FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_302_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_302_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592781
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAGATTATCTCAGGCCCTGAACTTTTCAATTCACAAGTGAAATTCTAT265114.472309335150756No Hit
TTAAGATTCGAGAAGGCAGAATAATAAAAGAAATATTTCCAACAGGGAGA133082.2450112267430975No Hit
TCAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCT116961.9730726862028303No Hit
TTGAGTCCTGTTTGGAGGACAGCTGCTCCCACATCTGGGGGCCTCTGGAT92471.5599352880743478No Hit
TCAAGTAACCTTGTGGATGAGGGCTGGCAGGGCCTCTGCCTTTCAAACAT90681.5297386387215515No Hit
TCAAGCATGTATGCCAGTACAATGGGAGTTATCATCGTTCCTTTCAATTT76101.2837793384065952No Hit
TTGAGTCTCTGCTTGTGATTCTTTGGCTTTGTATCTAGGAGTGGAAGCGC66721.1255421479433383No Hit
TTGAGTCTTTGTGTCCAAAGCAAGAACTACTTCTGGATTTTTAGTCAAAT65601.106648155052203No Hit
TTAAGCTGCTGGATCTGTGGGGAAAACAAACATCTGAGGTCCAGAATTCG55220.9315413280790039No Hit
TTAAGGAATCTGCATTCACTAACCCCTGAAGCTGGGCCATCAGTTATCAT46660.7871372395538994No Hit
TTGAGTCCAATAGCTGCCCCAGCTTACCACATTAATTAGTCTTCAGCCGA41070.6928359714633229No Hit
TTAAGTGGTTTGAAGAACAGTAGGGAAGAGAACTATGGCTTGCCTTCCCT37780.6373348673456133No Hit
TCAAGACTTTCTAGAGGGCCCAAAACAAAAAAACCCCAATAACACATGCT35080.5917868487687695No Hit
TTAAGGCATTTTGCCTGTTGACTTCCTGGAAGGTCAAAGAATGGTAATAT34080.5749172122588275No Hit
TCAAGGCATTTTGCTTTTTGACTTTCTGGAGGGTGAAAGAATGATAACAT33180.5597345393998796No Hit
TTAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC32470.5477570974778206No Hit
TTGAGCCTTTGTGTCCAAAGCAAGAACTACTTCTGGATTTTTAGTCAAAT31480.531056157332978No Hit
TTAAGCATCCCAAAAAACTGTTGCCATGACCTTTGCTCTTGGCCAGGTCA30760.5189100190458197No Hit
TTGAGTGTTATTATCACTATATGTGTCAGCTTCATTGTCATACTTACTAT27310.46070977308651934No Hit
TCAAGCATGGTTCCTGGGGCAGCTACAGCAACATCACATGGGCTCTATCC26960.45480540030803956No Hit
TCAAGACACTAGGATGTTCCAAGGAGTGAGGGGTGAAATAAATTTTCTTA26710.4505879911805541No Hit
TTGAGAAAATACCATGCACAAGATTATTAAATCTTTCATGTCCTCCTTCT22800.3846277124266803No Hit
TTGAGTACTTGCAACAGATGGGATATGACTCACAGAATTGAAAATATTTA22580.3809163923944931No Hit
TTGAGTTTATCTGAATTTTTCTCGTGTCTTCCTGCTCCTCCTTCCTTGTA21950.37028852139322954No Hit
TCAAGGCTCTTTGTTGCGGCCGGGCGCCGTGGCTCACGCCTATAATCCCA20010.33756142656394184No Hit
TCAAGAAACTACTGATGGGTGTGTTCTGCCTTAACAGGGATATAAAGAAA18650.31461872091042054No Hit
TCAAGAATCATGTTCCTCTGATCAGTATTTAGTATAGATCTGTTCCTTTT17780.29994213714677087No Hit
TTGAGTCCCCAATGAGAAGGGAAAATGAGAACAAGGGAGAGATTTTTTTC17480.2948812461937883No Hit
TCAAGTCGTGTAGCGGAGAACACACACCTCTGAGAGATGATATCACAAAT15950.26907070233357683No Hit
TGAAGATGTTCCCCTTTAAAAGCCCACTCTGGTCTCTGCTTGAAAGACAA15770.26603416776178723No Hit
TTGAGTACTATGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAA14260.24056101663177462No Hit
TTGAGTGGCTGGCTGGAGACTGGAACATATACACTGGCATACAATTATAT14060.23718708932978622No Hit
TTAAGTTCCATCAAGTTCAAGACACTTTTATAAGTGGTACTACCAGCCAC13960.23550012567879203No Hit
TTAAGAAAGCACTTAGGCCTCTCCGAGACCGTTTTTCTCTTCTGTAAAAT13090.22082354191514234No Hit
TTGAGTGCTATATCGTCACTAATTGTCAGGGAAATGCAAATATAAAACAC13090.22082354191514234No Hit
TTGAGTCCAGTCCTGGTTCTATCTCTTAATACCTGTACCGTTTTGTACAA12750.215087865501762No Hit
TCGAGATTGGGAATAGGTAATAAAGAAAATGAATCCTGAGGGCTGATAAA12600.2125574200252707No Hit
TTGAGTGCTATATCCACAATATATAAAGAACTTCTACAAGTCAACAACAC12480.21053306364407767No Hit
