FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_310_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_310_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740014
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTGGCTCTGGGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCA670969.066855491923127No Hit
TCAAGCTCACTCTCCGCCGCCCCTAAAGACCTCTTCCTCCCTCCTCTGAA400645.413951627942174No Hit
TCAAGGATCTAGAACAAGAAATAACATTTGACCCAGTAATCCCATTACTG147591.9944217271565132No Hit
TCAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCT143991.9457739988702916No Hit
TTGAGTTAACGCTGGACGAGATGAGGACTCAGAGTCAAGATCAAGTTGCT116331.5719972865378222No Hit
TCAAGAACCCTGCAGAAATGGACAGCAGGCATCTCCAAAGTGACCTGTGT109651.4817287240511667No Hit
TGAGTGGCTCTGGGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCAA95851.2952457656206504No Hit
TTGAGTTAACGCTCCGGGGGGAAAATCATGGGGAAAATACTTAAAATGTC60500.8175521003656687No Hit
TCAAGGGCCTTTACTTTTGCAATTTTCCCTGCTTGGAGCAATCTTCCTCC57770.780660906415284No Hit
TTGAGTGTTTTGGAGGGAAAAGCAGTTTGTAAATGTTTGTTGAATGACAG56360.7616072128365139No Hit
TTCAGGAGGGTAATGAGAGTTGCAGCAAGTATAAATCTTGTCACAACCCC55850.7547154513292992No Hit
TTGAGTGAATCCTGCTGCTTGTTAGCCATCAGGGTTGGTGGTGGCACTGT45900.6202585356493256No Hit
TTGAGTTTATCTGAATTTTTCTCGTGTCTTCCTGCTCCTCCTTCCTTGTA45220.6110695203063726No Hit
TTGAGTCAACCCTGAACAGGCCACTGATGTGTGATGTTCCCCTCCCTGTG36850.4979635520409073No Hit
TCAAGGCTCTTTGTTGCGGCCGGGCGCCGTGGCTCACGCCTATAATCCCA35500.4797206539335742No Hit
TTGAGTTGTGTCCAGGTTTTGCGTGGCCATGGAAAATCAGGTGAAGGACA33730.45580218752618196No Hit
TTGAGTTAACGCTGAGGTGAGGACAGGAGTTGGAAAAGGGCCTGCCTGCT32790.4430997251403352No Hit
TTGAGTTAACGCTTTTCCTTATAAATTACACAATCTCAGTTATTTCTTTA28590.3863440421397433No Hit
TAGAGTGGCTCTGGGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCA27010.36499309472523495No Hit
TTGAGTCCAATAGCTGCCCCAGCTTACCACATTAATTAGTCTTCAGCCGA26600.35945265900374856No Hit
TTGAGTTAACGCTGTGGCATGGCTGAGGTGAGGACAGGAGTTGGAAAAGG26360.3562094771180005No Hit
TTGAGTTAACGCTGGACGCGATGAGGACTCAGAGTCAAGATCAAGTTGCT26240.35458788617512643No Hit
TTGAGTAAACAAATCTTAGTGAATTCAAGGACCTTCGAAAGAGGTTAGAG25060.33864224190353154No Hit
TCAAGTCTTCACAAGCGCTGATCATTTTTACCACCTGTAAGATACCTCTT22210.30012945701027277No Hit
TTGAGTTAACGCTGCAGAAATGGACAGCAGGCATCTCCAAAGTGACCTGT22130.29904839638169006No Hit
TTGAGTTAACGCTGCAGGAATGGACAGCAGGCATCTCCAAAGTGACCTGT21550.2912107068244655No Hit
TTGAGTCAAAGCTGCAATGTGGAGAGATGAAGGAAAAGAGAGAGCCAAAT21000.2837784150029594No Hit
TTGAGTTAACGCTGGGGGGAAAATCATGGGGAAAATACTTAAAATGTCTA20210.2731029412957052No Hit
TTGAGTCAAGGCAGATGAGCTGGAGAACACGGTGCTTCTGGGAAGCTGAA20130.2720218806671225No Hit
TTAAGATCCTATCTGAAGTTGAAGTGTATGGGTCACACGGCCGGCTTCAA19470.2631031304813152No Hit
GCCGCCTTACACTGACTTGAGTGGCTCTGGGAGGTCATGAGGGAGGGGAC18930.25580597123838195No Hit
CAAGCTCACTCTCCGCCGCCCCTAAAGACCTCTTCCTCCCTCCTCTGAAG18910.25553570608123627No Hit
TTGAGTTAACGCTGCAGACATGGACAGCAGGCATCTCCAAAGTGACCTGT16390.22148229628088117No Hit
TTGAGTTAACGCTAGCTGGACGAGATGAGGACTCAGAGTCAAGATCAAGT15990.21607699313796763No Hit
TTGAGTTTACGACTGTCGACCATTTCCCATGATCCAAACAGAGCCTAAGA15790.21337434156651092No Hit
TCAAGGGAAGTGGTGGGTAACGACAATATTGTCATAAATAGACTCACAGG15580.2105365574164813No Hit
TTGAGTAAACACAATCTGCGGGTAATTAGCATCATTCCACCTTTCAGGGA15560.21026629225933563No Hit
TCGAGATTGGGAATAGGTAATAAAGAAAATGAATCCTGAGGGCTGATAAA15510.20959062936647144No Hit
TTGAGTTAACGCTGCAGCAATGGACAGCAGGCATCTCCAAAGTGACCTGT15130.2044555913807036No Hit
TTGAGTTAACGCTGCCAGCTGGACGAGATGAGGACTCAGAGTCAAGATCA13720.18540189780193347No Hit
