FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_310_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_310_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3381506
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC59690.17651898296202934No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA53490.158183957088942No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG48780.14425525194987085No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC45020.13313594593651468No Hit
GTAGAAACACGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC42050.12435287709085834No Hit
AAATTACACAATCTCAGTTATTTCTTTATAGCAACGCAAAAAATGGATGA38640.11426861286066031No Hit
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG38550.1140024592592768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA79250.0112.9585651
TAGTAAC82600.0108.6971362
GTAACAA100400.090.143264
AGTAACA107000.087.2746053
GCACGCA27950.085.523992
AGCACGC28200.084.788371
CGCACTT27500.084.5671165
TAACAAG109050.081.73855
AAGAGCG19450.073.6656659
ACGCACT37000.070.0539254
ACAAGAG127950.069.6083157
CAAGAGC130050.067.4323968
AAACACG74250.066.133215
AACAAGA139350.063.913776
AACACGT35300.062.209526
CACGCAC39550.060.6218833
GAAACAC81250.060.5241934
AACACGA51400.056.731416
ACACGTG41000.053.3852657
AAGAGCC65200.053.0060359
Produced by FastQC (version 0.11.2)