TCAAGTACAGAATATAGATACAGCTTAAATTTTGTCTTACAAAGTCAGCA12340.20817131453268575No Hit
TTGAGTTCCCTTAAAAATAAATTAGAGAACAAAATTAAGTCAGAAAAAGC11400.19231385621334016No Hit
TTAAGATCCTATCTGAAGTTGAAGTGTATGGGTCACACGGCCGGCTTCAA11230.18944601800665No Hit
TTAAGTTAAAAAAATAAAGTTCACATTTTTTATGTTCATTATGTAATTCA10960.18489121614896564No Hit
TTGAGTGCTATATCCGGGTTATTAGTAGAAACACGAGCCAAAATAAAACT10940.1845538234187668No Hit
TTGAGTACTTTTCTAGACATTCTTTGGCTAATGTCTTTGGTTTTTTGGAC10310.17392595241750325No Hit
TTGAGGATTTCACTGGAACAGCTGTGTTTGACCTCCTCTTACAATTATTT10020.16903375782962005No Hit
GCCGCCTTACACTGACTTGAGTGTTATTATCACTATATGTGTCAGCTTCA10020.16903375782962005No Hit
TTAAGTTGAGAAATAAAGTTCACAGTTTCAAAAGGAAGGTATGCCATAAC9890.16684070508332757No Hit
TCGAGAATATTTAAATCATTGTCCCATCGTCTCCTAGCTTTCAAGATTGC9510.16043024320954957No Hit
TGAGTCCTGTTTGGAGGACAGCTGCTCCCACATCTGGGGGCCTCTGGATC9490.16009285047935073No Hit
TCAAGGTGATGTCAGGAATGCTATTAAGTGGCACCTTTTGAGGTGGCATG9270.15638153044716346No Hit
TGAGTCTCTGCTTGTGATTCTTTGGCTTTGTATCTAGGAGTGGAAGCGCT8960.1511519431290814No Hit
TTAAGCCATCTGCCCGCCTCGGCCTCCCACAGTGCTGGGATTACAGGAGT8840.14912758674788834No Hit
TAAGATTATCTCAGGCCCTGAACTTTTCAATTCACAAGTGAAATTCTATG8740.14744062309689415No Hit
CAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCTG8680.1464284449062976No Hit
TCGAGCATACAAAATAGGGAAAAGCACAAATCTGATAAGAGAAATACCCT8540.14406669579490572No Hit
TTAAGAGCACACAAGAAGCTATAAACAAGATAACAAAAAATCTCAACTCT8450.14254842850901092No Hit
TCAAGTAGGAGTTTCTGCCCTAGGACCGGTGGAGGGGATGGATTTGTCTC7980.13461969934933812No Hit
TTGAGCATCCCAAAATGTTGCCATGATCTTTGCTCTTGACTGGTCCGCAT7950.13411361025403987No Hit
TTGATGCCTCCACTCCCTTTGTCCCCATCAGATTCGCAAGGCCCTGTTAT7680.1295588083963555No Hit
TTAAGCCCTAACACATAGGTAATTAGATTAAACATAAATGGTTCTGAAAT7660.12922141566615664No Hit
TTGAGGAACAATGCTACGCTAAGTGTTGCAACCTTTTTGAGGCCTGTTTT7560.12753445201516245No Hit
TCAAGAGCATGGAATAGAGACTCCTAAGTATCTGGCTGATTTGTTTGATT7390.12466661380847227No Hit
TTCAGGGTGTGCGTCGGGGTCAGCCTCATGTTCCCAGAGCCCATCTGAGA7320.12348573925277631No Hit
TTGAGTGCTATATGGAGGGCAGTTGAATAATTTTAAAAAGGGGAATTACA7200.12146138287158327No Hit
TTAAGCTCTATCAACTTCAAGACACTACTGTAAGTAAAATGATATCAGCC7200.12146138287158327No Hit
TCAAGATGATGAATGACTCAATGGCCAAGAAAACCAGTGGAAATGCTTTC7130.12028050831588731No Hit
TTGAGTCACAAAGGCTAGAGAGGGGATGTGGGAGGAGAGAATGAGAGTCC6680.11268917188641336No Hit
TGAGTCTTTGTGTCCAAAGCAAGAACTACTTCTGGATTTTTAGTCAAATT6340.10695349547303304No Hit
TCAAGAAGGGAATTGCAGTGTTTTTAGCTATCTGCTTCTTGGGGGCACAG6060.10222999725024925No Hit
TTGAGAGGGAATTAGTTGCCAGGAGAAGGTTCCTGCCCGTGGCAGGAGCA5980.10088042632945388No Hit
TCGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG5960.10054303359925502No Hit
TTGAGTACTCAAATAAGGGAAAACAATATGTCTCTGACATCACAGGATGT5930.10003694450395678No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT100.0072383583144.05
GTACGTT100.0072383583144.05
GCCGCCT42450.0142.473511
CCGCCTT43000.0140.986042
CGCCTTA42950.0140.814913
GATTCGA16900.0138.035495
GCCTTAC43850.0137.760544
CGAGAAG16950.0137.628319
CCTTACA43850.0137.596345
ACACTGA43900.0137.275639
ATTCGAG17050.0136.82116
GCGATCC950.0136.421055
TTACACT45450.0133.386147
TAAGATT48700.0132.616012
TCAAGTG20050.0132.149631
TTAAGAT50850.0132.106191
TCAAGTA14500.0132.082761
TATCTCA32000.0132.075018
TGTAGCG1750.0131.657159
CTTACAC46300.0130.781856
Produced by FastQC (version 0.11.2)