAAATTTTGTCTTACAAAGTCAGCAC11220.15303784208458818No Hit
TCAAGCAAGTGAGTTTTAGGAAAACACTTTTGTTAGGAAGATCATGTGGG11000.1500371000829296No Hit
TTGAGTCTTTGTGTCCAAAGCAAGAACTACTTCTGGATTTTTAGTCAAAT10880.14840033171838854No Hit
TTGAGTTGCTGTCTCTTTCCCTCCCTTCCCCTGGCAGACAGAACCCTTCA10860.14812753699096504No Hit
TTGAGTATCCCAAAATGTTGCCATGATCTTTGCTCTTGACTGGTCCGCAT10850.14799113962725327No Hit
TTAAGTTAAAAAAATAAAGTTCACATTTTTTATGTTCATTATGTAATTCA10380.14158046353280085No Hit
TTGAGTAAGAAAGCATTCCGGTTCCAACTTGGTCAACGAGTCACTTTTAG10370.14144406616908908No Hit
TTAAGAAAGCACTTAGGCCTCTCCGAGACCGTTTTTCTCTTCTGTAAAAT10310.14062568198681855No Hit
TTGAGACCAACTCACAGAGGCTGGGACTCTGATTCAAGCTTAGGTTGCTG10180.13885251625856573No Hit
TTGAGTTGGAGAACTTCTGAATTTAAGCAGGCAAAAATGAACTTATTTAT9950.1357153768931954No Hit
TTAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAACTCCAGATCCATCCT9540.1301230849810135No Hit
CAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCTG9370.12780432979791365No Hit
TTAAGAATCTCATTTTTCAACTATAAAATTGATATATGGAGGTTAAACTT9050.12343961415913754No Hit
TTGAGTTTTGATGGGAAATCATTGGGCATCCACCTTTCAGTCCTGATTTG9040.12330321679542577No Hit
TTGAGTGCGACTGAATAGTAGATCCTGGGGAAAAGTATAGTAGAACCAGA8720.11893850115664965No Hit
TCAAGTCTTCACAAGCGCTGATCATTTTTACCACCTGTAAGATACCTCTT8570.11689254070097334No Hit
TTGAGCCAGAAAAGCAAAGCCCTCTAGAACCCAGCTGCTCCAAGTGTGGT8560.11675614333726157No Hit
TTGAGTTCGGAAGATTCCAAATTTCCAATTTTTACAATATCTAGTTAAAT8520.11621055388241455No Hit
TTGAGTGGTTCTCTATGTGTACATTGTCTTTGGAATGATTGAGAGGTGGA8430.11498297760900877No Hit
TTAAGCTGCTGGATCTGTGGGGAAAACAAACATCTGAGGTCCAGAATTCG8260.11266422242590896No Hit
TTGAGTTCGGAAGGAGAGGGCCCGTGATGAAACACTTTCTCCTGCTTTCA8240.11239142769848545No Hit
TCAAGGGTTACAATAACAATGCTTCCAGCAACTTCTATTTCAATAGCACA8140.11102745406136791No Hit
TTGAGTCTTTGAGAACAGTTTTTTACTCCTTTATACCATTTTTTGTTTAG7950.10843590415084457No Hit
TTAAGCCCTAACACATAGGTAATTAGATTAAACATAAATGGTTCTGAAAT7690.10488957269433896No Hit
TTGAGTTCGGAAGAGATTAATATCCACAATATATAAAGAACTTCTACAAG7680.10475317533062721No Hit
TTGAGTTTATCTGAATTTTTCTCGTGTCTTCCTGCTCCTCCTTCCTTGTA7660.1044803806032037No Hit
GCCGCCTTACACTGACTTGAGTCTCTGCTTGTGATTCTTTGGCTTTGTAT7640.1042075858757802No Hit
TGAGTTGAAGAAATTGGTGGCAAATTCTGCCTCCTATAATATTACACTTT7560.10311640696608615No Hit
TTAAGGCACAAATTCATACCCGCTCCTCAGACCCAGAGCTTCTGATCCAG7520.10257081751123913No Hit
CAAGGCCCATGGTCCGTGCTCGCTTCCAGCTCCAGCTTCCCGCTTGGGAG7460.1017524333289686No Hit
TTGAGTTCGGAAGGACAAAGAAATGAATATTCCAAATTTCCAATTTTTAC7370.10052485705556284No Hit
TTGAGTACGGTGTGATCAAGGACCAAATCAATTACATAGTCAGTTGAGAC7350.10025206232813932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCCT61650.0141.313871
CGCCTTA61950.0140.513323
TCGAGCA13700.0139.795621
CCGCCTT63000.0138.171432
GCCTTAC63900.0136.225364
CTTACAC64250.0135.37126
CGAGCAT14200.0133.352112
CCTTACA65350.0132.982395
TACACTG66600.0130.378378
GATCCTA28150.0129.165185
GTATAAC18850.0129.103459
ACACTGA67100.0128.548439
TCCTATC28250.0128.453117
TCAAGTG33450.0127.426021
CTATCTG28650.0126.408389
TCAAGTA33250.0126.24361
CAAGTAC31300.0125.597442
TCGAGAG1500.0124.81
CAGAATA31500.0123.28
TCAAGGG13150.0123.193911
Produced by FastQC (version 0.11.2)