FastQCFastQC Report
Wed 18 Apr 2018
H2CYMAFXY_n01_284_nextera.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CYMAFXY_n01_284_nextera.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3700247
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA51700.13972040244880948No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC48420.1308561293340688No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG42370.11450586947303787No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA37950.10256072094646654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG35850.088.367965
AACACGT17750.084.778386
GAAACAC37750.084.111034
AACACGA21800.079.927476
AGCACGC12100.070.823121
ACACGAG25800.067.814697
ACACGTG20000.067.6807257
TAGAAAC80250.058.766992
GTAGTAA38500.057.2366451
GCACGCA16100.055.006812
ACAACGC10350.054.9571158
TAGTAAC39850.054.7458842
CGCACTT16700.053.460935
GTAACAA39900.052.8720364
ACACGTC2900.052.1384937
CACGAGC34000.050.8240788
GCGGTGC4900.049.96851
ACGAGCT5800.049.655719
ACGCACT20050.048.4786724
ACGTGCG901.3891495E-648.000522
Produced by FastQC (version 0.11.2)