FastQCFastQC Report
Mon 23 Dec 2019
H2CVWAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVWAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11420739
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG155460.13612078868101268No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT132360.1158944267967248TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC426900.072.892948
GAGCACA451700.068.430669
TCGGAAG484800.068.131643
CGGAAGA492450.067.292114
GATCGGA473750.066.87871
ATCGGAA501000.066.050922
AAGAGCA525300.061.2138527
GAAGAGC524850.061.197146
GGAAGAG543800.060.393695
CAGTCAC336750.039.94074626-27
GAACTCC355200.039.61102720-21
ACGTCTG373550.039.14021714-15
ACACGTC371450.039.03010612-13
GCACACG377900.038.82381410-11
CGTCTGA371750.038.38277416-17
CTGAACT380000.038.1543618-19
CACACGT382950.038.13986212-13
CACGTCT371250.038.13757314-15
CCAGTCA345050.037.4437426-27
AGCACAC399750.037.37661710-11
Produced by FastQC (version 0.11.5)