FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_328_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_328_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2968522
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA40780.1373747609079535No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT40700.1371052665265745No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC38800.13070477496882285No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT30130.101498321386872No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT13450.086.720955
CGGTGCA4300.080.374572
AGCACGC14000.079.2197951
ACGCACT14750.078.589614
GTACGGC300.001989895272.001019
AACACGT8200.070.244896
AACACGA19500.068.308656
GCACGCA16850.067.088132
AAACACG28400.066.422355
GAAACAC30450.062.6599244
GCGGTGC12550.058.532551
TAACAAG43450.057.831835
ACACGTG10050.056.5978057
ACACGAG24200.055.0420957
GTAACAA48400.052.214734
AGCGGTG5850.045.5498431
ACGTGCC3650.045.37059
AAGCGGT3200.045.0013922
CACGCAC25400.044.7872773
CACGAGC30450.043.7444578
Produced by FastQC (version 0.11.2)