FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_302_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_302_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2815698
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC64360.2285756498033525No Hit
AAGCAGAGCAGACTGCAGGAAGTCACGGCACCGGCCACAGAAGGGGGAAA60460.21472473255299396No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA57230.20325333185590216No Hit
GTAGAAACACGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC51450.18272556218742209No Hit
CAACAGCACTGGGTCCATGGGGCTCCGCTTCCTTCCCATGGCCTCCCCTC50280.17857028701231453No Hit
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG49150.17455707252695424No Hit
GCGGTGCAGACTGCAGGAAGTCACGGCACCGGCCACAGAAGGGGGAAAAG48690.17292337459486065No Hit
AGCACAGCCACTATGCTTGATTAGCATTATAGAGATGGTACAATGATGGT47530.16880361459218993No Hit
AGCACAGCCACTATGCTTGATTAGCATTATAGAGATGGTACGATGATGGT42290.15019366423529795No Hit
AAATTACACAATCTCAGTTATTTCTTTATAGCAACGCAAAAAATGGATGA42090.14948336078656163No Hit
CACGAGCACTGGGTCCATGGGGCTCCGCTTCCTTCCCATGGCCTCCCCTC38060.13517074629452447No Hit
AAATATAGTCATAACATTGTGCAGCCGTCCCTACCATCCATCTCCATAAT34250.1216394655960973No Hit
AAACAACAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAATCCTG30660.10888951869128011No Hit
GCAGAGCAGACTGCAGGAAGTCACGGCACCGGCCACAGAAGGGGGAAAAG29520.10484078903348298No Hit
GTAGAAACACGTGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC28590.10153787799685902No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA79950.0115.842981
TAGTAAC80400.0115.528282
GTAACAA93150.0101.642294
CGCACTT27900.099.096565
AGCACGC26300.096.1166461
TAACAAG98450.095.65855
AGTAACA103100.092.3218153
GCACGCA27850.092.299142
ACGCACT33050.086.9226464
CACGCAC32400.080.666493
CGGTGCA18250.079.302682
ACAAGAG121500.077.510957
AACACGT17650.077.506916
GCGGTGC27600.077.4987261
AAACACG55350.075.7071465
AACAAGA127800.073.746326
CAAGAGC125500.073.4341058
CCACTAT45950.072.548268
GAAACAC58100.072.123774
CACTATG48050.069.377589
Produced by FastQC (version 0.11.2)