FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_337_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_337_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3159994
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC57160.1808864194045938No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA54170.17142437612223316No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT52580.16639272099883734No Hit
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG52250.1653484152185099No Hit
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC49200.1556964981579079No Hit
GTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAG39350.12452555289661942No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG37430.11844959199289617No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC36840.11658249984018956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA109250.0122.268541
TAGTAAC109500.0122.1692662
GTAACAA115950.0117.981324
TAACAAG128900.0106.295845
AGTAACA131100.0104.512083
CGCACTT23350.097.130155
AAGAGCG20850.097.041659
ACAAGAG155000.088.263227
AGCACGC25300.083.964121
AACAAGA165800.082.383586
CAAGAGC165300.082.3278668
ACGCACT28450.081.996094
GCACGCA26550.081.084362
CTCGCTC22700.074.21993
TCGCTCT24650.067.180214
GTCTCGC25900.066.4492951
AAACACG50350.065.493235
CGCTCTG25650.063.999695
GCGGTGC21500.063.9715921
TCTGTCG25450.062.526258
Produced by FastQC (version 0.11.2)