FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_328_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_328_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565776
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC307455.434129408104974No Hit
TTGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA271844.804728373066372No Hit
TCAAGAACTGATAAGGCTCCCCATTGCTCCTTAGCTTTGGTGGTGAGAAA179343.1698057181640786No Hit
TTGAGGCTTGTATTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAA122362.162693362744266No Hit
TCAAGACACTAGGATGTTCCAAGGAGTGAGGGGTGAAATAAATTTTCTTA87711.5502601736376234No Hit
TTGAGTAGAAGTAACGATCTCTTTGATCAATGGGGGTATGAGGAATCACC85041.5030683521393626No Hit
TTAAGAGCACACAAGAAGCTATAAACAAGATAACAAAAAATCTCAACTCT82341.455346285455728No Hit
TTGAGTGAAGTTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCA79471.4046194960549758No Hit
TTAAGTGGTTTGAAGAACAGTAGGGAAGAGAACTATGGCTTGCCTTCCCT76121.3454087836882442No Hit
TTAAGATTCGAGAGGGCAGAATAATAAAAGAAATATTTCCAACAGGAAGA68141.2043635643788355No Hit
TTGAGAAAATACCATGCACAAGATTATTAAATCTTTCATGTCCTCCTTCT52200.9226266225502673No Hit
TCAAGAGCATGGAATAGAGACTCCTAAGTATCTGGCTGATTTGTTTGATT51200.90495178303781No Hit
TCGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG48160.85122027091994No Hit
TCAAGATGATGAATGACTCAATGGCTAAGAAAACCAGTGGAAATGCTTTC45320.8010237267045615No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTGTAGAATTCGCCCAGTTTCAC39750.7025748706201748No Hit
TGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGAC35830.6332894997313424No Hit
TTGAGTTTTGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCAT35700.630991770594723No Hit
TTAAGGAATCTGCATTCACTAACCCCTGAAGCTGGGCCATCAGTTATCAT33290.5883954073697011No Hit
TTGAGTACTTTTCTAGACATTCTTTGGCTAATGTCTTCGGTTTTTTGGAC30480.5387291083396962No Hit
GCCGCCTTACACTGACTTGAACTAAAGAGAATATCAGGAAGAGGATTCAA29550.522291507593111No Hit
TTGAGTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGGGTCATAC29480.521054268827239No Hit
TTGAGTCCAGTCCTGGTTCTATCTCTTAATACCTGTACCGTTTTGTACAA28120.4970164870902972No Hit
TTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGGA26420.46696925991911997No Hit
TTGAGTGAAGTTCTTTGGTTGTCCCCATCTCCCACATCTTTGGTGTATCA24170.42720087101609117No Hit
TCGAGCTGGATGAGAAAGTGGATGATCTCAGAGCTGACACTATAAGTTCA19700.34819433839540737No Hit
TCAAGAACTGATAAGGCTCCCCATTGCTCCTTAGCTTTGGTTGTCCCCAT19320.34147789938067363No Hit
TCAAGTGCCAATAGATGCTCATCTTCACTGTTTATTATTCCTTCGTTGGA19000.3358219507366873No Hit
TTGAGTGAACTTCAAGTACCAGTAAAAGAACTGAAAATCAAAATGTCCAA16180.28597890331155795No Hit
TGAGTAGAAGTAACGATCTCTTTGATCAATGGGGGTATGAGGAATCACCC16130.2850951613359351No Hit
TTGAGTGAAGTTCGATTGCCTTCATGAAAAATACGGTGGATTAAACAAAA15510.2741367608382116No Hit
TTGAGTGAAGTTCGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCA14420.25487118576963325No Hit
TTGAGTCTCCATAGAGGTTCTTCATTTGGGTTTTGTTATCTGAATGGAAC14060.2485082435451486No Hit
TTAAGGCATTTTGCCTGTTGACTTCCTGGAAGGTCAAAGAATGGTAATAT13730.24267554650603773No Hit
TTGAGCTAAGGACCTTTGGACCATCCATGCTATGGTTCTTGGCATCCCCT13120.2318938944034388No Hit
GCCGCCTTACACTGACTTGAGTTTTGTCTGCATTAACAGGCACAGAATTC12860.22729843613019993No Hit
CAAGAACTGATAAGGCTCCCCATTGCTCCTTAGCTTTGGTGGTGAGAAAC12740.22517745538870507No Hit
TCAAGCCCAGAATGCAGGGCCCAGCCCCTGTGTGTACTGCCTGCCCAAAG12360.21846101637397133No Hit
TCCAGTTTTGGGCTCCAATGACCAGATCTGGGGGGAACGAAGTAGGTGGA12330.2179307711885976No Hit
TCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGTCTCTTAATTCCT12320.21775402279347306No Hit
GCCGCCTTACACTGACTTGAGTAGAAGTAACGATCTCTTTGATCAATGGG12080.21351206131048328No Hit
TTGAGTGAAGTTCTGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATC11540.2039676479737564No Hit
TTGAGTGAAGTTCATTGCCTTCATGAAAAATACGGTGGATTAAACAAAAG11420.20184666723226152No Hit
TTAAGCATCCCAAAAAACTGTTGCCATGACCTTTGCTCTTGGCCAGGTCA11410.20166991883713695No Hit
TTGAGTGAAGTTCAAGCCTAGATCAGCATTAAAATGCAAGGGTTTCCATG10740.1898277763637906No Hit
GCCGCCTTACACTGACTTGAGTAAATTTCCTTTGACATCTGCATCACGTC10590.18717655043692202No Hit
GCCGCCTTACACTGACTTGAGTCCAGCTTTGACCATCATCTGGTTGTAGA9720.17179944006108425No Hit
TAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCCG8940.15801306524136763No Hit
TAGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA8740.15447809733887616No Hit
TTGAGTGAAGTTCTGCATCAACAGGCACAGAATTCAAGCCTAGATCAGCA8600.15200361980713215No Hit
TTGAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAA8500.15023613585588644No Hit
TGAGGCTTGTATTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAAA8500.15023613585588644No Hit
TCAAGTCCTACCACTGGACCAGAACATCCAGTTCCACCAGCTTATGGGCT8490.15005938746076183No Hit
TGAGTGAAGTTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCAT8390.14829190350951613No Hit
TTGAGTGTAGTCAGATGCATTCATTAGTTCATTTATCCCATGTAGCTCAC8190.14475693560702468No Hit
TCAAGGGGAGGTCAATGTGACTGGTGTGATACCACTAACAACAACACCAA7720.1364497610361698No Hit
TTGAGTGAAGTTCTGCGTTAACAGGCACAGAATTCAAGCCTAGATCAGCA7040.12443087016769888No Hit
TTGAGTGAAGTTCTTGCCTTCATGAAAAATACGGTGGATTAAACAAAAGC7030.1242541217725743No Hit
GCCGCCTTACACTGACTTGAGTCAAGTTCTTCAATGGTTTTGAAATCTCC6800.12018890868470913No Hit
GCCGCCTTACACTGACTTGAGTCTCCATAGAGGTTCTTCATTTGGGTTTT6600.11665394078221769No Hit
TTGAGTGAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGGGTCATAC6550.11577019880659484No Hit
TCGAGCATAGAGAGCAAGGGCAGAGTAGTAGGAGTCAGAGAGACTGAGCA6450.11400271485534912No Hit
TTGAGTGAAGTTCAGATTGCCTTCATGAAAAATACGGTGGATTAAACAAA6420.1134724696699754No Hit
TTGAGTGAAGTTCTGCACCAGTTCTTTGGTTGTCCCCATCTCCCACATCT6390.11294222448460169No Hit
TTGAGTGAAGTTCGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCAT6160.10887701139673653No Hit
TGAGAAAATACCATGCACAAGATTATTAAATCTTTCATGTCCTCCTTCTT6080.10746302423573993No Hit
TTGAGTGAAGTTCTGCTTTAACAGGCACAGAATTCAAGCCTAGATCAGCA6070.10728627584061536No Hit
TGAGTCCAGCTTTGACCATCATCTGGTTGTAGAATTCGCCCAGTTTCACT6070.10728627584061536No Hit
TTGAGTACTGTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAA5880.10392805633324849No Hit
TTGAGTGCCAAGTCGTTTTCCCACCATCATGTACCATTTCTATCCCAATA5860.10357455954299935No Hit
TTGAGTAAAGTTGCAGAAATTATTCAGAAAGTAGCTGAAAAGGAGAGCTT5710.10092333361613076No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG100.007236465144.012029
AAGGTCG100.007236465144.012028
TTAACGC151.2636764E-4144.012026
GCACCGT450.0143.99935
GCCGCCT52100.0143.873811
CCGCCTT52500.0143.03932
CGCCTTA52500.0142.490743
CCTTACA52550.0141.670095
CTTACAC52850.0140.742136
TTACACT53650.0138.24087
TTAAGGT38350.0137.440411
ACACTAG10700.0137.28256
TAAGGTC38650.0136.734132
TACACTG54250.0136.711878
CACTAGG10750.0135.974157
AAGGTCT39750.0134.761613
ACACTGA55100.0134.210839
TCAAGAC11550.0133.413731
GATTCGA8050.0133.266425
CTAGGAT11150.0133.033529
Produced by FastQC (version 0.11.2)