TTGAGTGAACTTCCCCCCCAGTACGGGCACCCAGAGTTGGTGCCGCGCTC13680.18486136748764212No Hit
TTGAGTCCTGTTTGGAGGACAGCTGCTCCCACATCTGGGGGCCTCTGGAT13100.17702367793041754No Hit
GCCGCCTTACACTGACTTGAGTGAATCCTGCTGCTTGTTAGCCATCAGGG12940.17486155667325212No Hit
TTGAGTTAACGCTGCAGATATGGACAGCAGGCATCTCCAAAGTGACCTGT12900.1743210263589608No Hit
TTGAGTTAACGCTGCTGAAATGGACAGCAGGCATCTCCAAAGTGACCTGT12740.17215890510179538No Hit
TTGAGTTAACGCTGCAGAGATGGACAGCAGGCATCTCCAAAGTGACCTGT11900.160807768501677No Hit
TTGAGTTAACGCTGCCGAAATGGACAGCAGGCATCTCCAAAGTGACCTGT11850.1601321056088128No Hit
CTGGGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCAATCCTGAGAG11060.1494566319015586No Hit
GTGAGTGGCTCTGGGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCA10810.14607831743723768No Hit
GCCGCCTTACACTGACTTGAGTTCAAGCTTCAGAGGAGGGAGGAAGAGGT10190.13770009756572174No Hit
CCCCAATCCTGAGAGAAAGGTCAGAGTAGCCTGGGGGAGCCAAGGGTTCC10080.13621363920142052No Hit
GGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCAATCCTGAGAGAAA9900.13378125278710942No Hit
TTGAGTTAACGCTTTAATTTGCATCTTTTACTGATATTTTTGGTATAGTT9720.13134886637279836No Hit
TTGAGTTAACGCTGTAGAAATGGACAGCAGGCATCTCCAAAGTGACCTGT9690.13094346863707984No Hit
GCTCTGGGAGGTCATGAGGGAGGGGACATGGGAATGAGCCCCAATCCTGA9630.1301326731656428No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTGTAGAATTCGCCCAGTTTCAC9550.1290516125370601No Hit
TTGAGTTAACGCTTGCAGAAATGGACAGCAGGCATCTCCAAAGTGACCTG9430.12743002159418607No Hit
TTGAGTTAACGCTGGAGAAATGGACAGCAGGCATCTCCAAAGTGACCTGT9300.12567329807273916No Hit
TTGAGTTAACGCTGGACGGGATGAGGACTCAGAGTCAAGATCAAGTTGCT9060.12243011618699107No Hit
CAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCTG8940.120808525244117No Hit
TCAAGGCCAGGGGAGCAGGAGAAAAGACAGAAAACTCATCTATCCAGGAT8920.12053826008697133No Hit
TCAAGCTCACTCTCCGCCGCCCCTAAAGCCCTCTTCCTCCCTCCTCTGAA8840.11945719945838863No Hit
TTAAGGCTCTTCTTATTCTAGGCTCAGCTGGACAACTCCAGATCCATCCT8770.11851127140837876No Hit
TTGAGTTAACGCTGCAGTAATGGACAGCAGGCATCTCCAAAGTGACCTGT8670.11715994562265038No Hit
TCAAGTGGTGGAAGCTCTAGGGCTTCCCAATCACAAGCTTATCCACTGTA8570.11580861983692202No Hit
CAAGGATCTAGAACAAGAAATAACATTTGACCCAGTAATCCCATTACTGG8450.11418702889404796No Hit
TTGAGTTGGAAAAGGGCCTGCCTGCTGAAAGGCTCCAGTCCTGTGTGTTG8290.11202490763688254No Hit
TTGAGTTAACGCTAATGCCCATCAATAATAGACTGGACAAAGAAAACGTG8220.11107897958687268No Hit
GCCGCCTTACACTGACTTGAGTGTTTTGGAGGGAAAAGCAGTTTGTAAAT8200.110808714429727No Hit
TGAGTTAACGCTGGACGAGATGAGGACTCAGAGTCAAGATCAAGTTGCTT7820.10567367644395917No Hit
GCCGCCTTACACTGACTTGAGTTAACGCTGGACGAGATGAGGACTCAGAG7800.10540341128681349No Hit
TCAAGACTTTCTAGAGGGCCCAAAACAAAAAAACCCCAATAACACATGCT7660.10351155518679377No Hit
TCAAGAGTGACTCAAAGTCAAAATCTGGCCTCACTTGCCAGTGAAAGGAC7560.10216022940106538No Hit
TTGAGTTAACGCTGAGTTGTGTCCAGGTTTTGCGTGGCCATGGAAAATCA7560.10216022940106538No Hit
TTAAGAAAGCACTTAGGCCTCTCCGAGACCGTTTTTCTCTTCTGTAAAAT7520.10161969908677403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC253.8669896E-8143.999946
CGCCTTA44400.0140.75673
CTTACAC44600.0140.286946
CCGCCTT44900.0139.34962
CCTTACA44850.0139.34445
GCCTTAC45050.0138.72584
TCGAGCA1300.0138.470831
TCAAGTG20150.0137.934811
GCCGCCT45300.0137.810581
TTACACT46100.0136.034657
TCAAGGG9550.0134.207961
ACACTGA46800.0133.23079
GGATCTA19450.0132.524375
TAGAGTG4800.0132.008851
CAAGCTC52400.0129.572452
GATCTAG19900.0129.527576
GTGAGTA19300.0129.450715
ATCTAGA20050.0128.558557
TTGAGTG110150.0127.340361
TTGAGTT240900.0127.181651
Produced by FastQC (version 0.11.2)