FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_284_sispa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_284_sispa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657503
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC8539012.987012987012985No Hit
TCAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCT423056.434191174793119No Hit
TTAAGAGCACACAAGAAGCTATAAACAAGATAACAAAAAATCTCAACTCT170832.5981630502066153No Hit
TTGAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAA149002.2661493559725203No Hit
TTAAGATTCGAGAAGGCAGAATAATAAAAGAAATATTTCCAACAGGAAGA141482.1517772542482696No Hit
TTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGGA126301.9209037829485187No Hit
TTGAGGTCTGGAAATTTAGTTTCTAACAAGTTTGTTTGTTGAAAAGGGCA111111.6898782210879644No Hit
TCAAGGAAAATGAAACATCTGTTCTACTGGGCTAACTCGGTTCTGAGTAA97761.486837322415259No Hit
TTGAGAAAATACCATGCACAAGATTATTAAATCTTTCATGTCCTCCTTCT79121.2033405170774887No Hit
TTAAGGAATCTGCATTCACTAACCCCTGAAGCTGGGCCATCAGTTATCAT69981.064329744503067No Hit
TCAAGGGGAGGTCAATGTGACTGGTGTGATACCACTAACAACAACACCAA68051.0349762662679867No Hit
TCGAGTATTTCGTTGTGGAGTTCATCCATGGCACCACTTAGCCTTTGAAG60160.9149768137940054No Hit
TTGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGA57330.871935185086608No Hit
TTGAGTCTCCATAGAGGTTCTTCATTTGGGTTTTGTTATCTGAATGAAAC56560.8602242119047365No Hit
TCAAGTGCCAATAGATGCTCATCTTCACTGTTTATTATTCCTTCGTTGGA54240.8249392017983187No Hit
TCAAGACACTAGGATGTTCCAAGGAGTGAGGGGTGAAATAAATTTTCTTA49500.752848275977448No Hit
TTGAGAACAAAACAATTTTATGAATTCAGAAATGGAAATAATAGTTTGCT40240.612012416673384No Hit
TCAAGTACAGAATATAGATACAGCTTAAATTTTGTCTTACAAAGTCAGCA35670.5425070303861732No Hit
TCAAGAGATACAGTGGTGGAAAAATATATCCCTATACTCATGAAGCTTAC31690.4819749871863703No Hit
TCGAGCTGGATGAGAAAGTGGATGATCTCAGAGCTGACACTATAAGTTCA30710.46707011222762485No Hit
TTGAGTAGAAGTAACGATCTCTTTGATCAATGGGGGTATGAGGAATCACC30270.4603781275522697No Hit
TTGAGTTAGACATCGTTCGGGAGTACCACCTTCCAATCTTGGATGCCATT29790.4530777806337006No Hit
CAAGTGAGTATAACTGCCATATTCAAAGATAGCCAGAGCTTTGGACTCTG28770.437564543431741No Hit
TCAAGAATCATGTTCCTCTGATCAGTATTTAGTATAGATCTGTTCCTTTT27900.4243326646418344No Hit
TAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCCG24160.36745079490131605No Hit
TTGAGTTGAAGAAATTGGTGGCAAATTCTGCCTCCTATAATATTACACTT20390.3101126534783872No Hit
TCAAGCATGTATGCCAGTACAATGGGAGTTATCATCGTTCCTTTCAATTT20090.30554993665428143No Hit
TCAAGTCTTCACAAGCGCTGATCATTTTTACCACCTGTAAGATACCTCTT17400.26463757579813324No Hit
TTGAGTTGGACATGATGCAAAAACCTGGGAAAACAGGAACAATTGTCTAT15490.23558827868466No Hit
TTGAGTTCATCCATGGCACCACTTAGCCTTTGAAGATTCTTTACTTCTAG14630.22250849045555685No Hit
TTGAGTTTTGTCTGCATTAACAGGCACAGAATTCAAGCCTAGATCAGCAT14250.21672904914502292No Hit
TTGAGTTTGACATCTCTGGCATTCTTTTTCTTCTGGAACTCGGTTTTCTT13090.19908654409181403No Hit
TTAAGTCCTGACCTCCCCAGTGGCTGGCAGATAAGGGTTTTTAAAGGCAG12870.1957405517541365No Hit
GCCGCCTTACACTGACTTGAGTAATAGAGCAGTATAGTATGGTTATCCAA12820.19498009895011886No Hit
TGAGTAAATTTCCTTTGACATCTGCATCACGTCCCTCAATATTCATTGAC12160.1849421219370862No Hit
TTGAGTCCAGCTTTGACCATCATCTGGTTGTAGAATTCGCCCAGTTTCAC12120.1843337596938721No Hit
TGAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAG11240.17094979034316193No Hit
TTGAGTGAGACGAGAAATGCAGATGGTCTCAGCTATGAACACAGCAAAAA11060.16821216024869848No Hit
