FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_297_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_297_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930264
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGTCTCTTAATTCCT463974.98750892219843No Hit
TCAAGAATCATGTTCCTCTGATCAGTATTTAGTATAGATCTGTTCCTTTT221282.3786796006294986No Hit
TCAAGATGATGAATGACTCAATGGCTAAGAAAACCAGTGGAAATGCTTTC218472.3484731216084893No Hit
TTAAGATTCGAGAGGGCAGAATAATAAAAGAAATATTTCCAACAGGAAGA172561.8549573024431776No Hit
TTAAGATCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC165221.7760549693420362No Hit
TCAAGAACTGATAAGGCTCCCCATTGCTCCTTAGCTTTGGTGGTGAGAAA144881.5574073596312445No Hit
TTAAGGAATCTGCATTCACTAACCCCTGAAGCTGGGCCATCAGTTATCAT95721.0289552213135196No Hit
TTGAGTACTCATGTGTTCTGATCACGGTGTCTATTGTGTGGCCTGTTCCC95491.026482804881195No Hit
TTGAGGCTTGTATTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAA79640.856101063784044No Hit
TCGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG72640.7808536071480784No Hit
GCCGCCTTACACTGACTTGAACTAAAGAGAATATCAGGAAGAGGATTCAA65850.7078635742111917No Hit
TTGAGGAACAATGCTACGCTAAGTGTTGCAACCTTTTTGAGGCCTGTTTT62010.6665849694280334No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT61390.6599201946974192No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATAAGA58820.6322936284753575No Hit
TTAAGAGCACACAAGAAGCTATAAACAAGATAACAAAAAATCTCAACTCT56400.606279507752638No Hit
TTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGGA56280.6049895513531643No Hit
TTGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA50170.5393092713466284No Hit
TTGAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAA46660.5015780466620229No Hit
TCAAGAGCATGGAATAGAGACTCCTAAGTATCTGGCTGATTTGTTTGATT44360.47685388233877696No Hit
TCAAGGGGAGGTCAATGTGACTGGTGTGATACCACTAACAACAACACCAA44170.4748114513729436No Hit
CAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGTCTCTTAATTCCTT34320.3689275302494776No Hit
TCAAGTGCCAATAGATGCTCATCTTCACTGTTTATTATTCCTTCGTTGGA28560.30700962307474006No Hit
TTGAGAAAATACCATGCACAAGATTATTAAATCTTTCATGTCCTCCTTCT27470.295292519112854No Hit
TCAAGACACTAGGATGTTCCAAGGAGTGAGGGGTGAAATAAATTTTCTTA27430.2948625336463628No Hit
TCGAGCTGGATGAGAAAGTGGATGATCTCAGAGCTGACACTATAAGTTCA26300.28271544421798545No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA21320.22918225363982697No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAATAACGTTTCTTTGTAA18250.19618086908662485No Hit
TTGAGTCCTATGCTGGGTCCCTCTGCTGTAGACATAGGAAATGGATGCTT17450.18758115975680023No Hit
TTGAGTGTGTGTGCCTGGGTGTGTATATGAGTGTGAGTGTGCATAAGTGT17380.18682868519044055No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT16110.17317664662934393No Hit
TTGAGTCCTATGCCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTT15600.1676943319315807No Hit
TTGAGCTAAGGACCTTTGGACCATCCATGCTATGGTTCTTGGCATCCCCT14810.15920211896837888No Hit
CAAGATGATGAATGACTCAATGGCTAAGAAAACCAGTGGAAATGCTTTCA14760.15866463713526482No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA14490.155762235236449No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT13960.15006492780544017TruSeq Adapter, Index 27 (97% over 39bp)
TTGAGACCATCTGCATTTCTCGTCTCACTCCGGGCACTGAAGATCTCGCT13590.14608756224039626No Hit
GCCGCCTTACACTGACTTGGCACTCAGCAGCAACAGCCATTTATTGTTTA13520.14533508767403663No Hit
CAAGAATCATGTTCCTCTGATCAGTATTTAGTATAGATCTGTTCCTTTTA13470.14479760584092258No Hit
TTGAGTACTGTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAA13400.14404513127456292No Hit
TTGAGTCTCCATAGAGGTTCTTCATTTGGGTTTTGTTATCTGAATGGAAC13000.13974527660965058No Hit
TTGAGTACCATACATTTGTGCAGAAGGAGAAGACCAAATTACTGTTTGGG12270.13189804184618562No Hit
TCAAGAATAGTGCCTCCATGGCATTCTGTGAGGCTGCTTGAAACAGACCT12100.13007060361359785No Hit
TGAGGCTTGTATTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAAA12080.12985561088035225No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTTT11110.11942846331793985No Hit
GCCGCCTTACACTGACTTGATACCTAGTGCGGCTACTCCCAACACGGTAG11000.11824600328508897No Hit
TTGAGTACTCAAATAAGGGAAAACAATATGTCTCTGACATCACAGGATGT10660.11459112681991349No Hit
TGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGGAA9740.10470146109061514No Hit
TTGAGTCCTATGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTCCATGAA9620.10341150469114144No Hit
TCGAGATTGGGAATAGGTAATAAAGAAAATGAATCCTGAGGGCTGATAAA9360.10061659915894842No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG100.007239041143.999839
CGGGATA100.007239041143.999832
GCCGCCT66000.0141.186251
CCGCCTT67300.0138.97162
CGCCTTA67600.0138.141853
TCAAGGC58050.0137.572421
CCTTACA69200.0134.63575
CTTACAC69600.0133.551576
ATTCGAG20850.0132.604176
CGAGAGG20800.0131.884469
TCATGTT31450.0127.745488
GAATCAT31900.0127.523375
TTACACT73050.0127.145647
TTGAGTT29200.0127.0066451
GCCTTAC73600.0126.978124
GATTCGA22000.0125.0180365
AGGCCCA62150.0124.884814
TCAAGAA55450.0123.6338651
ACACTGA75200.0122.5530559
TCGAGTA8700.0121.674661
Produced by FastQC (version 0.11.2)