FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_297_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_297_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863562
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC161350.5634590764928435No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT97990.342196187824814No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA72850.254403431809753No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA64560.22545347368068158No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA61800.21581512815158185No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT57270.19999566972882027No Hit
CAACAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG52560.18354762355416088No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATG48480.16929963451114383No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA45070.15739138876685752No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT42610.14880068949092076No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACT41010.14321324280738465No Hit
GCACAGCATTTTCTTGTGAACTTCAAGTACCAGTAAAAGAACTGAAAATC40530.1415370088023238No Hit
ATATAAATCCTTACTTTCTCTTCATAGATGTACCCATACAGGCAGCAATT40110.1400703040478956No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC39520.13800993308334164No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATGT38850.13567018978461093No Hit
AGCACAGCATTTTCTTGTGAACTTCAAGTACCAGTAAAAGAACTGAAAAT37720.13172405556436353No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG37300.1302573508099353No Hit
CCCATACACTGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA37130.12966368459980962No Hit
ATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC36890.1288255675972792No Hit
ACAACAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAA35470.1238667086656409No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC34060.11894277127577473No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC33170.11583475405805777No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT33140.11572998943274145No Hit
CAACAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATTGTTCAAA31730.11080605204287527No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT31600.11035207199983797No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT31590.11031715045806588No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA31340.10944411191376334No Hit
GCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAATTGTTAAAACAAC31260.10916473957958653No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT30970.10815201486819562No Hit
GAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATTGTTAAAAC30780.10748850557452572No Hit
AGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATTGTTAAAACAA30600.1068599178226279No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT30450.1063360946960464No Hit
CACGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGATAAATTTA30350.10598687927832538No Hit
ATTGTATTCATTGAATTAATGGATAAATTTATATTAGCTCAAGGCCCACC30190.10542813460997177No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC30110.10514876227579496No Hit
CTTCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACA29640.10350744981250624No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC29330.10242488201757112No Hit
ATCCAATGATGACCAATAACCCCATATACATCTTCGGAGCTTATGTGTAC29040.1014121573061802No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC16800.0119.600351
CACGCAC18050.0114.08283
GCACGCA18000.0113.2016752
CGGTGCA2950.0109.832132
GAAACAC25500.0105.317364
CGCACTT20050.0104.4984745
ACGCACT20550.0102.6566544
GTAACAA66800.096.897954
AAACACG27950.096.085615
TAACAAG75900.085.849575
AACACGT6800.084.707146
CGCGGTG353.5541732E-582.305611
GTAGTAA80400.080.4373551
GAAGCGG7950.079.7173841
ACAAGAG82150.079.231857
TAGTAAC81300.079.086042
GCGGTGC16800.076.732841
ACAGCGT2350.076.595544
ACGTGCA13100.075.298832
CGGTGCG8150.073.326232
Produced by FastQC (version 0.11.2)