TCAAGTCCATCCCTTCTTTTGTCTTCATAAGGGTCTCCCTTCCATCCTTT11010.16745170744468088No Hit
TTGAGTGGGCTTTCTGAAGATTACAGCAGCACTCTGACTTCCATGGCCCT10630.16167226613414692No Hit
TTGAGTAACCCATCCCCGGAAAGAGCAACTACAAGCAGTGAAGATGATGT10440.15878254547887993No Hit
TAAAGGTCTGCAGCAACTGCCACTCCATGTGCTCCGTGAGATGTGTATCC10400.15817418323566584No Hit
TTGAGTTGGACATTGCCCAATATGGGTGAAAACACCTTTGAAGCTTGCCA9960.15148219856031075No Hit
TTGAGTTGGACATCCGGGTGCTTCCCTATAATGCACGACAGAACAAAAAT9950.15133010799950722No Hit
TGAGAAAATACCATGCACAAGATTATTAAATCTTTCATGTCCTCCTTCTT9790.1488966590266508No Hit
GCCGCCTTACACTGACTTGAGTCTCCATAGAGGTTCTTCATTTGGGTTTT9750.14828829678343675No Hit
TTGAGTTGGACATGGTCACAGTGATTGAGTTGAAGAAATTGGTGGCAAAT9620.14631111949299092No Hit
TGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGGAA9570.14555066668897326No Hit
TTGAGTTGGACATGCTGGGTCCCTCTGCTGTAGACATAGGAAATGGATGC9440.14357348939852746No Hit
TCAAGAGCATGGAATAGAGACTCCCAAGTATCTGGCTGATTTGTTTGATT9350.14220467435129572No Hit
TGAGTCTCCATAGAGGTTCTTCATTTGGGTTTTGTTATCTGAATGAAACC9150.13916286313522525No Hit
TTAAGAAACGTGCATAATGTTACTAATGGAGCATCTCCATCTCCCAAGAG9100.13840241033120762No Hit
TTAAGATGGACAAACAGACTCTTTGCAGCAATAAGATACCAAAATTCCAA9100.13840241033120762No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT8600.13079788229103137TruSeq Adapter, Index 2 (97% over 37bp)
TGAAGGAGAGATTATCTTCTATGTATGTGCTAGCAGTAGCTCTGTTTCCA8540.1298853389262102No Hit
GCCGCCTTACACTGACTTGAGTTCATCCATGGCACCACTTAGCCTTTGAA8430.12821234275737145No Hit
TTGAGTTGGACATTGGTGGCAAATTCTGCCTCCTATAATATTACACTTTA8150.12395380705487276No Hit
GCCGCCTTACACTGACTTGAGTAAATTTCCTTTGACATCTGCATCACGTC8100.12319335425085512No Hit
TTGAGTTGGACATGCAAGAGCCATAGGAAATTGCCCAATATGGGTGAAAA7920.12045572415639169No Hit
TTGAGTTGGACATGCAAAAGCCATAGGAAATTGCCCAATATGGGTGAAAA7890.11999945247398111No Hit
TTGAGACTAGTAAGCAGCCCATAAGTCAGAAGCCAGTATATCAGAGTGGC7590.11543673564987536No Hit
CAAGGGGAGGTCAATGTGACTGGTGTGATACCACTAACAACAACACCAAC7560.11498046396746478No Hit
TCAAGATGATGAATGACTCAATGGCTAAGAAAACCAGTGGAAATGCTTTC7420.11285119611621544No Hit
TGAGGTCTGGAAATTTAGTTTCTAACAAGTTTGTTTGTTGAAAAGGGCAA7350.11178656219059076No Hit
TTGAGTTGGACATGCAGAAGCCATAGGAAATTGCCCAATATGGGTGAAAA7140.10859266041371675No Hit
TTGAGTGGCTGGCTGGAGACTGGAACATATACACTGGCATACAATTATAT7020.10676757368407444No Hit
TTGAGTTGGACATACGGTGGATTAAACAAAAGCAAGCCTTACTACACAGG6820.10372576246800394No Hit
TTGAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAACAATAAAA6800.1034215813463969No Hit
TTGAGTTACAATTCAAACAAAATTCTCACTACCCCAATTCTGATAACTTT6780.10311740022478984No Hit
TTGAGTTGGACATGGTCGCAGTGATTGAGTTGAAGAAATTGGTGGCAAAT6730.10235694742077224No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG100.007238537144.07
GTTAGTC100.007238537144.05
AGTTGCG100.007238537144.04
CGCCGTA100.007238537144.03
GCCGTAC100.007238537144.04
TACCGGC100.007238537144.09
CCGCCTT45950.0142.903152
GCCGCCT45900.0142.901961
CGCCTTA46150.0142.595893
TTAAGGT94550.0142.32471
TCAAGTG55600.0141.280581
TAAGGTC96350.0139.66582
CTTACAC47200.0138.966116
GGTCTGC99000.0138.545465
TCAAGGA11400.0138.31581
AAGGTCT99700.0137.717153
CCTTACA47700.0137.660375
ACACTGA48450.0135.232219
TCGAGAA17150.0135.183678
CGAGAAG17350.0134.040369
Produced by FastQC (version 0.11.2)